DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Msh6 and MSH6

DIOPT Version :9

Sequence 1:	NP_648755.1	Gene:	Msh6 / 39654	FlyBaseID:	FBgn0036486	Length:	1190	Species:	Drosophila melanogaster
Sequence 2:	NP_010382.3	Gene:	MSH6 / 851671	SGDID:	S000002504	Length:	1242	Species:	Saccharomyces cerevisiae

Alignment Length:	1317	Identity:	364/1317 - (27%)
Similarity:	593/1317 - (45%)	Gaps:	260/1317 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 SKKLNTSVGGTPTNTLLNYFSKSPAFDKK-KLTPSVKTDPDASKSEK-------ENLQNQQPKV- 57
            |||:......|..||..:..:|:|...|. ||...|..|.|.:.:|:       :.:.:|:|:.
Yeast    44 SKKVQKPTPATLENTATDKITKNPQGGKTGKLFVDVDEDNDLTIAEETVSTVRSDIMHSQEPQSD 108

  Fly    58 ----------------KDGKKEASKPAAKRKLPI--SDDEPA----SGQRKRKRIVQPESDSE-- 98
                            :|.....|:...||::..  |||:.:    :.:||:.::|..|||.:
Yeast   109 TMLNSNTTEPKSTTTDEDLSSSQSRRNHKRRVNYAESDDDDSDTTFTAKRKKGKVVDSESDEDEY 173

  Fly    99 -PEMEVTKSEDDFSDCASDYEPD--ENEASDDSVSSGAEEVSP---SENDMSVDSPTPK-----K 152
             |:......:||.:|...|.:.:  |:...||.:.|.||..|.   |.|.....||..:     .
Yeast   174 LPDKNDGDEDDDIADDKEDIKGELAEDSGDDDDLISLAETTSKKKFSYNTSHSSSPFTRNISRDN 238

  Fly   153 SRKKSKILN------NNNNNEPSSKKVKLESTIQLAEGATFQEKLKNLQSNAKQDASYDDIVTNT 211
            |:|||:...      |.::::||:..                   |:.:.|.:.:..|..:|
Yeast   239 SKKKSRPNQAPSRSYNPSHSQPSATS-------------------KSSKFNKQNEERYQWLV--- 281

  Fly   212 SNLDEPVVWPHQKLEFLQPDKIKDKEGRRPDHPDYDKSTLHVPEKFLNGLSPGVRQWWVLKSDNY 276
               ||                 :|.:.|....|:||..||::|....|..:|..:|:|.:||..:
Yeast   282 ---DE-----------------RDAQRRPKSDPEYDPRTLYIPSSAWNKFTPFEKQYWEIKSKMW 326

  Fly   277 DCVLFFKVGKFYELYHMDA-------DVGVNELGFTYMRGEFAHSGFPEISFDKMSTILVDRGFK 334
            ||::|||.|||:|||..||       |:.:...|    |.....:|.||:||:..:...:..|:|
Yeast   327 DCIVFFKKGKFFELYEKDALLANALFDLKIAGGG----RANMQLAGIPEMSFEYWAAQFIQMGYK 387

  Fly   335 VARVEQTETPDMMTERCKRIKATKFDKVVAREICQITNRGTQVFGSQCKIGPNHQPNYMLAIVEK 399
            ||:|:|.|:  |:.:..:  :.:|  .:|.||:..|...||...|....   :....:.|||.|:
Yeast   388 VAKVDQRES--MLAKEMR--EGSK--GIVKRELQCILTSGTLTDGDMLH---SDLATFCLAIREE 443

  Fly   400 ----------DEGTCSR------YGVCFIDTSIGDFHLGEFEDDKNCSRLLTLVSHHMPVLFLNE 448
                      |..|..:      :|..||||:.|:..:.|||||..|::|.||:|...|:..:.|
Yeast   444 PGNFYNETQLDSSTIVQKLNTKIFGAAFIDTATGELQMLEFEDDSECTKLDTLMSQVRPMEVVME 508

  Fly   449 KSALSQRTQQIVR--TVLGGILKEPVPGNGKHACSAEKT-LKLLAERYYAGPGSDDNWPLVLRTM 510
            ::.||....:||:  :....|..|...|...:.|  :|| .::::..|::   ::::||.||::.
Yeast   509 RNNLSTLANKIVKFNSAPNAIFNEVKAGEEFYDC--DKTYAEIISSEYFS---TEEDWPEVLKSY 568

  Fly   511 QSDMDHLGLTPNDNYKLALKALGQCIFFIHKCKLEPKVLPMARYQLYVPPDQLADAKPAVASTLR 575
            .          :...|:...|.|..::::...||:..::.|...:.|              ..::
Yeast   569 Y----------DTGKKVGFSAFGGLLYYLKWLKLDKNLISMKNIKEY--------------DFVK 609

  Fly   576 RSH-MVLDATTLSNLRII------GEEHSLLSTLDHCCTKFGKRLLHHWLCAPSCDVSVIKERQD 633
            ..| ||||..||.||.|.      .::.:|....:...|..|||::..||..|....:.|:.|.|
Yeast   610 SQHSMVLDGITLQNLEIFSNSFDGSDKGTLFKLFNRAITPMGKRMMKKWLMHPLLRKNDIESRLD 674

  Fly   634 AIGELIRMPTELQEVRALLAPMPDFERNLAQIHLFGNKQIKQMDHPDSRAILFEEKLYNKQKLQG 698
            ::..|::..|..:::....:.:||.||.||:||              ||.|          |::.
Yeast   675 SVDSLLQDITLREQLEITFSKLPDLERMLARIH--------------SRTI----------KVKD 715

  Fly   699 FMAVLKGFNDLTKLPTMF--HQCKTTLLKRITQLPESGGSFPDLSKELQYFATAFDHDAAAKTGV 761
            |..|:..|..:.:|....  :..|..:.|.|:..||.      |.:.::.:..||:...|....:
Yeast   716 FEKVITAFETIIELQDSLKNNDLKGDVSKYISSFPEG------LVEAVKSWTNAFERQKAINENI 774

  Fly   762 IAPQAGMDAEYDAAMDSIGEVEKRLKTYLVEQERHFGC-RITYFGSDKKRYQLDVPESHASKANK 825
            |.||.|.|.|:|.:||.|.|:|..|...|:...:.|.| .|.|..|.|:.|.:::|.|.......
Yeast   775 IVPQRGFDIEFDKSMDRIQELEDELMEILMTYRKQFKCSNIQYKDSGKEIYTIEIPISATKNVPS 839

  Fly   826 SYTLEGQTKGKKPSRRYTTAETRALLKDMQHAEDTRNMVLKDLARRLFEKFSNHYDQ-WKQCIDC 889
            ::.   |....|..:||.:.|.|||.:.|..|::....:.:||..||.:||..||:. |...|..
Yeast   840 NWV---QMAANKTYKRYYSDEVRALARSMAEAKEIHKTLEEDLKNRLCQKFDAHYNTIWMPTIQA 901

  Fly   890 VANLDVLGSL---AEYAGQQMVICVPELVSDADQP-------FIQLEEGYHPCAN-----ASTYI 939
            ::|:|.|.::   :||.|...  |.|.:|.:.|..       |::.:...|||.|     |..:|
Yeast   902 ISNIDCLLAITRTSEYLGAPS--CRPTIVDEVDSKTNTQLNGFLKFKSLRHPCFNLGATTAKDFI 964

  Fly   940 PNGLELGTASEAP-LSLLTGPNMGGKSTLMREVGLLVIMAQIGAHIPAASCRLSLVDRIFTRLGA 1003
            ||.:|||  .|.| |.||||.|..||||::|...:.|||||:|.::|..|..|:.:|||.|||||
Yeast   965 PNDIELG--KEQPRLGLLTGANAAGKSTILRMACIAVIMAQMGCYVPCESAVLTPIDRIMTRLGA 1027

  Fly  1004 QDDILAGHSTFLVELNETSLILKHATCHSLVLLDELGRGTATYDGTAIAASVVNFLA-NLKCRTL 1067
            .|:|:.|.|||.|||.||..||..||..||:::||||||.::.||.|||.||::.:| :::....
Yeast  1028 NDNIMQGKSTFFVELAETKKILDMATNRSLLVVDELGRGGSSSDGFAIAESVLHHVATHIQSLGF 1092

  Fly  1068 FSTHYHNLIDFFHNDKRITLGHMACMVENEDNADPTQETVTFLYKYTAGACPKSYGFNAAKLAGM 1132
            |:|||..|...|.:..::....|:.:|      |.....||||||...|....|:|.:.|.:.|:
Yeast  1093 FATHYGTLASSFKHHPQVRPLKMSILV------DEATRNVTFLYKMLEGQSEGSFGMHVASMCGI 1151

  Fly  1133 PQGIIKRAYELSKKVE---AIALQRKITA-----KIVAATAG----------NEDTKKEKI---- 1175
            .:.||..|...:..:|   .:..:|.:.|     ::|:...|          .:..|..|:
Yeast  1152 SKEIIDNAQIAADNLEHTSRLVKERDLAANNLNGEVVSVPGGLQSDFVRIAYGDGLKNTKLGSGE 1216

  Fly  1176 -------NALKDLLKQL 1185
                   |..:::||.|
Yeast  1217 GVLNYDWNIKRNVLKSL 1233

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Msh6	NP_648755.1	MttA_Hcf106	<44..156	CDD:294511	33/154 (21%)
		MutS	261..1186	CDD:223327	296/1008 (29%)
		MutS_I	263..375	CDD:279898	39/118 (33%)
		MutS_II	391..508	CDD:282973	38/135 (28%)
		MutS_III	584..>667	CDD:282977	25/88 (28%)
		MutS_IV	767..859	CDD:282975	30/92 (33%)
		ABC_MSH6_euk	924..1140	CDD:213253	94/222 (42%)
MSH6	NP_010382.3	PRK08581	71..>282	CDD:236304	49/235 (21%)
MSH6	NP_010382.3	MutS	306..1235	CDD:223327	297/1013 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157346179
Domainoid	1	1.000	119	1.000	Domainoid score	I1265
eggNOG	1	0.900	-	-		E1_COG0249
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H149
Inparanoid	1	1.050	409	1.000	Inparanoid score	I305
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53698
OrthoFinder	1	1.000	-	-		FOG0004305
OrthoInspector	1	1.000	-	-		oto100109
orthoMCL	1	0.900	-	-		OOG6_100652
Panther	1	1.100	-	-	LDO	PTHR11361
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R750
SonicParanoid	1	1.000	-	-		X3037
TreeFam	1	0.960	-	-
	15	14.790