DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sti and MAD1L1

DIOPT Version :9

Sequence 1:	NP_001261768.1	Gene:	sti / 39429	FlyBaseID:	FBgn0002466	Length:	1858	Species:	Drosophila melanogaster
Sequence 2:	NP_001013858.1	Gene:	MAD1L1 / 8379	HGNCID:	6762	Length:	718	Species:	Homo sapiens

Alignment Length:	775	Identity:	179/775 - (23%)
Similarity:	311/775 - (40%)	Gaps:	173/775 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly   521 QIIKEKTMELTTCKTQIKTLQSSAKIDEEMW----SKKEATITDLLRLNRQKYEEAKIASEQRYE 581
            |:..:::|:|.....||::.....:::.|..    |.|.|         |.:.|.|...|.:.||
Human    42 QMQYQQSMQLEERAEQIRSKSHLIQVEREKMQMELSHKRA---------RVELERAASTSARNYE 97

  Fly   582 KQLADKKQELASTLQKLDARELEFNAKFEECKHLSMKLQNYKDMLQQI--KEQNLKSETNHEEQR 644
            ::: |:.|||.:.:::|..||.....|.:|      :|:..:...|.:  ..:.|:.:.:...|.
Human    98 REV-DRNQELLTRIRQLQEREAGAEEKMQE------QLERNRQCQQNLDAASKRLREKEDSLAQA 155

  Fly   645 RQMAELYEQKLTDLRKKVRDSQDTNRRMTMEIKEIRTELDESISSSKSTQEAKNATERNIEEILR 709
            .:.....:.::::|:..|.|.:...:|:..|.:|::.:||   ...|..|||    .:.|:|:
Human   156 GETINALKGRISELQWSVMDQEMRVKRLESEKQELQEQLD---LQHKKCQEA----NQKIQEL-- 211

  Fly   710 RLNEEIASNNELHAEKVK-LETKLQLKENETQEVR------AECHRLEREL-QLAECRCQLAESS 766
            :.::|..::   |.:::| ||.||.|:|.:...|:      ....|||||| ||.|....|.|..
Human   212 QASQEARAD---HEQQIKDLEQKLSLQEQDAAIVKNMKSELVRLPRLERELKQLREESAHLREMR 273

  Fly   767 LATQVSPYETAPGSLTELNAIEDQL-----RADLLAAKESENHQK-------GRADQLQTLVTKL 819
                    ||......||..::.:|     ..:.|...|.||.:.       .|.||...|..:.
Human   274 --------ETNGLLQEELEGLQRKLGRQEKMQETLVGLELENERLLAKLQSWERLDQTMGLSIRT 330

  Fly   820 EQMLERF------NEQSLSPTKSHSSRKQEGETVGDMLERQNEKLEDKLAAVREQMIVERQAART 878
            .:.|.||      .|.:|....|..:....|      ||:..::|:::|..|..|::.||:...|
Human   331 PEDLSRFVVELQQRELALKDKNSAVTSSARG------LEKARQQLQEELRQVSGQLLEERKKRET 389

  Fly   879 ANLSLWKVEKQLEEALSEK--------------------KLLARRMELTEDRIKKVQNASDEAQR 923
            ......:::|::.....|:                    ..|.|||...||.::||.:.|.|.:.
Human   390 HEALARRLQKRVLLLTKERDGMRAILGSYDSELTPAEYSPQLTRRMREAEDMVQKVHSHSAEMEA 454

  Fly   924 MLKTSQEETRQRESRIEELKQELAAAKRDVLKEHRQWEKAEQERMKCKSEIIEHLANVHRLEQQE 988
            .|..:.||...::.|.:.|:.||...|       .|...|||..:..:.|.......|..||.:.
Human   455 QLSQALEELGGQKQRADMLEMELKMLK-------SQSSSAEQSFLFSREEADTLRLKVEELEGER 512

  Fly   989 TELRQKLRQIQSRFDGVTLEQKNTIRELQEEREKSRKANDSCLVLQKELKQLTDNFQRLKYACSI 1053
            :.|.::.|.::::     ||:    |.||.:.::||..     ||...|.           ..|:
Human   513 SRLEEEKRMLEAQ-----LER----RALQGDYDQSRTK-----VLHMSLN-----------PTSV 552

  Fly  1054 TDSQLTEVETMLKSEQERNKSQKSQLDTLHEKLR--ERNDQL-TDLRKQLTTVESEKRLAEQRAQ 1115
            ...:|.|..:.|::|.||          |...||  ||...: .||.....::.|.|.:||.:.|
Human   553 ARQRLREDHSQLQAECER----------LRGLLRAMERGGTVPADLEAAAASLPSSKEVAELKKQ 607

  Fly  1116 VLASEIDELRLNLKEQQKKLVAQQDQLVEQTNALFA--------TQERAELLDGQNANYEAQ--- 1169
            |.::|       ||.|:.|.|. |.::.|...|.:.        |.|....|....|.:...
Human   608 VESAE-------LKNQRLKEVF-QTKIQEFRKACYTLTGYQIDITTENQYRLTSLYAEHPGDCLI 664

  Fly  1170 ---TADSNREMVSLKEENARILSELFHKKEEVGNLQAEIRGLESAQA---NLHAEIDSLQ 1223
               |:.|..:|..|:.|.:..:.||         ::..:|..:|..|   :|..|:.|.|
Human   665 FKATSPSGSKMQLLETEFSHTVGEL---------IEVHLRRQDSIPAFLSSLTLELFSRQ 715

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sti	NP_001261768.1	PKc_like	111..452	CDD:304357
		S_TKc	113..383	CDD:214567
		Smc	487..1222	CDD:224117	177/772 (23%)
		CNH	1503..1774	CDD:279162
MAD1L1	NP_001013858.1	MAD	54..716	CDD:283261	176/763 (23%)
		Nuclear localization signal. /evidence=ECO:0000269\|PubMed:19010891	79..82		2/11 (18%)
		Important for interaction with IK. /evidence=ECO:0000269\|PubMed:22351768	301..340		10/38 (26%)
		Necessary for interaction with NEK2. /evidence=ECO:0000269\|PubMed:14978040	380..532		36/167 (22%)
		Important for interaction with IK. /evidence=ECO:0000269\|PubMed:22351768	439..480		13/40 (33%)
		Necessary for interaction with MAD2L1. /evidence=ECO:0000269\|PubMed:19010891	540..551		3/26 (12%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	0	0.000