DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sti and ROCK1

DIOPT Version :9

Sequence 1:	NP_001261768.1	Gene:	sti / 39429	FlyBaseID:	FBgn0002466	Length:	1858	Species:	Drosophila melanogaster
Sequence 2:	NP_005397.1	Gene:	ROCK1 / 6093	HGNCID:	10251	Length:	1354	Species:	Homo sapiens

Alignment Length:	1671	Identity:	395/1671 - (23%)
Similarity:	678/1671 - (40%)	Gaps:	499/1671 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly    66 LLDAFCLLYNECDKDTLKKRDRNIAEFVNKFRPIIEETRKLRVNADDFLIKTLIGQGYFGNVHLV 130
            |||....|..:.|...|:| ::||..|:::::..|.:.|.||:.|:|:.:..:||:|.||.|.||
Human    30 LLDGLDALVYDLDFPALRK-NKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLV 93

  Fly   131 VERQTNDIYAMKKIKK-SVVTTSQVK---EERDIMSIRNSEWLINLQYAFQDNDNLYLVMEYMPG 191
            ..:.|..:||||.:.| .::..|...   ||||||:..||.|::.|.|||||:..||:|||||||
Human    94 RHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPG 158

  Fly   192 GDLLSLMSRHGPFDEDLARFYLAELTVALHTLHEMGYVHRDIKPENILIDRFGHIKLADFGNAAA 256
            |||::|||.: ...|..||||.||:.:||..:|.||::|||:||:|:|:|:.||:||||||....
Human   159 GDLVNLMSNY-DVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMK 222

  Fly   257 LDRDGHVLSLSPVGTPDYIAPELLQTISTYKLSKSMHDVSRIVSCDYWSMGIIGYELICETTPFH 321
            ::::|.|...:.|||||||:||:|::        ...|......||:||:|:..||::...|||:
Human   223 MNKEGMVRCDTAVGTPDYISPEVLKS--------QGGDGYYGRECDWWSVGVFLYEMLVGDTPFY 279

  Fly   322 EDNVHETYSKILSHCEESHLKELISFPADLKVSVNYRNLIESLVTNPSKRL---SYERIKNHPFF 383
            .|::..|||||::|      |..::||.|..:|...:|||.:.:|:...||   ..|.||.|.||
Human   280 ADSLVGTYSKIMNH------KNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFF 338

  Fly   384 --SEIPWGSIRSQVPPIIPTVRSDDDTSNFEDGIRHKTRREQGVAKKSLTTNMKSNDFSGKDLPF 446
              .:..|.::|..|.|::|.:.||.|||||:|....|...|.....|:         |.|..|||
Human   339 KNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKA---------FVGNQLPF 394

  Fly   447 IGYSF---------VHMEKSAISATTDEKLQEKLKELLQKLKTRENEISMLKQDLLRAQQSLRKT 502
            :|:::         .:...:..|:..|:.|||.|::.:.||:.:.:....||.::   :|..| |
Human   395 VGFTYYSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEM---EQKCR-T 455

  Fly   503 DNKSQVVADAKMEIKKLQQIIKEKTMELTTCKTQIKTLQSSAKIDEEMWSKK--EATITDLLRLN 565
            .|            .||.:|:||                    :|||...::  |:|::.:
Human   456 SN------------IKLDKIMKE--------------------LDEEGNQRRNLESTVSQI---- 484

  Fly   566 RQKYEEAKIASEQRYEKQLADKKQELASTLQKLDARELEFNAKFEECKHLSMKLQNYKDMLQQIK 630
                |:.|:..:.|..:.....:||                  .|:.:::..::...||.|:.:|
Human   485 ----EKEKMLLQHRINEYQRKAEQE------------------NEKRRNVENEVSTLKDQLEDLK 527

  Fly   631 EQNLKSETNHEEQRRQMAELYEQKLTDLRKKVRDSQDTNRRMTMEIKEIRTELDESISSSKS-TQ 694
            :.:            |.::|..:||:.|:|::.::.|.          :|||.|.::...|| |:
Human   528 KVS------------QNSQLANEKLSQLQKQLEEANDL----------LRTESDTAVRLRKSHTE 570

  Fly   695 EAKNATERNIEEILRRLNEEIASNNELHAEKVKLETKLQLKENETQEVRAECHRLERELQLAECR 759
            .:|:.::      |..||.|:...|.:
Human   571 MSKSISQ------LESLNRELQERNRI-------------------------------------- 591

  Fly   760 CQLAESSLATQVSPYETAPGSLTELNAIEDQLRADLLAAKESENHQKGRADQLQTLVTKLEQMLE 824
              |..|...|....|               ||:|.|.|.:....|.......||..:|.|::.::
Human   592 --LENSKSQTDKDYY---------------QLQAILEAERRDRGHDSEMIGDLQARITSLQEEVK 639

  Fly   825 RFNEQSLSPTKSHSSRKQEGE--TVGDML---ERQNEKLEDKLAAVREQMIVERQAARTANLSLW 884
            ..         .|:..|.|||  ...|||   |::...||..|                 |..|.
Human   640 HL---------KHNLEKVEGERKEAQDMLNHSEKEKNNLEIDL-----------------NYKLK 678

  Fly   885 KVEKQLEEALSEKKLLARRMELTEDRIKKVQNASD----EAQRMLKTSQEETRQRESRIEELKQE 945
            .::::||:.::|.|:...|:   .|:.:.::.|..    |.::.||..:|...:.|:|:.:::::
Human   679 SLQQRLEQEVNEHKVTKARL---TDKHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVQIEKQ 740

  Fly   946 LAAAKRDVLKEHRQWEKAEQERMKCKSEIIEHL-ANVHRLEQQETELRQKLRQIQSR-------- 1001
            .:....|:        |..|::       :||| .|..|:|.:...|..:|.|..::
Human   741 CSMLDVDL--------KQSQQK-------LEHLTGNKERMEDEVKNLTLQLEQESNKRLLLQNEL 790

  Fly  1002 ---------FDGVTLEQKNTIRELQEEREKSRKANDSCLVLQKELKQLTDNFQRLKYACSITDSQ 1057
                     ..|:..:.|..|..|.|.:.          :|:.||.|||..::.       .:.|
Human   791 KTQAFEADNLKGLEKQMKQEINTLLEAKR----------LLEFELAQLTKQYRG-------NEGQ 838

  Fly  1058 LTEVETMLKSEQERNKSQKSQLDTLHEKLRERN----DQLTDLRKQLTTVESEKRLAEQRAQVLA 1118
            :.|::..|::||..:...|:|:..|.|::.|:|    .::.:|:.:..|:.::..|||.:|
Human   839 MRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKA---- 899

  Fly  1119 SEIDELRLNLKEQQKKLVAQQDQLVEQTNALFATQERAELLDGQNANYEAQTADS--NREMVSLK 1181
             |.::|...|.|:|...:.|:.:..       |::.|.|:.|..:.....:.|:|  .:::..|:
Human   900 -ESEQLARGLLEEQYFELTQESKKA-------ASRNRQEITDKDHTVSRLEEANSMLTKDIEILR 956

  Fly  1182 EENARILSEL--------FHKKEEVGNLQAEI-------RGLESAQANLHAEIDSLQD------- 1224
            .||..:..::        ..|:||:.||:|..       |.|::...|..|||.:.:|
Human   957 RENEELTEKMKKAEEEYKLEKEEEISNLKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKK 1021

  Fly  1225 ------TLAEKEQFYVQRDI-----KSNATLAQHKKLIDYLQLK--------------------- 1257
                  ...|||...:|.::     |.|..:.:|:|.::.:|.:
Human  1022 ANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECAHRNELQMQLASKESD 1086

  Fly  1258 VEDLSAKKKKTLADKLFGSSHTNKENVSPNDVESSI---LYRALKEELKREQKMNSLLKEQLAQL 1319
            :|.|.| |...|:|....:|..:.:....|..||.|   |....:..:||..     .|:|...:
Human  1087 IEQLRA-KLLDLSDSTSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYG-----WKKQYVVV 1145

  Fly  1320 NGTATLRSPRKSAAVNGDSDAPKQRPVSIAALPR----SPQKQQQPLKRTTSQ------------ 1368
                   |.:|....|.:.|..:..|..:..:.:    .|..|....:..|.:
Human  1146 -------SSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANE 1203

  Fly  1369 ------VELKTTAEKPTKVTIENQAHHRFELALQESKVDAVNCVVCEKAVVAGSPFW-------- 1419
                  ||:: ..::..|...:|...|.|...|...   ..||..|.|      |.|
Human  1204 GECRKDVEME-PVQQAEKTNFQNHKGHEFIPTLYHF---PANCDACAK------PLWHVFKPPPA 1258

  Fly  1420 -KCKECKDVTHRKCRSNVQSHCGSTKPTAPSADDLSSIQSVSSLTLDSVDVAGGTTSGGEYIGSL 1483
             :|:.|    |.||.
Human  1259 LECRRC----HVKCH-------------------------------------------------- 1269

  Fly  1484 VYSSDGAEDQARKEIEVNCAFEV-----AEQQILLLGCNTGLYAYHLDSQR--LVHISGLESVSC 1541
                   .|...|:.::.|..:|     :.:.:|||.|:.       |.|:  :.|
Human  1270 -------RDHLDKKEDLICPCKVSYDVTSARDMLLLACSQ-------DEQKKWVTH--------- 1311

  Fly  1542 MSICKRLAKAIMVGTVGEKLYQCDYRQLESRCQSSSSCHKPVLETS 1587
              :.|::.|....|.|     :...|.|.:|..::.|..|.|..||
Human  1312 --LVKKIPKNPPSGFV-----RASPRTLSTRSTANQSFRKVVKNTS 1350

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sti	NP_001261768.1	PKc_like	111..452	CDD:304357	143/358 (40%)
		S_TKc	113..383	CDD:214567	118/276 (43%)
		Smc	487..1222	CDD:224117	154/785 (20%)
		CNH	1503..1774	CDD:279162	20/92 (22%)
ROCK1	NP_005397.1	STKc_ROCK1	2..406	CDD:270772	158/400 (40%)
		S_TKc	76..338	CDD:214567	118/276 (43%)
		Interaction with FHOD1. /evidence=ECO:0000269\|PubMed:18694941	368..727		104/541 (19%)
		SbcC	<422..1027	CDD:223496	161/822 (20%)
		HR1_ROCK1	489..554	CDD:212029	15/104 (14%)
		SHROOM3 binding. /evidence=ECO:0000269\|PubMed:22493320	707..946		58/282 (21%)
		Rho_Binding	949..1014	CDD:286056	15/64 (23%)
		RHOA binding	998..1010		4/11 (36%)
		Auto-inhibitory	1115..1354		58/342 (17%)
		PH_ROCK	1119..1225	CDD:269948	18/118 (15%)
		C1	1229..1275	CDD:237996	15/115 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1320..1354		10/36 (28%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0612
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D759391at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.820