Protein Alignment Best4 and Best3

DIOPT Version :9

• Sequence 1: NP_001097609.1
• Gene: Best4 / 39660
• FlyBaseID: FBgn0036491
• Length: 526
• Species: Drosophila melanogaster
• Sequence 2: NP_001007584.1
• Gene: Best3 / 382427
• MGIID: 3580298
• Length: 669
• Species: Mus musculus
• Alignment Length: 575
• Identity: 211/575 - (36%)
• Similarity: 312/575 - (54%)
• Gaps: 78/575 - (13%)

  Fly     1 MTVTYTSRVSTSSVLSNFLGLLVRWRGSIYKLIWLDLIVFFGLYCLLAITYRLLVNEEGKRFFEA 65
            |||||:|:|:.::.. .|..||::||||||||::.:.|||..||..:::.||||:....||:||.
Mouse     1 MTVTYSSKVANATFF-GFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGAQKRYFEK 64

  Fly    66 IMSYCEKNGSLIPLSFVLGFFVRIVMNRWWDQYTTIPWPDGIAILISTSIHGSDDRARVMRRTIL 130
            :..||::....||::|||||:|.:|:||||:|:..:||||.:.:|||:|:||||...|::|||::
Mouse    65 LSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMLLISSSVHGSDQHGRLLRRTLM 129

  Fly   131 RYVCLCQVIVFTMISPRVKRRFPTYTQIIEAGFLLENEKKIIEAMDQAFPSYPKHWMPIVWAASI 195
            |||.|..:::|..:|..|.:||||...::||||:..:|:|:   .|.....:.|:|:|.:|..::
Mouse   130 RYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTADERKL---FDHLKSPHLKYWVPFIWFGNL 191

  Fly   196 VMRARRENKIRDDYAVKTIIDELNQLRGNCGFLLYYDWISVPLVYTQVVTVATYSFFLFSVLGQQ 260
            ..:||.|.:|||...:::::.|:|:.|..|..|..|||:.:|||||||||:|.|:||...::|:|
Mouse   192 ATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQ 256

  Fly   261 WNESHSDEDGTRIRRWFPILTVLQFFFYMGWLKVAETLINPFGEDDDDFELNWIIDRNLTVSYCI 325
            :.:......|..:..:.||.|:||||||.|||||||.|||||||||||||.||.|||||.||...
Mouse   257 FLDPTKGYVGHDLDLYVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLA 321

  Fly   326 VDEMHQEHPELVKDQYWEEVFPNEIPYAQPKMRQNPPA--ASTAYM------------------- 369
            |||||...|::.||.||::..... ||.........|:  .||..|
Mouse   322 VDEMHMSLPKMKKDIYWDDSAARP-PYTLAAADYCIPSFLGSTIQMGLSGSNFPAEDWLWNYEKH 385

  Fly   370 ESQSKLQKRHESAMSTL----STRSRNNFRQNFLGRPSATGSY----SDSIQMEDGPSRVTFSEN 426
            .::..:.:|.:..:||.    |.|.|.:|     ||.::..|.    |.:..:.|.|||
Mouse   386 GNRHSVMRRVKRFLSTHEHPGSPRRRRSF-----GRQASDSSMFLPPSPARDLLDVPSR------ 439

  Fly   427 QFDLPHDEANSSHKPSQST-------IDFNDLIEQRRRERRERIRHRFLDMQSTHLRHDTSGAPY 484
               .|| ..:.:.|.|:|.       ....:|...|...|...::.  |..||: :|...:..|.
Mouse   440 ---NPH-RGSPTRKQSRSQEGSPKLHSSMGELSTIRETSRTSTLQS--LSPQSS-VRSSPTKMPQ 497

  Fly   485 T----ITVLRPPS--------DETLS-------ESQEAGDRAQQDPSGSKEPPPN 520
            .    ||....|:        |.|.|       ..|.:|...|.:|||:....||
Mouse   498 VPEVLITAAEAPAFSADSHQHDSTTSILSLEFTGVQPSGTEQQVEPSGTPPGDPN 552

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Best4	NP_001097609.1	Bestrophin	8..319	CDD:279411	143/310 (46%)
Best3	NP_001007584.1	Bestrophin	8..315	CDD:279411	143/310 (46%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	399..496		26/114 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	533..560		8/20 (40%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	591..627
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	646..669

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167845754
Domainoid	1	1.000	355	1.000	Domainoid score	I981
eggNOG	1	0.900	-	-		E1_KOG3547
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	396	1.000	Inparanoid score	I1945
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D145964at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm8695
orthoMCL	1	0.900	-	-		OOG6_101870
Panther	1	1.100	-	-	O	PTHR10736
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2974
SonicParanoid	1	1.000	-	-		X320
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.790