Protein Alignment Ncc69 and Slc12a4

DIOPT Version :10

• Sequence 1: NP_001261758.1
• Gene: Ncc69 / 39410
• FlyBaseID: FBgn0036279
• Length: 1207
• Species: Drosophila melanogaster
• Sequence 2: NP_001240733.1
• Gene: Slc12a4 / 20498
• MGIID: 1309465
• Length: 1087
• Species: Mus musculus
• Alignment Length: 1296
• Identity: 322/1296 - (24%)
• Similarity: 505/1296 - (38%)
• Gaps: 409/1296 - (31%)

  Fly   103 DKPSR--FKDLQTTTRFQVDPQNEESDESNDSQ-EERE------LLD----NEYDTKYGKSFRHF 154
            |.|.|  :.:|:..:......:.|..|...:.| ..||      .||    |:|   |.::...|
Mouse    10 DGPRRGDYDNLEGLSWVDYGERAEREDSDGEGQGNHRENSPFLCPLDASRGNDY---YDRNLALF 71

  Fly   155 TREALPRLDNYRNMMSIQAAYRPTLDELHNATLVGK------NTHSLTRNQDPESGILNG----- 208
            ..|    ||           .||.:     ::|:||      .|.....:::.|||  .|
Mouse    72 EEE----LD-----------IRPKV-----SSLLGKLVSYTNLTQGAKEHEEAESG--EGGRRRA 114

  Fly   209 --VLKFGWIKGVLVRCLLNIWGVMLFLRLSWVVGQAGVIEGFVLILTTTAVTTITALSMSAISTN 271
              ....|.:.||.:.||.||:||:|||||:|:||.|||::..:::|.....|.:||:|||||:||
Mouse   115 AKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATN 179

  Fly   272 GVIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACAMYVVGFCESMLAMMTTFGWEIIDGGVQD 336
            ||:..||:|:||||||||||||::||.|.|....|.|||::|..|.:|..:..........|..|
Mouse   180 GVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHD 244

  Fly   337 --------VRIIGCITILLLLIIVVVGMEWEAKAQIGLLIILLVAIGDFVIGS------------ 381
                    :|:.|.|.:.|:.::|.||:::..|.....|..::::|.....|.
Mouse   245 MSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPV 309

  Fly   382 -FIG----------------------------------PKSDAEMA------------KGFLGYN 399
             .:|                                  |...|:..            .|..|..
Mouse   310 CMLGNRTLSRDQFDICAKTVVVDNETVATRLWTFFCHSPNLTADSCDPYFLLNNVTEIPGIPGAA 374

  Fly   400 ATLFKNNLFADYRPEKGGI--QH------------------------DFFSVFAIFFPAATGILA 438
            |.:.:.||::.|. |||.:  :|                        .|..:..||||:.|||:|
Mouse   375 AGVLQENLWSAYL-EKGEVVEKHGLPSTDTLGLKESLSLYVVADIATSFTVLVGIFFPSVTGIMA 438

  Fly   439 GANISGDLKDPQKSIPKGTILAIVITTGTYLIMVLQCGA----TVARDATGN--LSDVVNGTFAF 497
            |:|.||||:|.|||||.|||||||.|:..|...|:..||    .|.||..|:  ..::|.||.|:
Mouse   439 GSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGVSRNLVVGTLAW 503

  Fly   498 LDCQPGECNYGLQNSFQVIELVSAFGPLIYAGCYAATLSSALASLVSAPKVFQALCKDELYPKIV 562
                |...                   :|..|.:.:|..:.|.||..||::.||:.||.:.|.:.
Mouse   504 ----PSPW-------------------VIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLR 545

  Fly   563 WFAKGYGK-NNEPVRGYVLTFIIACAFILIGELNLIAPLISNFFLAAYMLINFSTFHASLAKPVG 626
            .|  |:|| |.||....:||.:||...|||..|:::||::|.|||..|:.:|.:....:|.:...
Mouse   546 VF--GHGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPN 608

  Fly   627 WRPTFKYYNMWLSLLGAILCVAVMFLISWATALITFAAVLALYLIVAYRKPDVNWGSTTQAQTYK 691
            |||.||||:..||.||..||:|:||:.||..||:.......:|..:.|:..:..||...:..:..
Mouse   609 WRPRFKYYHWTLSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLS 673

  Fly   692 NALMSVQQLNNVEEHVKNYRPQILVLSGLPN----TRPVLVDLAYMLTKNLSLLVCGHVLKGSSS 752
            .|..::.:|.....|.||:|||:|||..|..    ..|.|:..|..|.....|.:.|.|::||..
Mouse   674 AARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFL 738

  Fly   753 QKY--RTYLQERAGNWFRKHRVKGFYALVDGEDFESGTRALMQATGIGKLKPNIILMGYKTDWQT 815
            :.|  ....::...|.....:||||..:|.......|...|:|:.|:|.::.|.:::|:...|:.
Mouse   739 ESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQ 803

  Fly   816 CDHKELDQYFNVMHKALDMYLSVAILRVPQGLDCSQVLGSQD--GWKTVSDVPRTLQPNESSGDL 878
                                                   |:|  .|||..|..|
Mouse   804 ---------------------------------------SEDPRAWKTFIDTVR----------- 818

  Fly   879 QAVDSSVRNGLSGSIDSLSRNVSQEDRNRNQLVHSEQNSLKIVKLRFKQGLRRMRSWPGAASSTS 943
                                           ...:...:|.:.|
Mouse   819 -------------------------------CTTAAHLALLVPK--------------------- 831

  Fly   944 DLSFIAGNQSKDVSGMPDPLDAKSANLVSNSLRKSKLKHDDPASLYKGPGGAELPKEVLADLTQF 1008
            :::|...|..:.:.|
Mouse   832 NIAFYPSNHERYLDG-------------------------------------------------- 846

  Fly  1009 TRKRSHAVIDVWWLYDDGGLTLLLPYIISTRRTWQSCKLRVYALANKNSELEFEQRSMASLLSKF 1073
                 |  |||||:..|||:.:|||:::...:.|:.|::|::.:|..:......::.:|..|...
Mouse   847 -----H--IDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHL 904

  Fly  1074 RID---------YSDLTLIPDITKKPQETSTQFFNELIKDFVVTEKDGENGTSSRATLNED---- 1125
            |::         .||::..........|..:|...::  ....||:|.|      |.|.:|
Mouse   905 RLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQM--RLTKTERDRE------AQLVKDRHSA 961

  Fly  1126 ---DALITD--DDLLAVQDK------TNRYL---------------------------------R 1146
               ::|.:|  ::.:|..||      .::|:                                 :
Mouse   962 LRLESLYSDEEEESVAGADKIQMTWTRDKYMAEPWDPSHAPDNFRELVHIKPDQSNVRRMHTAVK 1026

  Fly  1147 LREYLREQSTKSDLVVMTLPMPRKNIVSAPLYMAWLESLSRDMPPFLFVRGNQTSVLTFYS 1207
            |.|.:..:|..:.||::.:|.|.||......||.:||.|:..:...|.|||....|:|.||
Mouse  1027 LNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS 1087

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ncc69	NP_001261758.1	AA_permease_2	155..1207	CDD:459263	305/1229 (25%)
Slc12a4	NP_001240733.1	2a30	44..1087	CDD:273347	313/1260 (25%)
		Scissor helix. /evidence=ECO:0000250|UniProtKB:Q9UP95	667..683		1/15 (7%)