DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pdi and P4hb

DIOPT Version :9

Sequence 1:	NP_001287070.1	Gene:	Pdi / 39651	FlyBaseID:	FBgn0286818	Length:	496	Species:	Drosophila melanogaster
Sequence 2:	NP_035162.1	Gene:	P4hb / 18453	MGIID:	97464	Length:	509	Species:	Mus musculus

Alignment Length:	507	Identity:	267/507 - (52%)
Similarity:	360/507 - (71%)	Gaps:	17/507 - (3%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 LICALFLAASYVAASAEAEVKVEEGVLVATVDNFKQLIADNEFVLVEFYAPWCGHCKALAPEYAK 68
            |:|.....|:.|.|.|..|   |:.|||....||::.:|.::::|||||||||||||||||||||
Mouse     6 LLCLALAWAARVGADALEE---EDNVLVLKKSNFEEALAAHKYLLVEFYAPWCGHCKALAPEYAK 67

  Fly    69 AAQQLAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSG---SPVEYSGGRQAADIIAWVT 130
            ||.:|..:.|.|:|||||||.|.:||:||.||||||:|||::|   ||.||:.||:|.||:.|:.
Mouse    68 AAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLK 132

  Fly   131 KKTGPPAKDLTSVADAEQFLKDNEIAIIGFFKDLESEEAKTFTKVANALDSFVFGVSSNADVIAK 195
            |:|||.|..|:..|.||..:..:|:.:||||||:||:.||.|...|.|:|...||::||:.|.:|
Mouse   133 KRTGPAATTLSDTAAAESLVDSSEVTVIGFFKDVESDSAKQFLLAAEAIDDIPFGITSNSGVFSK 197

  Fly   196 YEAKDNGVVLFKPFDDKKSVFEGELNEENLKKFAQVQSLPLIVDFNHESASKIFGGSIKSHLLFF 260
            |:...:||||||.||:.::.||||:.:|.|..|.:...|||:::|..::|.|||||.||:|:|.|
Mouse   198 YQLDKDGVVLFKKFDEGRNNFEGEITKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLF 262

  Fly   261 VSREGGHIEKYVDPLKEIAKKYRDDILFVTISSDEEDHTRIFEFFGMNKEEVPTIRLIKLEEDMA 325
            :.:.....:..:...|..|:.::..|||:.|.||..|:.||.||||:.|||.|.:|||.|||:|.
Mouse   263 LPKSVSDYDGKLSSFKRAAEGFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMT 327

  Fly   326 KYKPESDDLSAETIEAFLKKFLDGKLKQHLLSQELPEDWDKNPVKVLVSSNFESVALDKSKSVLV 390
            |||||||:|:||.|..|..:||:||:|.||:|||:||||||.||||||.:|||.||.|:.|:|.|
Mouse   328 KYKPESDELTAEKITEFCHRFLEGKIKPHLMSQEVPEDWDKQPVKVLVGANFEEVAFDEKKNVFV 392

  Fly   391 EFYAPWCGHCKQLAPIYDQLAEKYKDNEDIVIAKMDSTANELESIKISSFPTIKYF-RKEDNKVI 454
            ||||||||||||||||:|:|.|.|||:|:|:||||||||||:|::|:.||||:|:| ...|..||
Mouse   393 EFYAPWCGHCKQLAPIWDKLGETYKDHENIIIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVI 457

  Fly   455 DFNLDRTLDDFVKFLDANGE--VADSE--PVEET------EEEEEAPKKDEL 496
            |:|.:||||.|.|||::.|:  ..|.|  .:||.      |::::...||||
Mouse   458 DYNGERTLDGFKKFLESGGQDGAGDDEDLDLEEALEPDMEEDDDQKAVKDEL 509

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pdi	NP_001287070.1	ER_PDI_fam	27..474	CDD:273457	249/450 (55%)
		pdi_dom	33..133	CDD:273454	62/102 (61%)
		PDI_b_family	137..231	CDD:239279	42/93 (45%)
		PDI_b'_family	244..347	CDD:239280	49/102 (48%)
		PDI_a_PDI_a'_C	368..469	CDD:239293	68/101 (67%)
P4hb	NP_035162.1	ER_PDI_fam	26..476	CDD:273457	249/449 (55%)
		Thioredoxin_like	31..135	CDD:294274	62/103 (60%)
		PDI_b_family	139..234	CDD:239279	42/94 (45%)
		PDI_b'_family	246..349	CDD:239280	49/102 (48%)
		PDI_a_PDI_a'_C	370..472	CDD:239293	68/101 (67%)
		Prevents secretion from ER	506..509		2/2 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167846777
Domainoid	1	1.000	180	1.000	Domainoid score	I3501
eggNOG	1	0.900	-	-		E2759_KOG0190
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H55495
Inparanoid	1	1.050	535	1.000	Inparanoid score	I1183
Isobase	1	0.950	-	0	Normalized mean entropy	S1096
OMA	1	1.010	-	-		QHG53639
OrthoDB	1	1.010	-	-		D222522at33208
OrthoFinder	1	1.000	-	-		FOG0000292
OrthoInspector	1	1.000	-	-		oto92449
orthoMCL	1	0.900	-	-		OOG6_100478
Panther	1	1.100	-	-	LDO	PTHR18929
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2224
SonicParanoid	1	1.000	-	-		X241
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	17	16.750