Protein Alignment AGO2 and Ago3

DIOPT Version :10

• Sequence 1: NP_730054.1
• Gene: AGO2 / 39683
• FlyBaseID: FBgn0087035
• Length: 1217
• Species: Drosophila melanogaster
• Sequence 2: NP_700451.2
• Gene: Ago3 / 214150
• MGIID: 2446634
• Length: 860
• Species: Mus musculus
• Alignment Length: 844
• Identity: 271/844 - (32%)
• Similarity: 416/844 - (49%)
• Gaps: 73/844 - (8%)

  Fly   386 SRGPPQQQQAAPLPLPPQPAGSIKRGTIGKPGQVGINYLDLDLSKMPSVAYHYDVKIMPER-PKK 449
            |.||...|   ||.:.|:..|   .||:|||.::..|...:::.|:.  .|.|:|.|.|:: |::
Mouse     5 SAGPIGAQ---PLFIVPRRPG---YGTMGKPIKLLANCFQVEIPKID--VYLYEVDIKPDKCPRR 61

  Fly   450 FYRQA----FEQFRVDQLGGAVLAYDGKASCYSVDKLPL--NSQNPEVTVTDRNGRTLRYTIEIK 508
            ..|:.    .:.|:|...|.....||||.|.|:.:.||:  ...:.:||:....|:...:.:.:|
Mouse    62 VNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSVK 126

  Fly   509 ETGDSTIDL--KSLT--TYMNDRIFDKPM--RAMQCVEVVLASPCHNKAIRVGRSFFKMSDPNNR 567
            .....:..|  ::|.  |.......|||:  ..:..|:|||......|...||||||...:..:
Mouse   127 FVSRVSWHLLHEALAGGTLPEPLELDKPVSTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYD- 190

  Fly   568 HELDDGYEALVGLYQAFMLGDRP-----FLNVDISHKSFPISMPMIEYLERFSLKAKINNTTNLD 627
            |.|..|.|...|.:|:.    ||     .||:|:|..:|..:.|:|:::      .::.:..|:|
Mouse   191 HPLGGGREVWFGFHQSV----RPAMWKMMLNIDVSATAFYKAQPVIQFM------CEVLDIHNID 245

  Fly   628 YSRRFLEPF--------LRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETFE---HDGKKV-- 679
            ...|.|...        ::|:.|..|...:.:   |.|||..::|.|||.:||.   .:|:.|
Mouse   246 EQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMR---RKYRVCNVTRRPASHQTFPLQLENGQTVER 307

  Fly   680 TIASYFHSR-NYPLKFPQLHCLNVGSSIKSILLPIELCSIEEGQALNRKDGATQVANMIKYAATS 743
            |:|.||..: ...||:|.|.||.||...|...||:|:|:|..||...:|....|.:.|||..|.|
Mouse   308 TVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARS 372

  Fly   744 TNVRKRKIMNLLQYFQHNLDPTISRFGIRIANDFIVVSTRVLSPPQVEYHSKRFTMV--KNGSWR 806
            ...|:.:|..|::...:..||.:..|..::.::...|:.|||..|.::|..:..|:.  .:|.|.
Mouse   373 APDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWD 437

  Fly   807 MDGMKF---LEPKPKAHKCAVLYCDPRSGRKMNYTQLNDFGNLIISQGKAVNISLDSDVTYRPFT 868
            |.|.:|   :|.|..|..|..      :.|:.....|..|.:.:....|...:.:.....:..:.
Mouse   438 MRGKQFHTGVEIKMWAIACFA------TQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYA 496

  Fly   869 DDERSLDTIFADLKR--SQHDLAIVIIPQFRISYDTIKQKAELQHGILTQCIKQFTVERKCNNQT 931
            ....|::.:|..||.  |...|.|||:|.....|..:|:..:...|:.|||::...| .|.:.||
Mouse   497 QGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNV-IKTSPQT 560

  Fly   932 IGNILLKINSKLNGINHKIKDDPRLPMMKN-TMYIGADVTHPSPDQREIPSVVGVAASHDPYGAS 995
            :.|:.||||.||.|||:.:....|..:.:. .:::|||||||.....:.||:..|..|.|.:.:.
Mouse   561 LSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSR 625

  Fly   996 YNMQYRLQRGALEEIEDMFSITLEHL-RVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIK 1059
            |....|:||...|.|:|:.|:..|.| :.||..| ..|..||:||||||:|||.::...||..|:
Mouse   626 YCATVRVQRPRQEIIQDLASMVRELLIQFYKSTR-FKPTRIIFYRDGVSEGQFRQVLYYELLAIR 689

  Fly  1060 QACDKV--GCKPKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSH 1122
            :||..:  ..:|.|..::|.||||||.|.:.......:..|:..||.||..|.||.|..|::.||
Mouse   690 EACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSH 754

  Fly  1123 QAIQGTAKPTRYNVIENTGNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRVYL 1186
            ..||||::|:.|:|:.:......|.||.|||.|||.:.||.||||.|||||.|||||.|.|.:|
Mouse   755 AGIQGTSRPSHYHVLWDDNFFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL 818

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO2	NP_730054.1	ArgoN	471..543	CDD:465134	19/79 (24%)
		ArgoL1	554..601	CDD:462567	18/51 (35%)
		PAZ	605..719	CDD:239207	38/127 (30%)
		Piwi_ago-like	764..1186	CDD:240015	151/432 (35%)
Ago3	NP_700451.2	ArgoN	73..167	CDD:465134	23/93 (25%)
		ArgoL1	177..227	CDD:462567	19/54 (35%)
		PAZ_argonaute_like	228..348	CDD:239212	38/128 (30%)
		Piwi_ago-like	393..818	CDD:240015	151/432 (35%)
		Interaction with guide RNA. /evidence=ECO:0000250|UniProtKB:Q9H9G7	530..567		11/37 (30%)
		Interaction with guide RNA. /evidence=ECO:0000250|UniProtKB:Q9H9G7	758..805		24/46 (52%)