Protein Alignment AGO2 and AGO4

DIOPT Version :10

• Sequence 1: NP_730054.1
• Gene: AGO2 / 39683
• FlyBaseID: FBgn0087035
• Length: 1217
• Species: Drosophila melanogaster
• Sequence 2: NP_060099.2
• Gene: AGO4 / 192670
• HGNCID: 18424
• Length: 861
• Species: Homo sapiens
• Alignment Length: 847
• Identity: 272/847 - (32%)
• Similarity: 416/847 - (49%)
• Gaps: 82/847 - (9%)

  Fly   388 GPPQQQQAAPLPLPPQPAGSIKRGTIGKPGQVGINYLDLDLSKMPSVAYHYDVKIMPE-RPKKFY 451
            |||     |.|..||:..|   .||:|||.::..|:..:.:.|:.  .|||||.|.|| ||::..
Human     7 GPP-----ASLFQPPRRPG---LGTVGKPIRLLANHFQVQIPKID--VYHYDVDIKPEKRPRRVN 61

  Fly   452 RQAFE----QFRVDQLGGAVLAYDGKASCYSVDKLPLNSQNPEVTVT-DRNGRTLRYTIEIKETG 511
            |:..:    .|::...|.....||||.:.|:...||:.....::.|| ...|:...:.:.::..
Human    62 REVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWV- 125

  Fly   512 DSTIDLKSLTTYMNDRIFDKPMRAMQCVEVVLASPCHNKAIR---VGRSFFKMSDPNN-RHELDD 572
             |.:.|:.|...:...:.:.|..::|.::|:..   |..::|   ||||||  |.|.. .|.|..
Human   126 -SVVSLQLLLEALAGHLNEVPDDSVQALDVITR---HLPSMRYTPVGRSFF--SPPEGYYHPLGG 184

  Fly   573 GYEALVGLYQAFMLGDRP-----FLNVDISHKSFPISMPMIEYLERFSLKAKINNTTN--LDYSR 630
            |.|...|.:|:.    ||     .||:|:|..:|..:.|:||::........||..|.  .|..|
Human   185 GREVWFGFHQSV----RPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQR 245

  Fly   631 RFLEPFLRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETFE---HDGK--KVTIASYFHSR-N 689
            ......:||:.|..|.....:   |.|||..::|.|||.:||.   .:|:  :.|:|.||..: :
Human   246 VKFTKEIRGLKVEVTHCGQMK---RKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYS 307

  Fly   690 YPLKFPQLHCLNVGSSIKSILLPIELCSIEEGQALNRKDGATQVANMIKYAATSTNVRKRKIMNL 754
            ..||:|.|.||.||...|...||:|:|:|..||...:|....|.:.|||..|.|...|:.:|..|
Human   308 LQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRL 372

  Fly   755 LQYFQHNL----DPTISRFGIRIANDFIVVSTRVLSPPQVEYHSKRFTMV--KNGSWRMDGMKF- 812
            ::  .:::    ||.:..|||.:.|:...::.|||..|.::|..:..|:.  ..|.|.|.|.:|
Human   373 VK--SNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFY 435

  Fly   813 --LEPKPKAHKCAVLYCDPRSGRKMNYTQLNDFGNLIISQGKAVNISLDSDVTYRPFTDDERSLD 875
              :|.|..|..|..    |:  ::.....|..|.:.:....|...:.:.....:..:.....|::
Human   436 AGIEIKVWAVACFA----PQ--KQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVE 494

  Fly   876 TIFADLKRSQ--HDLAIVIIPQFRISYDTIKQKAELQHGILTQCIKQFTVERKCNNQTIGNILLK 938
            .:|..||.:.  ..|.:||:|.....|..:|:..:...|:.|||::...|. |.:.||:.|:.||
Human   495 PMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV-KTSPQTLSNLCLK 558

  Fly   939 INSKLNGINHKIKDDPRLPMMKN-TMYIGADVTHPSPDQREIPSVVGVAASHDPYGASYNMQYRL 1002
            ||:||.|||:.:....|..:.:. .:::|||||||.....:.||:..|..|.|.:.:.|....|:
Human   559 INAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRV 623

  Fly  1003 QRG----------ALEEIEDMFSITLEHL-RVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELR 1056
            |..          :.|.|:|:.::..|.| :.||..| ..|..|||||.|||:||..::...||.
Human   624 QTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTR-FKPTRIIYYRGGVSEGQMKQVAWPELI 687

  Fly  1057 CIKQACDKV--GCKPKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFM 1119
            .|::||..:  ..:|.|..::|.||||||.|.:.......|..||..||.||.||.||:|..|::
Human   688 AIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYL 752

  Fly  1120 VSHQAIQGTAKPTRYNVIENTGNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRV 1184
            .||..||||::|:.|.|:.:......|.||.|||.|||.:.||.||||.|||||.|.|||.|.|.
Human   753 CSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARY 817

  Fly  1185 YL 1186
            :|
Human   818 HL 819

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO2	NP_730054.1	ArgoN	471..543	CDD:465134	15/72 (21%)
		ArgoL1	554..601	CDD:462567	20/52 (38%)
		PAZ	605..719	CDD:239207	40/121 (33%)
		Piwi_ago-like	764..1186	CDD:240015	150/442 (34%)
AGO4	NP_060099.2	ArgoN	26..155	CDD:465134	32/132 (24%)
		ArgoL1	166..216	CDD:462567	21/55 (38%)
		PAZ_argonaute_like	217..337	CDD:239212	40/122 (33%)
		Piwi_ago-like	384..819	CDD:240015	150/442 (34%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	825..846