Protein Alignment MICAL-like and Micall2

DIOPT Version :10

• Sequence 1: NP_648621.1
• Gene: MICAL-like / 39475
• FlyBaseID: FBgn0036333
• Length: 1010
• Species: Drosophila melanogaster
• Sequence 2: NP_001406493.1
• Gene: Micall2 / 288515
• RGDID: 1307875
• Length: 1006
• Species: Rattus norvegicus
• Alignment Length: 1145
• Identity: 286/1145 - (24%)
• Similarity: 424/1145 - (37%)
• Gaps: 335/1145 - (29%)

  Fly    14 KALEYWCRVVTQGYNGVKVENMTTSWRNGLAFCAIIHHFRPDLIDFDRLKADDIYENNDLAFTTA 78
            |||:.|||...:||..|.:.|||||:|:|||||||:|..|||||:|:.|:.::|||||.|||..|
  Rat     5 KALQEWCRQQCEGYRDVSITNMTTSFRDGLAFCAILHRHRPDLINFNALRKENIYENNKLAFQVA 69

  Fly    79 EKYLGIPALLDAADMVSYEVPDRLSILTYLSQFYKVL-GKS-------LKHP---KPEEPLGEES 132
            |:.|||||||||.|||:.::|||||||||:||:|... |:|       :|.|   ..||..|::.
  Rat    70 EEQLGIPALLDAEDMVALKIPDRLSILTYVSQYYNYFHGRSPIGGMAGMKRPSSDSTEELSGKKK 134

  Fly   133 EP-------------------------------------PQKVMHIVGMPRRDKCQKCNLPVFLA 160
            .|                                     |:.....||......|..|...|.|.
  Rat   135 VPSQPAKLSSPVPTQRLPLSPARTNPVVQRNEGVSERPSPKAAPGTVGSSVSSICGVCGKHVHLV 199

  Fly   161 ERVLVGKRAYHRTCLKCARCSSLLTPGSFYETEVNNIYCCETCPDEESEPESDILKLKTTTTDSP 225
            :|.|...|.|||:|.:|.:|||.|..|::..|....::   .|....||..|...||....:..|
  Rat   200 QRHLADGRLYHRSCFRCKQCSSTLHSGAYRATGEPGVF---VCTHHSSEAVSVSPKLSNLASRQP 261

  Fly   226 N----DKQMVAQSSDYSEAEDKQEDLEDNDIRTTDKPENFQPPSNKDEQNNELTI---------N 277
            .    |.:.:..|....|...:...|.   .||..........:.|.....|||.         :
  Rat   262 GGGIADTRPIGVSQKVLETNGEATPLR---ARTAAWEHAGGNRAAKGFVQTELTPPATSRVHVGS 323

  Fly   278 PVNPILSEERKISFIPLDEEDGGLIEQYNKSTTPVKPAIP---------------EKPKVS---- 323
            |..|.|    .:|.:.....:       :|:||.|..:.|               .:|.||
  Rat   324 PAGPRL----PMSTVTTTSAN-------SKATTHVTNSSPVGWSSSAQSSTGTSGSRPVVSPSAL 377

  Fly   324 ----TLPLDDEQHAGVEQNNDLAVSPENDIP--------------KEKL-----------KISSV 359
                ::|.......||:...:.:....:..|              :||.           ..||.
  Rat   378 GAHLSVPQGQAASKGVKTQLNSSTDSSSTAPTPAWTSSSSRTQQAREKFFHNLSPAPAPAPASSS 442

  Fly   360 SIYLEDDRLVVDAIHPDNLDKQEALNNTSDALIPESQEAPIPENNTQVAIKPEDHISPRKENKIF 424
            |.:......||.|  |..  |...|.|||.:.:|.:....:|.:.....:......:|.......
  Rat   443 SSHASRVPTVVTA--PSG--KVSPLVNTSTSKVPSATVVTVPTSKASTVVTAPTSKAPTVVTVPI 503

  Fly   425 SN-----TESCSKQEGVLPKQMD-----LESPKDK--------------VIETKASETDYPEDLN 465
            |.     |...||...|:.....     :.:|..|              |:.|.||:.....| |
  Rat   504 SKAPTVVTAPTSKVSTVVTVPTSKASTVVTAPTSKASTVVTVPTGRGHVVVNTSASKVSGVVD-N 567

  Fly   466 PFKDDDSSKGAN----------------PFDSSDDEVELLKAIPAQQSKGKVVPPRPPPPKIGLS 514
            |.::....:..:                |..||.....:|..:|..:     |||:.|..|:..|
  Rat   568 PAQESSREQALSVLRKALPGLTRAGSQAPSRSSPATSSVLITLPKNE-----VPPKVPSAKLSHS 627

  Fly   515 SISNPSEKPHSSPT--------------LSHGKKMPMPTPRI----SISKTQTPAK---PMTHQG 558
            :....|..|...||              |..|||.|..:|.|    ::|:.|...|   |.:.:|
  Rat   628 TTQAFSPTPKMEPTAPLSVGSTSWTSVSLQAGKKSPGISPGIGKTSAVSRPQAEVKGPGPTSQEG 692

  Fly   559 QKSSISSSSSEHLNSIRTFDRGADDRGSSISLPSANGPRKPLRASVGSPLRSEESSPTTSLSSIT 623
            |:        |.....|...:..|.|......|....||      .|...|...||..:|:....
  Rat   693 QE--------EGPEGWRARLKPVDKRALEQKEPVLAEPR------AGDTPRKASSSSDSSIHITL 743

  Fly   624 SPMRKKRQAPLPPIQTDFDSDPGFSKLSDEQKALLHTQLKAPNLGDSTRRLIPLDQSLLSDEATE 688
            :|:::||...|        :|.|.|             |.||:  ..:||
  Rat   744 TPIQQKRTPCL--------ADSGSS-------------LAAPS--PPSRR--------------- 770

  Fly   689 SSNYDESLSTSNADEEVNVVYRRILVPPT--------QPENTVERSKEDQKSPIVYNDFDRNVSP 745
                                 ::::||||        |||   .:.::||....
  Rat   771 ---------------------KKLVVPPTLDVSADWLQPE---LKKQDDQTRSC----------- 800

  Fly   746 LGHNKSTHGKWKRRK----------GPAPAVPIPPRKVLQRLPLQEIRHEFEIIAVQQLGLEKQG 800
                |.....|..|:          .|..||..|.|.....:..:|::.:.:.|..|...||.:|
  Rat   801 ----KEKTATWGTRESSAILDNDLVSPDEAVTSPVRLHPNYISQEELQRQLQDIERQLDALELRG 861

  Fly   801 VILEKMIRDRCERSLDATDTDGPESAEVLTNSKEVEDLILQLFELVNEKNELFRRQAELMYLRRQ 865
            |.|||.:|        |.:.|..|           :.|::..|.|::||..|.||::||||..:.
  Rat   862 VELEKRLR--------AAEGDASE-----------DGLMVDWFRLIHEKQLLLRRESELMYKSKD 907

  Fly   866 HRLEQEQADIEHEIRVLMGQPEHNKTDSDKAHEEVLINRLVKVVEMRNEVIDSLETDRVREARED 930
            ..||:.|.|::.|:|.||.:||..|:..|:..|:.|:|:.|..|..|::::|:|:.||:||..||
  Rat   908 QCLEERQLDLQGELRRLMEKPEGLKSPQDRKREQELLNQYVNTVNDRSDIVDNLDEDRLREQEED 972

  Fly   931 MSIKNRLHIYNSEREEPPAHPRSADKSSKKLSKKERKKLK 970
            ..:::.:.....:|:          ||...|||....|.|
  Rat   973 QMLESMIQNLGLQRK----------KSKSFLSKIWSSKSK 1002

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
MICAL-like	NP_648621.1	CH_MICALL2	11..115	CDD:409102	64/100 (64%)
		LIM_like_1	146..207	CDD:188784	19/60 (32%)
		PTZ00449	<411..727	CDD:185628	74/384 (19%)
		bMERB_dom	785..933	CDD:463467	51/147 (35%)
Micall2	NP_001406493.1	Forms an intramolecular interaction with the C-terminal coiled coil domain keeping the protein in a closed conformation. /evidence=ECO:0000250	1..261		99/258 (38%)
		CH_MICALL2	2..107	CDD:409102	64/101 (63%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	114..181		7/66 (11%)
		LIM_Mical_like_1	189..242	CDD:188828	19/55 (35%)
		Necessary and sufficient for interaction with actinins. /evidence=ECO:0000250	262..394		24/145 (17%)
		Chi1	311..>554	CDD:442692	42/257 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	311..450		23/149 (15%)
		Herpes_BLLF1	<457..>678	CDD:282904	45/228 (20%)
		Forms an intramolecular interaction with the N-terminal Calponin-homology and LIM zinc-binding domains-containing region keeping the protein in a closed conformation. /evidence=ECO:0000250	803..910		33/125 (26%)
		bMERB_dom	846..975	CDD:463467	51/147 (35%)
		Mediates interaction with RAB13 and is required for transition from the closed to the open conformation. /evidence=ECO:0000250	910..1006		34/103 (33%)