Protein Alignment Best4 and BEST3

DIOPT Version :9

• Sequence 1: NP_001097609.1
• Gene: Best4 / 39660
• FlyBaseID: FBgn0036491
• Length: 526
• Species: Drosophila melanogaster
• Sequence 2: NP_116124.2
• Gene: BEST3 / 144453
• HGNCID: 17105
• Length: 668
• Species: Homo sapiens
• Alignment Length: 594
• Identity: 210/594 - (35%)
• Similarity: 308/594 - (51%)
• Gaps: 98/594 - (16%)

  Fly     1 MTVTYTSRVSTSSVLSNFLGLLVRWRGSIYKLIWLDLIVFFGLYCLLAITYRLLVNEEGKRFFEA 65
            |||||:|:|:.::.. .|..||::||||||||::.:.|||..||..:::.||||:....||:||.
Human     1 MTVTYSSKVANATFF-GFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGVQKRYFEK 64

  Fly    66 IMSYCEKNGSLIPLSFVLGFFVRIVMNRWWDQYTTIPWPDGIAILISTSIHGSDDRARVMRRTIL 130
            :..||::....||::|||||:|.:|:||||:|:..:||||.:..|||:|:||||:..|::|||::
Human    65 LSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDEHGRLLRRTLM 129

  Fly   131 RYVCLCQVIVFTMISPRVKRRFPTYTQIIEAGFLLENEKKIIEAMDQAFPSYPKHWMPIVWAASI 195
            |||.|..:::|..:|..|.:||||...::||||:..:|:|:...:..   .:.|:|:|.:|..::
Human   130 RYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTTDERKLFNHLKS---PHLKYWVPFIWFGNL 191

  Fly   196 VMRARRENKIRDDYAVKTIIDELNQLRGNCGFLLYYDWISVPLVYTQVVTVATYSFFLFSVLGQQ 260
            ..:||.|.:|||...:::::.|:|:.|..|..|..|||:.:|||||||||:|.|:||...::|:|
Human   192 ATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQ 256

  Fly   261 WNESHSDEDGTRIRRWFPILTVLQFFFYMGWLKVAETLINPFGEDDDDFELNWIIDRNLTVSYCI 325
            :.:......|..:..:.||.|:||||||.|||||||.|||||||||||||.||.|||||.||...
Human   257 FLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLA 321

  Fly   326 VDEMHQEHPELVKDQYWEEVFPNEIPYAQPKMRQNPPA--ASTAYM------------------- 369
            |||||...|::.||.||::..... ||.........|:  .||..|
Human   322 VDEMHMSLPKMKKDIYWDDSAARP-PYTLAAADYCIPSFLGSTVQMGLSGSDFPDEEWLWDYEKH 385

  Fly   370 ---ESQSKLQKRHESAMSTLSTRSRNNFRQN------FLGR-----------------PSATGSY 408
               .|..:..||..||....|:..|.::|:.      ||.|                 |.|:.::
Human   386 GHRHSMIRRVKRFLSAHEHPSSPRRRSYRRQTSDSSMFLPRDDLSPARDLLDVPSRNPPRASPTW 450

  Fly   409 SDSIQMEDGPSRVTFSENQFDLPHDEANSSHKPS---QSTIDFNDLIEQRRRERRERIRHRFLD- 469
            ..|...|..|: :.||..:.....:.:.:|...|   ||::            |...|:...:.
Human   451 KKSCFPEGSPT-LHFSMGELSTIRETSQTSTLQSLTPQSSV------------RTSPIKMPLVPE 502

  Fly   470 ----------MQSTHLRHD--TSGAPYTITVLRPPSDETLSESQEAGDRAQQDPSGS------KE 516
                      ..|....||  ||......|.::|...|           .||.|.||      ||
Human   503 VLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTE-----------QQQGPMGSILSPSEKE 556

  Fly   517 PPPNKTEKQ 525
            .||.....|
Human   557 TPPGGPSPQ 565

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Best4	NP_001097609.1	Bestrophin	8..319	CDD:279411	142/310 (46%)
BEST3	NP_116124.2	Bestrophin	8..315	CDD:307280	142/310 (46%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	400..454		10/53 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	473..493		5/31 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	532..570		13/45 (29%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165155285
Domainoid	1	1.000	355	1.000	Domainoid score	I996
eggNOG	1	0.900	-	-		E1_KOG3547
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	396	1.000	Inparanoid score	I1965
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D145964at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm8455
orthoMCL	1	0.900	-	-		OOG6_101870
Panther	1	1.100	-	-	O	PTHR10736
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2974
SonicParanoid	1	1.000	-	-		X320
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	13	12.790