Protein Alignment sff and MELK

DIOPT Version :10

• Sequence 1: NP_648814.3
• Gene: sff / 39732
• FlyBaseID: FBgn0036544
• Length: 861
• Species: Drosophila melanogaster
• Sequence 2: NP_055606.1
• Gene: MELK / 9833
• HGNCID: 16870
• Length: 651
• Species: Homo sapiens
• Alignment Length: 785
• Identity: 190/785 - (24%)
• Similarity: 326/785 - (41%)
• Gaps: 203/785 - (25%)

  Fly    18 YRLEKTLGKGQTGLVKLGVHCVIGKKVAIKIINREKLSESVLMKVEREIAIMKLIDHPHVLGLSD 82
            |.|.:|:|.|....|||..|.:.|:.|||||:::..|. |.|.:::.||..:|.:.|.|:..|..
Human    11 YELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLG-SDLPRIKTEIEALKNLRHQHICQLYH 74

  Fly    83 VYENKKYLYLILEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLL 147
            |.|....::::||:..|||||||::.:.||:.:|.|..||||:||:.:.||....||||||||||
Human    75 VLETANKIFMVLEYCPGGELFDYIISQDRLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLL 139

  Fly   148 LDEKNNIKIADFGMASLQPAGSM---LETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILYALL 209
            .||.:.:|:.|||:.: :|.|:.   |:|.|||..||.||:|:|:.|.|.:|||||.|::||.|:
Human   140 FDEYHKLKLIDFGLCA-KPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLM 203

  Fly   210 VGALPFDDDNLRQLLEKVKRGVFHIPHFVPPDCQSLLRGMIEVNPDRRLTLAEINRHPWVTAGGK 274
            .|.|||||||:..|.:|:.||.:.:|.::.|....||:.|::|:|.:|:::..:..|||:.....
Human   204 CGFLPFDDDNVMALYKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKKRISMKNLLNHPWIMQDYN 268

  Fly   275 GELELEL--PMMEVVQTHVIPTATAVDPDVLNAICSLGCFKEKEKLIQELLSSSHNTEKVIYFLL 337
            ..:|.:.  |.:.            :|.|.:..:..  ..:...:.:::|:|..........:||
Human   269 YPVEWQSKNPFIH------------LDDDCVTELSV--HHRNNRQTMEDLISLWQYDHLTATYLL 319

  Fly   338 LERKRRRPALEDDDEIAQKSRSELDAVDPPRKRLDTCRINGTNAPSYGQIS------EGSPLTPR 396
            |              :|:|:|.:     |.|.||.:......:|..:..|.      |....:.:
Human   320 L--------------LAKKARGK-----PVRLRLSSFSCGQASATPFTDIKSNNWSLEDVTASDK 365

  Fly   397 RQAFNFRSY-----------SSTRNHQRRSPTTVTSSVRSSSYHSPTRCNSPMSSAQQQANAISR 450
            ........|           ::.|..|.....|.::.|.|.|. :|..|.:|.:..:.:.| :..
Human   366 NYVAGLIDYDWCEDDLSTGAATPRTSQFTKYWTESNGVESKSL-TPALCRTPANKLKNKEN-VYT 428

  Fly   451 PSSPAAGTRHSTYGDRDRSGHHSSVSRTPSHSSQKSIEGDVVVVREPRIERRDSLRQERGGGSP- 514
            |.|......:..:.:          .:||.:.:|               .:|:.|.......:|
Human   429 PKSAVKNEEYFMFPE----------PKTPVNKNQ---------------HKREILTTPNRYTTPS 468

  Fly   515 RDRGDCGIPPGSPGGNSSGSTSASPSVHHRANSGPTIAISMFHDPDSNSVVNPNGSPMMNNSSPG 579
            :.|..|              ...:|           |.|
Human   469 KARNQC--------------LKETP-----------IKI-------------------------- 482

  Fly   580 MPGSPCNTPGGQLWKTRLTNIKNSFLGSPRFHRRKMQVSADEVHLTPESSPELTKRSWFGNLITT 644
                |.|:.|.....|.:.        ||....|.:::..::.|:  |.:|:......||:|
Human   483 ----PVNSTGTDKLMTGVI--------SPERRCRSVELDLNQAHM--EETPKRKGAKVFGSL--- 530

  Fly   645 EK--DETFTILVKGKPIAT-------VKAH-------------LIHAFLSMAELSHSVVSPTSFR 687
            |:  |:..|:|.:.|...:       :|.|             |::..:|:....|
Human   531 ERGLDKVITVLTRSKRKGSARDGPRRLKLHYNVTTTRLVNPDQLLNEIMSILPKKH--------- 586

  Fly   688 VEYKRNGN----------GPVMFQRHVKFQVDISAICKQGDIADMLFALTFTLLSGNIRRFRRIC 742
            |::.:.|.          |.|..|    |::::..:.|. |:.    .:....|.|:...::|:.
Human   587 VDFVQKGYTLKCQTQSDFGKVTMQ----FELEVCQLQKP-DVV----GIRRQRLKGDAWVYKRLV 642

  Fly   743 EHIQS 747
            |.|.|
Human   643 EDILS 647

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
sff	NP_648814.3	STKc_BRSK1_2	16..269	CDD:270983	109/253 (43%)
		UBA_BRSK	298..350	CDD:270525	7/51 (14%)
MELK	NP_055606.1	STKc_MELK	7..263	CDD:270980	109/253 (43%)
		UBA_MELK	281..332	CDD:270526	11/83 (13%)
		UBA-like	282..321		7/54 (13%)
		Autoinhibitory region	326..651		69/440 (16%)
		MELK_C	555..649	CDD:213383	19/111 (17%)