Protein Alignment AGO2 and Piwil1

DIOPT Version :10

• Sequence 1: NP_730054.1
• Gene: AGO2 / 39683
• FlyBaseID: FBgn0087035
• Length: 1217
• Species: Drosophila melanogaster
• Sequence 2: NP_067286.1
• Gene: Piwil1 / 57749
• MGIID: 1928897
• Length: 862
• Species: Mus musculus
• Alignment Length: 991
• Identity: 231/991 - (23%)
• Similarity: 380/991 - (38%)
• Gaps: 213/991 - (21%)

  Fly   300 RPSGQQQGGHQQGRQGQEGGY-QQRPSGQQQGGHQQGRQGQEGGYQQRPSGQQQ----GGHQQGR 359
            |..||:...|......|:.|| ..||......|...||..|.|......|..|:    .|.|:..
Mouse    12 RARGQETVQHVGAAASQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISAGFQELS 76

  Fly   360 QGQEGGYQQRPPGQQPNQTQSQGQYQSRGPPQQQQAAPLPLPPQPAGSIKRGTIGKPG---QVGI 421
            ..:.||.::              .:...|...:|.          ...:|....|..|   ::..
Mouse    77 LAERGGRRR--------------DFHDLGVNTRQN----------LDHVKESKTGSSGIIVKLST 117

  Fly   422 NYLDLDLSKMPSVAYHYDVKIMPERPKKFYRQAFEQFRVDQLGGAVLAYDGKASCYSVDKLPLNS 486
            |:..| .|:.....|.|.:...|....:..|.|. .|:.:.|.|...|:||     ::..||...
Mouse   118 NHFRL-TSRPQWALYQYHIDYNPLMEARRLRSAL-LFQHEDLIGRCHAFDG-----TILFLPKRL 175

  Fly   487 QN--PEVTVTDRNGRTLRYTI----EIKETGDSTIDLKSLTTYMNDRIFDKPMRAMQCVEVVLAS 545
            |:  .||....|||..:|.||    |:..|..:.:...::       ||.:.::.|...:
Mouse   176 QHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNI-------IFRRLLKIMNLQQ----- 228

  Fly   546 PCHNKAIRVGRSFFKMSD----PNNRHELDDGYEALVGLYQAFMLGDRPFLNVDISHKSFPISMP 606
                    :||:::..||    ||:|..:..|:...:..|:     :...|..|:|||
Mouse   229 --------IGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYE-----NNIMLCTDVSHK------- 273

  Fly   607 MIEYLERFSLKAKINNTTNLDY---------SRRFLEPF---LRGINVVYTPPQSFQSAPRVYRV 659
                        .:.:.|.||:         ..:|.|..   |.|: :|.|...:     :.|||
Mouse   274 ------------VLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGL-IVLTKYNN-----KTYRV 320

  Fly   660 NGLSRAPASSETFEH-DGKKVTIASYFHSRNY-----PLKFPQL-----HCLNVGSSI--KSILL 711
            :.:........||:. ||.:|:...|:. :.|     .||.|.|     .....|.::  .::|:
Mouse   321 DDIDWDQNPKSTFKKADGSEVSFLEYYR-KQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLI 384

  Fly   712 PIELCSIEEGQALNRKDGATQVANMIKYAATSTNV----RKRKIMNLLQYF--QHNLDPTISRFG 770
            | |||.:.......|.|     .|::|..|..|.:    |:|::..|:.|.  ..|:...:..:|
Mouse   385 P-ELCYLTGLTDKMRND-----FNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWG 443

  Fly   771 IRIANDFIVVSTRVLSPPQVEYHSKRFTM-VKNGSWRMD--GMKFLEPKPKAHKCAVLYCDPRSG 832
            :...::.:..|.|:|...::....|.|.. .:...|..:  |...:..|| .....::|
Mouse   444 LSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKP-LDNWLLIY------ 501

  Fly   833 RKMNYTQLND-FGNLI-------ISQGKAVNISLDSDVTYRPFTDDERSLDTIFADLKRSQHDLA 889
            .:.||...|. ..||.       |...||:.|.:           |:|:...:.|..::...|..
Mouse   502 TRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEV-----------DDRTEAYLRALQQKVTSDTQ 555

  Fly   890 IVII---PQFRISYDTIKQKAELQHGILTQCIKQFTVERKCNNQTI----GNILLKINSKLNGIN 947
            ||:.   ...:..||.||:.........:||:...|:.::   ||:    ..|.|::|.|:.|..
Mouse   556 IVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQ---QTVMAIATKIALQMNCKMGGEL 617

  Fly   948 HKIKDDPRLPMMKNTMYIGADVTHPSPDQREIPSVVGVAASHDPYGASYNMQYRLQ-RGALEEIE 1011
            .:: |.|    :|..|.:|.|..|.:...|.  |:.|..||.:.....:..:...| ||  :|:.
Mouse   618 WRV-DMP----LKLAMIVGIDCYHDTTAGRR--SIAGFVASINEGMTRWFSRCVFQDRG--QELV 673

  Fly  1012 DMFSITLE-HLRVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEE----LRCIKQACDKVGCKPKI 1071
            |...:.|: .||.:.......|..:|.|||||.|||...:.|.|    |.|:|..  ..|..|::
Mouse   674 DGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSV--GRGYNPRL 736

  Fly  1072 CCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGTAKPTRYNV 1136
            ..::|.||.:.|||    ..:..:..|..||||:|..:..|....||:||.....|:..||.|||
Mouse   737 TVIVVKKRVNARFF----AQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNV 797

  Fly  1137 IENTGNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRVYLTGTNRFLDLKKEYAK 1201
            |.::..|..|.:|:|||.|||::......:..|||...||.:|     :|.|.:    :.:|
Mouse   798 IYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLA-----FLVGQS----IHRE--- 850

  Fly  1202 RTIVPEFMKKNPMYFV 1217
                |.....|.:|::
Mouse   851 ----PNLSLSNRLYYL 862

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO2	NP_730054.1	ArgoN	471..543	CDD:465134	18/77 (23%)
		ArgoL1	554..601	CDD:462567	14/50 (28%)
		PAZ	605..719	CDD:239207	29/138 (21%)
		Piwi_ago-like	764..1186	CDD:240015	116/445 (26%)
Piwil1	NP_067286.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..48		10/35 (29%)
		GAGE	16..107	CDD:461753	21/114 (18%)
		D-box. /evidence=ECO:0000269|PubMed:23328397	218..225		0/6 (0%)
		ArgoL1	229..276	CDD:462567	14/70 (20%)
		PAZ	278..416	CDD:198017	35/150 (23%)
		Required for binding 2'-O-methylated 3'-end of piRNAs. /evidence=ECO:0000269|PubMed:21237665	317..319		0/1 (0%)
		Piwi_piwi-like_Euk	402..845	CDD:240016	126/483 (26%)
		MID region. /evidence=ECO:0000305|PubMed:24757166	480..616		32/156 (21%)