DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pdi and Pdia3

DIOPT Version :9

Sequence 1:	NP_001287070.1	Gene:	Pdi / 39651	FlyBaseID:	FBgn0286818	Length:	496	Species:	Drosophila melanogaster
Sequence 2:	NP_059015.2	Gene:	Pdia3 / 29468	RGDID:	68430	Length:	510	Species:	Rattus norvegicus

Alignment Length:	538	Identity:	175/538 - (32%)
Similarity:	276/538 - (51%)	Gaps:	81/538 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MKFLICALFLAASYVAAS---AEAEVKVEEGVLVATVDNFKQLIADN---EFVLVEFYAPWCGHC 59
            |:|...||....:.:.||   |.|.:.....||..|.:||:..::|.   ..:||||:|||||||
  Rat     1 MRFSCLALLPGVALLLASALLASALLASASDVLELTDENFESRVSDTGSAGLMLVEFFAPWCGHC 65

  Fly    60 KALAPEYAKAAQQLAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSGSPV-EYSGGRQAA 123
            |.|||||..||.:|   :..:.|||||.|.......:|.|.||||||.||.|... .|.|.|.|.
  Rat    66 KRLAPEYEAAATRL---KGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTAD 127

  Fly   124 DIIAWVTKKTGPPAKDLTSVADAEQFLKDNEIAIIGFFKDLESEEAKTFTKVA-NALDSFVFGVS 187
            .|::.:.|:.||.:..|.:..:.::|:.|.:.:::|||:||.|:....|.|.| |..|::.|..:
  Rat   128 GIVSHLKKQAGPASVPLRTEDEFKKFISDKDASVVGFFRDLFSDGHSEFLKAASNLRDNYRFAHT 192

  Fly   188 SNADVIAKYEAKDNGVVLFKP------FDDKKSVF-EGELNEENLKKFAQ--------------- 230
            :...::.:|:....|:.:|:|      |:||...: |.::....:|||.|
  Rat   193 NVESLVKEYDDNGEGITIFRPLHLANKFEDKIVAYTEKKMTSGKIKKFIQESIFGLCPHMTEDNK 257

  Fly   231 --VQSLPLIV---DFNHESASKIFGGSIKSHLLFFVSR---EGGHIEKYVDPLKEIAKKYRDDIL 287
              :|...|:.   |.::|..:|  |.:...:.:..|::   :.||...:.     :|.:
  Rat   258 DLIQGKDLLTAYYDVDYEKNTK--GSNYWRNRVMMVAKTFLDAGHKLNFA-----VASR------ 309

  Fly   288 FVTISSDEEDHTRIFEFFGMNKE--EVP--TIRLIKLEEDMAKYKPESDDLSAETIEAFLKKFLD 348
             .|.|.:..|       ||:...  |:|  .||..|.|:.:.:   |......:.:|.||:::.|
  Rat   310 -KTFSHELSD-------FGLESTTGEIPVVAIRTAKGEKFVMQ---EEFSRDGKALERFLQEYFD 363

  Fly   349 GKLKQHLLSQELPEDWDKNPVKVLVSSNFESVALDKSKSVLVEFYAPWCGHCKQLAPIYDQLAEK 413
            |.||::|.|:.:||. ::.||||:|:.:|:.:...:.|.||:|||||||||||.|.|.|.:|.||
  Rat   364 GNLKRYLKSEPIPET-NEGPVKVVVAESFDDIVNAEDKDVLIEFYAPWCGHCKNLEPKYKELGEK 427

  Fly   414 YKDNEDIVIAKMDSTANELES-IKISSFPTIKYFRKEDNKVI--DFNLDRTLDDFVKFLDANGEV 475
            ...:.:|||||||:|||::.| .::..|||| ||...:.|:.  .:...|.|:||:.:|...
  Rat   428 LSKDPNIVIAKMDATANDVPSPYEVKGFPTI-YFSPANKKLTPKKYEGGRELNDFISYLQRE--- 488

  Fly   476 ADSEPVEETEEEEEAPKK 493
            |.:.|:    .:||.|||
  Rat   489 ATNPPI----IQEEKPKK 502

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pdi	NP_001287070.1	ER_PDI_fam	27..474	CDD:273457	160/488 (33%)
		pdi_dom	33..133	CDD:273454	47/103 (46%)
		PDI_b_family	137..231	CDD:239279	27/118 (23%)
		PDI_b'_family	244..347	CDD:239280	21/109 (19%)
		PDI_a_PDI_a'_C	368..469	CDD:239293	48/103 (47%)
Pdia3	NP_059015.2	ER_PDI_fam	31..492	CDD:273457	161/492 (33%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	489..510		7/18 (39%)
		Prevents secretion from ER	507..510
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166350315
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0190
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53639
OrthoDB	1	1.010	-	-		D222522at33208
OrthoFinder	1	1.000	-	-		FOG0000292
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100478
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X241
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	9	8.660