DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment MICAL-like and Micall2

DIOPT Version :9

Sequence 1:	NP_648621.1	Gene:	MICAL-like / 39475	FlyBaseID:	FBgn0036333	Length:	1010	Species:	Drosophila melanogaster
Sequence 2:	NP_777275.2	Gene:	Micall2 / 231830	MGIID:	2444818	Length:	1009	Species:	Mus musculus

Alignment Length:	1153	Identity:	271/1153 - (23%)
Similarity:	426/1153 - (36%)	Gaps:	348/1153 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly    14 KALEYWCRVVTQGYNGVKVENMTTSWRNGLAFCAIIHHFRPDLIDFDRLKADDIYENNDLAFTTA 78
            |||:.|||...:||..|.:.|||||:|:|||||||:|..|||||:|..|:.::|||||.|||..|
Mouse     5 KALQEWCRQQCEGYRDVSITNMTTSFRDGLAFCAILHRHRPDLINFSALRKENIYENNKLAFQVA 69

  Fly    79 EKYLGIPALLDAADMVSYEVPDRLSILTYLSQFY-----------------------------KV 114
            |:.|||||||||.|||:.:||||||||||:||:|                             |.
Mouse    70 EEQLGIPALLDAEDMVALKVPDRLSILTYVSQYYNYFHGRSPIGGMAGIKRPSSDSTEELSGKKG 134

  Fly   115 LGKSLKHPKPEE----PL--------------GEESEPPQKVMHIVGMPRRDKCQKCNLPVFLAE 161
            |.:..|.|.|.:    ||              |.:...|:......|......|..|...|.|.:
Mouse   135 LSQPAKLPSPAQTQRSPLSPARTNPVVQRNEGGSQRPSPKAAPGTAGSSVSSICGVCGKHVHLVQ 199

  Fly   162 RVLVGKRAYHRTCLKCARCSSLLTPGSFYETEVNNIYCCETCPDEESEPESDILKLKTTTTDSPN 226
            |.|...|.|||:|.:|.:|||.|..|::..|....::   .|....||          .|:.||.
Mouse   200 RHLADGRLYHRSCFRCKQCSSTLHSGAYRATGEPGVF---VCTHHSSE----------VTSVSPK 251

  Fly   227 DKQMVAQSSDYSEAEDKQEDLEDNDIRTTDKPENFQPPSNKDEQNNELTINPVNPILSEERKISF 291
            ...:.::              :...:....:|  |.......|.|.|.|
Mouse   252 SSNLASR--------------KPGGVTADTRP--FGVSWTVQEANGEGT---------------- 284

  Fly   292 IPLD------EEDGGLIEQYNKSTTPVKPAIPEKPKVSTLPLDDEQHAGVEQNNDLAVSPENDIP 350
             ||.      |..||.........|.:||           |...:.|.|......|..
Mouse   285 -PLRVRTAAWEHAGGNTTAKGFVQTELKP-----------PSTSQVHVGSSAGPKLPT------- 330

  Fly   351 KEKLKISSVSIYLEDDRLVVDAIHPDNLDKQEALNNTSDALIPESQEAPI----PENNTQVAIKP 411
               :.:::.|:                         ||.||...:..:||    |..::......
Mouse   331 ---ITVTTTSV-------------------------TSKALTHVTNSSPIGWSSPAQSSPANFNS 367

  Fly   412 EDHISPRKENKIFSNTESCSKQEGVLPKQMDLESPKDKVIET-----KASETDYPEDLNPFKDDD 471
            ...:||...|.....::..:..:|| ..|::|.|.......|     .||:|....: ..|:...
Mouse   368 RPVVSPSARNTHLPGSQGQTASKGV-KTQLNLNSESSNTAVTPAWTSSASKTQQARE-KFFQTPP 430

  Fly   472 S--SKGANPFDSSDDEVELLKAIPAQQSKGKVVPPRPPPPKIGL-------SSISNPSEKPH--- 524
            |  :..:.|..:...:|..:..:|..:....|..|....|.:..       :.:|.|:.|..
Mouse   431 SAPAPASAPAPAPTSKVPTVVTVPTSKVPNVVTAPTSKVPTVVTVPTSKVPTVVSAPTSKVPTVV 495

  Fly   525 SSPTLSHGKKMP---------------MPTPR----ISISKTQTPAKPMTHQGQKSSISSSSSEH 570
            |:||    .|:|               .||.:    :|.:..:.|.....|.|:..::.::|:..
Mouse   496 SAPT----SKVPTVVNSTNSRVTTVVNAPTSKVPTVVSATNGRVPTVVTAHTGRVPAVMNTSASK 556

  Fly   571 LNSIRTFDRGADDRGSSISLPSANGPRKPLRASVGSPLRS-EESSPTTSLSSITSPMRK-KRQAP 633
            ::.:.........|..::|:     .||.|.|..||..:: ..|.|.||...:|.|..: .::.|
Mouse   557 VSPVVDAPAQESSREQALSV-----LRKALPALTGSGTQAPNRSFPATSSVLVTLPKNEVPQKVP 616

  Fly   634 LPPIQTDFDSDPGFS-KLS-------DEQKALLHTQLKAPNLGDST------------------- 671
            ...:.......|.|: ||.       ......|....|:|::....
Mouse   617 SDKLSALTTQTPNFTIKLEPSAPVNVGNTAVFLQAGKKSPSISPRVGKTSVGSRPQAEVAGVKGP 681

  Fly   672 ----------------RRLIPLDQSLLSDEATESSN--------YDES-LSTSNADEEVNVVY-- 709
                            .||.|:|:...:..:.|...        .|.| .::|::|..|::..
Mouse   682 GPISQEGQEEGPEGWRARLKPVDKKTPAGRSLEQKEPVLAEPRIGDTSRKASSSSDSSVHITLTS 746

  Fly   710 --------------------------RRILVPPT--------QPENTVE----RSKEDQKSPIVY 736
                                      :::.|||:        |||...:    ||.:::|||.
Mouse   747 IQHKRKPCPAGSGPSPAALSPSPSHRKKLAVPPSLDVSADWLQPEPKKQEDGTRSCKEEKSPT-- 809

  Fly   737 NDFDRNVSPLGHNKSTHGKWKRRKG---------PAPAVPIPPRKVLQRLPLQEIRHEFEIIAVQ 792
                              :|.|.:.         |..||..|.|.....:|.:|::.:.:.|..|
Mouse   810 ------------------RWSRERSAVLDSGLAPPGEAVTSPVRLHPDYIPQEELQRQLQDIESQ 856

  Fly   793 QLGLEKQGVILEKMIRDRCERSLDATDTDGPESAEVLTNSKEVEDLILQLFELVNEKNELFRRQA 857
            ...||.:||.|||.:|        |.:.|..|           :.|::..|.|::||..|.|.::
Mouse   857 LDALELRGVELEKRLR--------AAEGDASE-----------DSLMVDWFRLIHEKQLLLRLES 902

  Fly   858 ELMYLRRQHRLEQEQADIEHEIRVLMGQPEHNKTDSDKAHEEVLINRLVKVVEMRNEVIDSLETD 922
            ||||..:..|||::|.|::.|:|.||.:||..|:..|:..|:.|:::.|..|..|::::|.|:.|
Mouse   903 ELMYKSKDQRLEEQQLDLQGELRRLMDKPEGLKSPQDRQREQELLSQYVNTVNDRSDIVDFLDED 967

  Fly   923 RVREAREDMSIKNRLHIYNSEREEPPAHPRSADKSSKKLSKKERKKLK 970
            |:||..||..::|.:.....:|:          ||...|||....|.|
Mouse   968 RLREQEEDQMLENMIQNLGLQRK----------KSKSFLSKIWSSKSK 1005

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
MICAL-like	NP_648621.1	CH	14..111	CDD:278723	63/96 (66%)
		LIM_like_1	146..207	CDD:188784	19/60 (32%)
		DUF3585	778..933	CDD:288945	52/154 (34%)
Micall2	NP_777275.2	Forms an intramolecular interaction with the C-terminal coiled coil domain keeping the protein in a closed conformation	1..260		99/281 (35%)
		CH	5..102	CDD:278723	63/96 (66%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	114..180		9/65 (14%)
		LIM_Mical_like_1	188..241	CDD:188828	19/55 (35%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	247..268		2/34 (6%)
		Mediates targeting to the cell plasma membrane	261..805		101/619 (16%)
		Necessary and sufficient for interaction with actinins	261..393		31/197 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	348..447		18/100 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	655..834		27/198 (14%)
		Forms an intramolecular interaction with the N-terminal Calponin-homology and LIM zinc-binding domains-containing region keeping the protein in a closed conformation	806..913		36/145 (25%)
		DUF3585	842..978	CDD:288945	52/154 (34%)
		Mediates interaction with RAB13 and is required for transition from the closed to the open conformation	913..1009		34/103 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	139	1.000	Domainoid score	I4772
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002858
OrthoInspector	1	1.000	-	-		otm42521
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R5658
SonicParanoid	1	1.000	-	-		X4926
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	9	8.900