Protein Alignment Best4 and best3

DIOPT Version :9

• Sequence 1: NP_001097609.1
• Gene: Best4 / 39660
• FlyBaseID: FBgn0036491
• Length: 526
• Species: Drosophila melanogaster
• Sequence 2: NP_001155224.1
• Gene: best3 / 100302039
• XenbaseID: XB-GENE-1194414
• Length: 657
• Species: Xenopus tropicalis
• Alignment Length: 569
• Identity: 201/569 - (35%)
• Similarity: 316/569 - (55%)
• Gaps: 59/569 - (10%)

  Fly     1 MTVTYTSRVSTSSVLSNFLGLLVRWRGSIYKLIWLDLIVFFGLYCLLAITYRLLVNEEGKRFFEA 65
            |||||:|:|:.::.. .|..||::||||||||::.:.::|..||..|::.||..:.:..||:||.
 Frog     1 MTVTYSSKVANATFF-GFHRLLLKWRGSIYKLLYREFLLFTSLYTTLSVIYRFFLTDSQKRYFEK 64

  Fly    66 IMSYCEKNGSLIPLSFVLGFFVRIVMNRWWDQYTTIPWPDGIAILISTSIHGSDDRARVMRRTIL 130
            :..||:|....||::|||||:|.:|:||||:|:..:||||.:..|||:::||.|:..|::|||::
 Frog    65 VSMYCDKYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSNVHGRDEYGRLLRRTLM 129

  Fly   131 RYVCLCQVIVFTMISPRVKRRFPTYTQIIEAGFLLENEKKIIEAMDQAFPSYPKHWMPIVWAASI 195
            |||.|..:::|..:|..|.:||||...::||||:..:|:|:.:::..   .:.|:|:|::|..::
 Frog   130 RYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTSDERKLFDSLKS---PHLKYWVPVIWFGNL 191

  Fly   196 VMRARRENKIRDDYAVKTIIDELNQLRGNCGFLLYYDWISVPLVYTQVVTVATYSFFLFSVLGQQ 260
            ..:||.|.:|||...::.:::|:|:.|..|..|..|||:.:|||||||||:|.|:||...::|:|
 Frog   192 ASKARSEGRIRDSIDLQMMMNEMNKFRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACIIGRQ 256

  Fly   261 WNESHSDEDGTRIRRWFPILTVLQFFFYMGWLKVAETLINPFGEDDDDFELNWIIDRNLTVSYCI 325
            :.:......|..:..:.|:.|:||||||.|||||||.|||||||||||||.||.|||||.||...
 Frog   257 FLDPKQAYPGHDLDLYIPVFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLMA 321

  Fly   326 VDEMHQEHPELVKDQYW--EEVFPNEIPYAQPKMRQNPPA--ASTAYMESQSKLQKRHESAMSTL 386
            |||||...|::.:|.||  .:|.|   ||.........|:  .||.:|.....:..|.:..:...
 Frog   322 VDEMHMNLPKMNRDIYWNDSDVRP---PYTLAAADYCIPSFLGSTVHMGLPDSVFLREDWLLDED 383

  Fly   387 STRSRNNFR---QNFLG------RPSATGSYSDSIQMEDG-----PSRVTFSENQFDLPH----- 432
            ..|.:::..   :.||.      ..|:.|.||.  |..|.     ||.:.:|.:..|.||
 Frog   384 KPRRQHSVLRRVKRFLSVHEHSPDSSSRGGYSR--QGSDASNIFFPSELNYSGSFHDAPHRARHH 446

  Fly   433 -------DEANSSHKPSQSTIDFNDLIEQRRRERRERIRHRFLDMQSTHLRHD---------TSG 481
                   :..:.:.||:....|.:.:.|..|.:...|.      ..|..|..|         ||.
 Frog   447 AFHTKRRESTDKNSKPTTPKGDLSTIREASRSDTSGRA------STSNGLTPDIALKVPEVATSS 505

  Fly   482 APYTITVLR---PPSDE--TLSESQEAGDRAQQDPSGSKEPPPNKTEKQ 525
            :..|:|...   ||...  |:|::.|.....|.|.:.:.:.|...::::
 Frog   506 SGETMTTAHSAVPPQSNSLTVSDTMECSGNYQCDTNTAVQHPEQVSQEE 554

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Best4	NP_001097609.1	Bestrophin	8..319	CDD:279411	137/310 (44%)
best3	NP_001155224.1	Bestrophin	8..316	CDD:395843	138/311 (44%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	355	1.000	Domainoid score	I987
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	377	1.000	Inparanoid score	I2034
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D145964at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm9346
Panther	1	1.100	-	-	O	PTHR10736
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X320
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	7	7.160