Protein Alignment pHCl-1 and nAChRalpha3

DIOPT Version :10

• Sequence 1: NP_001261898.1
• Gene: pHCl-1 / 39730
• FlyBaseID: FBgn0264908
• Length: 978
• Species: Drosophila melanogaster
• Sequence 2: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Alignment Length: 805
• Identity: 143/805 - (17%)
• Similarity: 266/805 - (33%)
• Gaps: 253/805 - (31%)

  Fly   338 KEILDLLLEKKRYDKRLLPPVNDEDFCCGLQSPDMATNQNARRPHNRGTLTVNVNVLLLSLASPD 402
            |.:.|.||  ..|:|.:.|.||..|                       .|||.:.:.|..|.
  Fly    27 KRLYDDLL--SNYNKLVRPVVNVTD-----------------------ALTVRIKLKLSQLI--- 63

  Fly   403 ESSLKYEV---EFLLNQQWNDPRLQYGNKSHYDFLNALH-HHDSIWTPDTYFIMHGDFKDPIIPM 463
            :.:||.::   ...:.|.|.|.:|::..| .|..:..|| ..|.||.||.....:.|       .
  Fly    64 DVNLKNQIMTTNLWVEQSWYDYKLKWEPK-EYGGVEMLHVPSDHIWRPDIVLYNNAD-------G 120

  Fly   464 HFALRIFRNGTITYAMR---RHLIL---SCQGSLHIFPFDDPKCSFSMESISYE--EAQIKYVWK 520
            :|.:.:....|:.|..|   |...:   ||:..:..||||:..|.....|.:|:  :..::::.:
  Fly   121 NFEVTLATKATLNYTGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDE 185

  Fly   521 -NDEDTLRKSPSLT---------TLNAYLIKNQT--TACDQNSWRGNYSCLQVELTFTRDRAYYF 573
             |..:.:.....|:         .|....::|:.  |.||:     .|..:...:|..|...:|.
  Fly   186 LNGTNVVEVGVDLSEFYTSVEWDILEVPAVRNEKFYTCCDE-----PYLDITFNITMRRKTLFYT 245

  Fly   574 TTVFIP--GIILVTSSFITFWL-----EWNAVPARVMIGVTT-----------------MLNFFT 614
            ..:.||  ||..:|  .:.|:|     |..::...:::.:|.                 :|..|.
  Fly   246 VNLIIPCMGISFLT--ILVFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFV 308

  Fly   615 TSNGFRSTLPVVSNLTAMNVWDGVCMCFIYASLLEFVCVNYVGR----KRPLHNVVYRPGENPVT 675
            .......|..:...:..:|:..........|..:..|.:|.:.|    :|||:         |::
  Fly   309 LFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLY---------PIS 364

  Fly   676 QRL-------------------------PAVLSRIGVILASPLASAAATP--------------- 700
            :.:                         |..|||:.   .||..::.:|.
  Fly   365 EMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMH---HSPKTTSRSTSPHLQHRVQTYNLMDE 426

  Fly   701 -AMSMMGGATP----MSSGTPAASSSV--ATPGTPRITDPEEFFGGGMRWQEAHGGIIGAAVQNL 758
             .|.|.|||:.    :||..|...|:.  .|..|..:.|.:..    .::|:..|.::...:...
  Fly   427 CGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYH----NQYQQTGGSLLDHPLTPT 487

  Fly   759 RARSIKRKAAR-------------------------------SPSTSISPHPSGRPTEHIYEEPG 792
            :.|.:...:::                               |.|.:.||.|     :|.::...
  Fly   488 KFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSANFSPLP-----QHAHQAHA 547

  Fly   793 GSTSGVTSTKVKFKDELKEEQPEEAESSTKLRRSVATSTPAL------VVRIEAEIDPEA----- 846
            .:|:          .:|....|...:|:|.: .||||::...      .::|...:...|
  Fly   548 STTT----------SDLGMANPNVIKSTTTV-NSVATASTLADSCCSGTIQIHQGMGAGAACQSS 601

  Fly   847 ----------EDKPIQDMEMTERQLSLPLKPPRRKASRS--------NSPASVYSDAFENVEATP 893
                      :....|..:..::|.....:|....|:.:        ::.|:..:.|......||
  Fly   602 ELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTP 666

  Fly   894 GEKRQNAGAPNEIVACTTCGGSNSPCTHSANNGCATETC-----------FV--QVRKKEPPHPI 945
            ......||..:.:.||...||.:. |:...|......||           ::  |.||:|....:
  Fly   667 PPPPPPAGVCDILPACQREGGPHC-CSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEESTRV 730

  Fly   946 R-----VAKTIDVIARITFPTAYAI 965
            :     ||..:|.:....|..|..:
  Fly   731 KEDWKYVAMVLDRLFLWIFTIAVVV 755

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
pHCl-1	NP_001261898.1	LIC	339..>662	CDD:455710	73/374 (20%)
		LGIC_TM	<948..975	CDD:476813	4/18 (22%)
		TM4 helix	950..971	CDD:349850	3/16 (19%)
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	55/256 (21%)
		Cys-loop	150..164	CDD:349832	5/13 (38%)
		LGIC_TM_nAChR	242..>331	CDD:349866	14/90 (16%)
		TM1 helix	242..266	CDD:349866	7/25 (28%)
		TM2 helix	274..295	CDD:349866	1/20 (5%)
		TM3 helix	306..328	CDD:349866	2/21 (10%)
		Neur_chan_memb	<683..760	CDD:460753	15/74 (20%)