Protein Alignment Best4 and best4

DIOPT Version :9

• Sequence 1: NP_001097609.1
• Gene: Best4 / 39660
• FlyBaseID: FBgn0036491
• Length: 526
• Species: Drosophila melanogaster
• Sequence 2: NP_001116914.1
• Gene: best4 / 100144681
• XenbaseID: XB-GENE-1194400
• Length: 514
• Species: Xenopus tropicalis
• Alignment Length: 531
• Identity: 215/531 - (40%)
• Similarity: 306/531 - (57%)
• Gaps: 47/531 - (8%)

  Fly     1 MTVTYTSRVSTSSVLSNFLGLLVRWRGSIYKLIWLDLIVFFGLYCLLAITYRLLVNEEGKRFFEA 65
            |||:||.:|:.:| ...|..||.||:||||||::.:.|||..||.:|::|||.|:||:.|..|..
 Frog     1 MTVSYTLKVANAS-FGCFFKLLFRWKGSIYKLLYKEFIVFCCLYAMLSVTYRCLLNEKQKSEFAQ 64

  Fly    66 IMSYCEKNGSLIPLSFVLGFFVRIVMNRWWDQYTTIPWPDGIAILISTSIHGSDDRARVMRRTIL 130
            :..||.:...|||:||||||:|.:::||||.|||.||.||.:..::|:::||.|:|.|::|||::
 Frog    65 LARYCNQYTDLIPVSFVLGFYVTLIVNRWWAQYTNIPLPDQLMCVVSSNVHGGDERGRLLRRTMM 129

  Fly   131 RYVCLCQVIVFTMISPRVKRRFPTYTQIIEAGFLLENEKKIIEAMDQAFPSYPKHWMPIVWAASI 195
            ||..|..|::...:|.||.:||||...|:||||:...|.|..|.:   |..|.|:|:|.||..::
 Frog   130 RYANLSSVLILRSVSTRVLKRFPTMEHIVEAGFMTREELKKYECL---FSDYNKYWIPCVWFTNL 191

  Fly   196 VMRARRENKIRDDYAVKTIIDELNQLRGNCGFLLYYDWISVPLVYTQVVTVATYSFFLFSVLGQQ 260
            ..:|||:.::|||.|:|.::||||:.|..|..|.:|||||||||||||||:|.||||...::|:|
 Frog   192 AAQARRDGRVRDDVALKLLMDELNKYRAKCSMLFHYDWISVPLVYTQVVTIAVYSFFGVCIIGRQ 256

  Fly   261 WNESHSDEDGTRIRRWFPILTVLQFFFYMGWLKVAETLINPFGEDDDDFELNWIIDRNLTVSYCI 325
            :.|...:|:...|..:.||.|:||||||.||||||:.:||||||||||||.|.:|||||.||...
 Frog   257 FLEPELEEEKPNIDLYVPIFTLLQFFFYSGWLKVAQQIINPFGEDDDDFETNRLIDRNLQVSLLS 321

  Fly   326 VDEMHQEHPELVKDQYWEE--VFPNEIPY--AQPKMRQNPPAASTAY-MESQSKLQKRHESAMST 385
            ||:|:|..|.|.||:||::  ..|   ||  |.......|....:|: |......::..:.|::.
 Frog   322 VDDMYQNLPPLEKDKYWDDQTAAP---PYTLATAAETLKPSFMGSAFDMRFLGDAEQSQQVAVTP 383

  Fly   386 LSTRSRNNF--------------RQNFLGRPSATGSYSDSIQMEDGPSRVTFSENQFDLPHDEAN 436
            ...|:|...              .:||:|| ...|.:....:.:..||....|.|. ||.||
 Frog   384 NMPRARTPMLSRFLSSAPSPAVSLKNFMGR-GTRGHHLVRFRGDGYPSPNPNSRNS-DLEHD--- 443

  Fly   437 SSHKPSQSTIDFNDLIEQRRRERRE---------RIRHRFLDMQSTHLRHDTSGAPYTITVLRPP 492
                 |...||..:..|..:..|..         |:..| |:......:.:....|..:.::..|
 Frog   444 -----SVGRIDEEETTEDEKESRASEPPTPKVTLRVPRR-LEASEQQKKREQPKPPPLVIIVDEP 502

  Fly   493 SDETL-SESQE 502
            |||:| ||..|
 Frog   503 SDESLNSEPAE 513

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Best4	NP_001097609.1	Bestrophin	8..319	CDD:279411	156/310 (50%)
best4	NP_001116914.1	Bestrophin	8..315	CDD:279411	156/310 (50%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	355	1.000	Domainoid score	I987
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H129743
Inparanoid	1	1.050	377	1.000	Inparanoid score	I2034
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D145964at33208
OrthoFinder	1	1.000	-	-		FOG0001515
OrthoInspector	1	1.000	-	-		mtm9346
Panther	1	1.100	-	-	O	PTHR10736
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2974
SonicParanoid	1	1.000	-	-		X320
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	11	11.100