DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment AGO2 and AGO1

DIOPT Version :9

Sequence 1:	NP_730054.1	Gene:	AGO2 / 39683	FlyBaseID:	FBgn0087035	Length:	1217	Species:	Drosophila melanogaster
Sequence 2:	NP_001304051.1	Gene:	AGO1 / 26523	HGNCID:	3262	Length:	891	Species:	Homo sapiens

Alignment Length:	838	Identity:	278/838 - (33%)
Similarity:	416/838 - (49%)	Gaps:	77/838 - (9%)

- Green bases have known domain annotations that are detailed below.

  Fly   389 PPQQQ--QAAPLPLPPQPAGSIKRGTIGKPGQVGINYLDLDLSKMPSVAYHYDVKIMPER-PKKF 450
            ||.||  ||     |.:|.    .||:|||.::..||.::|:.|:.  .|||:|.|.|:: |::.
Human    15 PPLQQVFQA-----PRRPG----IGTVGKPIKLLANYFEVDIPKID--VYHYEVDIKPDKCPRRV 68

  Fly   451 YRQAFE----QFRVDQLGGAVLAYDGKASCYSVDKLPLNSQNPEVTVT-DRNGRTLRYTIEIKET 510
            .|:..|    .|:....|.....||||.:.|:|..||:.::..:..|| ...|:...:.:.||..
Human    69 NREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWL 133

  Fly   511 GDSTIDLKSLTTYMNDRIFDKPMRAMQCVEVVLASPCHNKAIRVGRSFFKMSDPNN-RHELDDGY 574
              :.:..:.|...:.......|:.::|.::|.:......:...||||||  |.|.. .|.|..|.
Human   134 --AIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFF--SPPEGYYHPLGGGR 194

  Fly   575 EALVGLYQAFMLGDRP-----FLNVDISHKSFPISMPMIEYLERFSLKAKINNTTNLDYSR---- 630
            |...|.:|:.    ||     .||:|:|..:|..:.|:||::      .::.:..|:|...
Human   195 EVWFGFHQSV----RPAMWKMMLNIDVSATAFYKAQPVIEFM------CEVLDIRNIDEQPKPLT 249

  Fly   631 -----RFLEPFLRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETFE---HDGKKV--TIASYF 685
                 ||.:. ::|:.|..|.....:   |.|||..::|.|||.:||.   ..|:.|  |:|.||
Human   250 DSQRVRFTKE-IKGLKVEVTHCGQMK---RKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYF 310

  Fly   686 HSR-NYPLKFPQLHCLNVGSSIKSILLPIELCSIEEGQALNRKDGATQVANMIKYAATSTNVRKR 749
            ..: |..||:|.|.||.||...|...||:|:|:|..||...:|....|.:.|||..|.|...|:.
Human   311 KQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQE 375

  Fly   750 KIMNLLQYFQHNLDPTISRFGIRIANDFIVVSTRVLSPPQVEY--HSKRFTMVKNGSWRMDGMKF 812
            :|..|::...:||||.|..|||::.:|...|:.|||..|.::|  .::.......|.|.|.|.:|
Human   376 EISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQF 440

  Fly   813 ---LEPKPKAHKCAVLYCDPRSGRKMNYTQLNDFGNLIISQGKAVNISLDSDVTYRPFTDDERSL 874
               :|.|..|..|..    |:  ::.....|.:|.:.:....|...:.:.....:..:.....|:
Human   441 YNGIEIKVWAIACFA----PQ--KQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSV 499

  Fly   875 DTIFADLKR--SQHDLAIVIIPQFRISYDTIKQKAELQHGILTQCIKQFTVERKCNNQTIGNILL 937
            :.:|..||.  |...|.|||:|.....|..:|:..:...|:.|||::...|. |.:.||:.|:.|
Human   500 EPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV-KTSPQTLSNLCL 563

  Fly   938 KINSKLNGINHKIKDDPRLPMMKN-TMYIGADVTHPSPDQREIPSVVGVAASHDPYGASYNMQYR 1001
            |||.||.|||:.:....|..:.:. .:::|||||||.....:.||:..|..|.|.:.:.|....|
Human   564 KINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVR 628

  Fly  1002 LQRGALEEIEDMFSITLEHL-RVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACDKV 1065
            :||...|.|||:..:..|.| :.||..| ..|..||:|||||.:||.|:|.:.||..|:.||.|:
Human   629 VQRPRQEIIEDLSYMVRELLIQFYKSTR-FKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKL 692

  Fly  1066 --GCKPKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGT 1128
              ..:|.|..::|.||||||.|.:.......|..|:..||.||..|.||.|..|::.||..||||
Human   693 EKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGT 757

  Fly  1129 AKPTRYNVIENTGNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRVYL 1186
            ::|:.|.|:.:......|.||.|||.|||.:.||.||||.|||||.|.|||.|.|.:|
Human   758 SRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHL 815

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
AGO2	NP_730054.1	PLN03202	411..1180	CDD:215631	266/806 (33%)
		ArgoN	411..543	CDD:293095	37/137 (27%)
		ArgoL1	554..601	CDD:285861	20/52 (38%)
		PAZ	605..719	CDD:239207	40/128 (31%)
		Piwi_ago-like	764..1186	CDD:240015	155/432 (36%)
AGO1	NP_001304051.1	PLN03202	26..856	CDD:215631	271/822 (33%)
		ArgoN	26..164	CDD:293095	38/145 (26%)
		ArgoL1	175..224	CDD:285861	21/54 (39%)
		PAZ_argonaute_like	225..345	CDD:239212	40/129 (31%)
		Piwi_ago-like	390..815	CDD:240015	155/432 (36%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1041
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG58336
OrthoDB	1	1.010	-	-		D159407at2759
OrthoFinder	1	1.000	-	-		FOG0000468
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR22891
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	6	5.930