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Protein Alignment AGO2 and Ago2

DIOPT Version :9

Sequence 1:	NP_730054.1	Gene:	AGO2 / 39683	FlyBaseID:	FBgn0087035	Length:	1217	Species:	Drosophila melanogaster
Sequence 2:	NP_694818.3	Gene:	Ago2 / 239528	MGIID:	2446632	Length:	860	Species:	Mus musculus

Alignment Length:	845	Identity:	273/845 - (32%)
Similarity:	422/845 - (49%)	Gaps:	70/845 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly   384 YQSRGPPQQQQA---APLP-----LPPQPAGSIKRGTIGKPGQVGINYLDLDLSKMPSVAYHYDV 440
            |...||.....|   :|:|     .||:|    ..||.|:..::..|:.::|:.|:.  .|||::
Mouse     2 YSGAGPVLASPAPTTSPIPGYAFKPPPRP----DFGTTGRTIKLQANFFEMDIPKID--IYHYEL 60

  Fly   441 KIMPER-PKKFYRQAFE----QFRVDQLGGAVLAYDGKASCYSVDKLPLNSQNPEVTVT-DRNGR 499
            .|.||: |::..|:..|    .|:....|.....:||:.:.|:...||:.....|:.|| ...|:
Mouse    61 DIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGK 125

  Fly   500 TLRYTIEIKETGDSTIDLKSLTTYMNDRIFDKPMRAMQCVEVVLASPCHNKAIR---VGRSFFKM 561
            ...:.:.||..  |.:.|::|...::.|:...|...:|.::||:.   |..::|   ||||||..
Mouse   126 DRIFKVSIKWV--SCVSLQALHDALSGRLPSVPFETIQALDVVMR---HLPSMRYTPVGRSFFTA 185

  Fly   562 SDPNNRHELDDGYEALVGLYQAFMLGDRP-----FLNVDISHKSFPISMPMIEYL-ERFSLKAKI 620
            |: ...:.|..|.|...|.:|:.    ||     .||:|:|..:|..:.|:||:: |....|:..
Mouse   186 SE-GCSNPLGGGREVWFGFHQSV----RPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIE 245

  Fly   621 NNTTNLDYSRR--FLEPFLRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETF---EHDGKKV- 679
            .....|..|:|  |.:. ::|:.|..|.....:   |.|||..::|.|||.:||   :..|:.|
Mouse   246 EQQKPLTDSQRVKFTKE-IKGLKVEITHCGQMK---RKYRVCNVTRRPASHQTFPLQQESGQTVE 306

  Fly   680 -TIASYFHSRN-YPLKFPQLHCLNVGSSIKSILLPIELCSIEEGQALNRKDGATQVANMIKYAAT 742
             |:|.||..|: ..|::|.|.||.||...|...||:|:|:|..||...:|....|.:.||:..|.
Mouse   307 CTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATAR 371

  Fly   743 STNVRKRKIMNLLQYFQHNLDPTISRFGIRIANDFIVVSTRVLSPPQVEY--HSKRFTMVKNGSW 805
            |...|:.:|..|::....|.||.:..|||.:.::...|:.|||.||.:.|  .:|.......|.|
Mouse   372 SAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVW 436

  Fly   806 RMDGMKF---LEPKPKAHKCAVLYCDPRSGRKMNYTQLNDFGNLIISQGKAVNISLDSDVTYRPF 867
            .|...:|   :|.|..|..|..    |:  |:.....|..|...:....:...:.:.....:..:
Mouse   437 DMRNKQFHTGIEIKVWAIACFA----PQ--RQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKY 495

  Fly   868 TDDERSLDTIFADLKRSQH--DLAIVIIPQFRISYDTIKQKAELQHGILTQCIKQFTVERKCNNQ 930
            .....|::.:|..||.:..  .|.:||:|.....|..:|:..:...|:.|||::...|:| ...|
Mouse   496 AQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQR-TTPQ 559

  Fly   931 TIGNILLKINSKLNGINHKIKDDPRLPMMKN-TMYIGADVTHPSPDQREIPSVVGVAASHDPYGA 994
            |:.|:.||||.||.|:|:.:....|.|:.:. .:::|||||||.....:.||:..|..|.|.:..
Mouse   560 TLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPN 624

  Fly   995 SYNMQYRLQRGALEEIEDMFSITLEHL-RVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCI 1058
            .|....|:|:...|.|:|:.::..|.| :.||..| ..|..||:||||||:|||.::.:.||..|
Mouse   625 RYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTR-FKPTRIIFYRDGVSEGQFQQVLHHELLAI 688

  Fly  1059 KQACDKV--GCKPKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVS 1121
            ::||.|:  ..:|.|..::|.||||||.|.:.......|..|:..||.||..|.||.|..|::.|
Mouse   689 REACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCS 753

  Fly  1122 HQAIQGTAKPTRYNVIENTGNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRVYL 1186
            |..||||::|:.|:|:.:......|.||.|||.|||.:.||.||||.|||||.|||||.|.|.:|
Mouse   754 HAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL 818

Fly 1187 1186
Mouse 819 818

Known Domains:

Indicated by green bases in alignment.

Software error:

Illegal division by zero at /www/www.flyrnai.org/docroot/cgi-bin/DRSC_prot_align.pl line 591.

For help, please send mail to the webmaster (ritg@hms.harvard.edu), giving this error message and the time and date of the error.