Protein Alignment AGO2 and PIWIL3

DIOPT Version :10

• Sequence 1: NP_730054.1
• Gene: AGO2 / 39683
• FlyBaseID: FBgn0087035
• Length: 1217
• Species: Drosophila melanogaster
• Sequence 2: NP_001008496.2
• Gene: PIWIL3 / 440822
• HGNCID: 18443
• Length: 882
• Species: Homo sapiens
• Alignment Length: 954
• Identity: 223/954 - (23%)
• Similarity: 378/954 - (39%)
• Gaps: 193/954 - (20%)

  Fly   357 QGRQGQEGGYQQRPPG--QQPNQ--TQSQGQYQSRGPPQQQQAAPLPLPPQPAGSIKRGTIG--- 414
            :||..:...|||..||  :.|..  ||...|.||...|.|:: .|:..|.||..:  ||..|
Human    10 RGRARRRESYQQEAPGGPRAPGSATTQEPPQLQSTPRPLQEE-VPVVRPLQPRAA--RGGAGGGA 71

  Fly   415 ------KPG-------------QVGINYLDL-------------------------------DLS 429
                  :||             ::|..:.||                               .:|
Human    72 QSQGVKEPGPEAGLHTAPLQERRIGGVFQDLVVNTRQDMKHVKDSKTGSEGTVVQLLANHFRVIS 136

  Fly   430 KMPSVAYHYDVKIMPERPKKFYRQ-AFEQFRVDQLGGAVLAYDGKASCYSVDKLPLNSQNPEVTV 493
            :...|||.|:|...|:......|. ..:|.|  :..|....:||.:...|   .||..:..|...
Human   137 RPQWVAYKYNVDYKPDIEDGNLRTILLDQHR--RKFGERHIFDGNSLLLS---RPLKERRVEWLS 196

  Fly   494 TDRNGRTLRYTIEI-KETGDSTIDLKSLTTYMNDRIFDKPMRAMQCVEVVLASPCHNKAIRVGRS 557
            |.::...::.|:|. ||...::.|   ...|.| .:|.:..:.:...:             |||:
Human   197 TTKDKNIVKITVEFSKELTPTSPD---CLRYYN-ILFRRTFKLLDFEQ-------------VGRN 244

  Fly   558 FF--KMSDPNNRH----ELDDGYEALVGLYQAFMLGDRPFLNVDISHKSFPISMPMIEYLERFSL 616
            ::  |.:....||    |:..||...|..|:..:.     |..|:|||...|. ...::::|.|.
Human   245 YYTKKKAIQLYRHGTSLEIWLGYVTSVLQYENSIT-----LCADVSHKLLRIE-TAYDFIKRTSA 303

  Fly   617 KAKINNTTNLDYSRRFLEPFLRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETF-EHDGKKVT 680
            :|:..|.      |..:...|.| ::|.|...:     :.|||:.:.......:|| :.||.|:|
Human   304 QAQTGNI------REEVTNKLIG-SIVLTKYNN-----KTYRVDDIDWKQNPEDTFNKSDGSKIT 356

  Fly   681 IASYFHSRNYPL----KFPQLHCLN------VGSSIKSILLPIELCSIEEGQALNRKDGATQVAN 735
            ...|:..::..:    |.|.|....      .|:..:.|||..:||.:     ....|...:..:
Human   357 YIDYYRQQHKEIVTVKKQPLLVSQGRWKKGLTGTQREPILLIPQLCHM-----TGLTDEICKDYS 416

  Fly   736 MIKYAATSTNVRKRKIMNLLQYFQHNLDPT------ISRFGIRIANDFIVVSTRVLSPPQVEYHS 794
            ::|..|..|.:..|:..:.|:.|.:.|...      :..:.::...:|:.|..|||....:....
Human   417 IVKELAKHTRLSPRRRHHTLKEFINTLQDNKKVRELLQLWDLKFDTNFLSVPGRVLKNANIVQGR 481

  Fly   795 KRFTMVKNGSWRMD--GMKFLEPKPKAHKCAVLYCDPRSGRKMNYTQLNDFGNLIISQGKAVNIS 857
            :.......|.|..:  .:..|...| .|...:||  .||..:          ..:..:|...:::
Human   482 RMVKANSQGDWSREIRELPLLNAMP-LHSWLILY--SRSSHR----------EAMSLKGHLQSVT 533

  Fly   858 LDSDVTYRPFT------DDERSLDTIFADLKRSQHDL-----------AIVIIP-QFRISYDTIK 904
            ....:|.:|..      |....:||:   .|.::..|           .|.|:| ..:..||:||
Human   534 APMGITMKPAEMIEVDGDANSYIDTL---RKYTRPTLQMGMSCLLVFKVICILPNDDKRRYDSIK 595

  Fly   905 QKAELQHGILTQCIKQFTVERKCNNQTIGNILLKINSKLNGINHKIKDDPRLPMMKNTMYIGADV 969
            :....:..|.:||:.:.|:|:......:..|..::|.|:.|...|::.|     ::.||::|.|.
Human   596 RYLCTKCPIPSQCVVKKTLEKVQARTIVTKIAQQMNCKMGGALWKVETD-----VQRTMFVGIDC 655

  Fly   970 THPSPDQREIPSVVGVAASHDPYGASYNMQYRLQRGALEEIEDMFSITLEHLRVYKEYRNAYPDH 1034
            .|...::::  |:.|..||.:.....:..|..:|:...|.::::.......|.|:.:..::.|..
Human   656 FHDIVNRQK--SIAGFVASTNAELTKWYSQCVIQKTGEELVKELEICLKAALDVWCKNESSMPHS 718

  Fly  1035 IIYYRDGVSDGQFPKIKNEELRCIKQACDKVGCKPK---ICCVIVVKRHHTRFFPSGDVTTSNKF 1096
            :|.|||||.|||...:.:.|.:  |.:.......|.   :..::|.||.:||||    :...:.|
Human   719 VIVYRDGVGDGQLQALLDHEAK--KMSTYLKTISPNNFTLAFIVVKKRINTRFF----LKHGSNF 777

  Fly  1097 NNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGTAKPTRYNVIENTGNLDIDLLQQLTYNLCHMFPR 1161
            .|..||||:|..:.......||:||.....||..||.||||.:|..|..|.:|:|||.||||:..
Human   778 QNPPPGTVIDVELTRNEWYDFFIVSQSVQDGTVTPTHYNVIYDTIGLSPDTVQRLTYCLCHMYYN 842

  Fly  1162 CNRSVSYPAPAYLAHLVAARGRVYLTG------TNRFLDLKKEY 1199
            ....:..|||.:.||.:|     ||.|      .||.|..:..|
Human   843 LPGIIRVPAPCHYAHKLA-----YLVGQSIHQEPNRSLSTRLFY 881

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO2	NP_730054.1	ArgoN	471..543	CDD:465134	15/72 (21%)
		ArgoL1	554..601	CDD:462567	16/52 (31%)
		PAZ	605..719	CDD:239207	28/124 (23%)
		Piwi_ago-like	764..1186	CDD:240015	110/450 (24%)
PIWIL3	NP_001008496.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..91		24/83 (29%)
		PAZ_piwi_like	278..408	CDD:239211	34/152 (22%)
		Piwi_piwi-like_Euk	416..865	CDD:240016	119/482 (25%)