DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Klp68D and kif3b

DIOPT Version :9

Sequence 1:	NP_001261726.1	Gene:	Klp68D / 39332	FlyBaseID:	FBgn0004381	Length:	784	Species:	Drosophila melanogaster
Sequence 2:	NP_001093615.1	Gene:	kif3b / 100101641	ZFINID:	ZDB-GENE-050119-3	Length:	775	Species:	Danio rerio

Alignment Length:	832	Identity:	366/832 - (43%)
Similarity:	506/832 - (60%)	Gaps:	117/832 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 SSQTPNECVQVVVRCRPMSNRERSERSPEVVNVYPNRGVVELQNVVDGNK-EQRKVFTYDAAYDA 75
            |....:|.|:||||||||:.:||......||:|....|.|.:.|....:. |..||||:|:.||.
Zfish     2 SKSKSSESVKVVVRCRPMNEKERVANFNRVVSVDVKLGQVAVCNPRGASSHEHPKVFTFDSVYDW 66

  Fly    76 SATQTTLYHEVVFPLVSSVLEGFNGCIFAYGQTGTGKTFTMEGVRGNDELMGIIPRTFEQIWLHI 140
            ::.|..||.|...|||.|||.||||.||||||||||||:||||||.:.|..|:||.:||.|:.||
Zfish    67 NSKQMELYDETFRPLVDSVLFGFNGTIFAYGQTGTGKTYTMEGVRNDPERRGVIPNSFEHIFTHI 131

  Fly   141 NRTENFQFLVDVSYLEIYMEELRDLL-KPNSKHLEVRER-GSGVYVPNLHAINCKSVEDMIKVMQ 203
            :.::|.|:||..||||||.||:|||| |..::.||::|| .:||||.:|.:...|||.::..||.
Zfish   132 SCSQNQQYLVRASYLEIYQEEIRDLLSKDQARRLELKERPDTGVYVKDLSSFVTKSVREIEHVMN 196

  Fly   204 VGNKNRTVGFTNMNEHSSRSHAIFMIKIEMC-----DTETNTIKVGKLNLIDLAGSERQSKTGAS 263
            |||:||:||.||||||||||||||:|.|| |     |.| |.|:||||||:||||||||:||||.
Zfish   197 VGNQNRSVGATNMNEHSSRSHAIFVITIE-CSELGPDGE-NHIRVGKLNLVDLAGSERQTKTGAQ 259

  Fly   264 AERLKEASKINLALSSLGNVISALAES-SPHVPYRDSKLTRLLQDSLGGNSKTIMIANIGPSNYN 327
            .||||||:||||:||:||||||||.:. |.|:||||||||||||||||||::|:|:|||||::||
Zfish   260 GERLKEATKINLSLSALGNVISALVDGRSTHIPYRDSKLTRLLQDSLGGNARTVMVANIGPASYN 324

  Fly   328 YNETLTTLRYASRAKSIQNQPIKNEDPQDAKLKEYQEEIERLKRLIGPQQQQRSEKQVTAKKQRV 392
            ..|||||||||:|||:|:|:|..||||:||.|:|:||||.|||        ::.||:...|::|.
Zfish   325 VEETLTTLRYANRAKNIKNKPRVNEDPKDALLREFQEEIARLK--------EQLEKRSGRKRRRR 381

  Fly   393 KKPKKETVTKEMSDSLQVSTIEQPVEDDSDPEGAES--ESDK----------------------- 432
            ::.:.....:|..|.      |...:||.|.|..|.  ::||
Zfish   382 RRRRVGEGGEEFEDG------EDEEDDDDDDEDEEEGVDADKNIADYWHEQQEKLEKERRAIMED 440

  Fly   433 -----ENEAEVAKSNE----ELERERVENSKLAAKLAELEGQLVRGGKNLLDTYSERQIELEKKL 488
                 |.:..:.|..|    :|.:|:..:..|.||:..:|.:|:.||||::|..:|:|..||.|.
Zfish   441 HSLVAEEKQRLLKEKERKMTDLHKEKEASEMLTAKVKAMESKLLVGGKNIVDHTNEQQKVLELKR 505

  Fly   489 VEIAERKKREIEIQQQLELQEETTLEIRERNVSLEQEVELKKRKLSKCYAKYLALQQELNDCKSD 553
            .||||:|:||.|::|::|.::|.|||::|...||:|||::|.:||.|.::|..:::.|:.|.:.:
Zfish   506 QEIAEQKRREREMKQEMECRDEETLELKETYSSLQQEVDIKTKKLKKLFSKLQSVKSEIQDAQDE 570

  Fly   554 HNQDLRELEMAQNELVKELKRQLLIIDNFVPIEVKQRLYTQAKYDEEQEEWKFSSMSLPTPPGGD 618
            |.:..:|||..||||.:|||.:.|||:||:|:|.|.::.|:|.:|||.:.||.:.:   |.....
Zfish   571 HVKYRQELEQTQNELTRELKLKHLIIENFIPMEEKNKIVTRATFDEEDDLWKMTPI---TRIQNS 632

  Fly   619 GKFSSKRPVSHPQRRRPTSEYALQEAKSNSPSSLRFKSENIVNYELEMPCRTTQEYRTPKVSASL 683
            .....|||||....|||.|::| :.|....| .:|:|:|||:..||::|.|||::|..|.::..:
Zfish   633 DHQMMKRPVSAVGYRRPLSQHA-RMAMLMRP-DVRYKAENILLLELDLPSRTTKDYEGPVIAPKV 695

  Fly   684 QAVLAQAMQTGGDDIDIVDSHTNSLRSRLENIINANANG-----GAGPGAGVAVGSSIPNVRNIK 743
            .|.|..|:                   |.|:.|..:|:|     |:.||...
Zfish   696 AAALEDAL-------------------REEDEIQVDASGFHASLGSSPGLSA------------- 728

  Fly   744 SSRGLPSAASNLDSNRRPPTGRLPAKKPASA-----------YPKARGLVNK 784
            |:.|.    |....:.||.||: ....|.||           ||::||||.|
Zfish   729 SAAGF----SKKPKSGRPKTGK-KVSTPTSAHSPLSGSGSPLYPQSRGLVPK 775

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Klp68D	NP_001261726.1	Motor_domain	18..344	CDD:277568	206/334 (62%)
		KISc	20..351	CDD:214526	208/339 (61%)
		GBP_C	<479..576	CDD:303769	43/96 (45%)
		coiled coil	547..558	CDD:293879	2/10 (20%)
kif3b	NP_001093615.1	Kinesin	15..341	CDD:278646	201/327 (61%)
		KISc_KIF3	15..341	CDD:276822	201/327 (61%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	372..419		13/52 (25%)
		GBP_C	<425..523	CDD:303769	29/97 (30%)
		coiled coil	500..511	CDD:293879	6/10 (60%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	716..775		19/76 (25%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170580426
Domainoid	1	1.000	213	1.000	Domainoid score	I2697
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H55849
Inparanoid	1	1.050	587	1.000	Inparanoid score	I956
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D145782at33208
OrthoFinder	1	1.000	-	-		FOG0002745
OrthoInspector	1	1.000	-	-		otm26374
orthoMCL	1	0.900	-	-		OOG6_101005
Panther	1	1.100	-	-	LDO	PTHR24115
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2170
SonicParanoid	1	1.000	-	-		X1829
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	13	12.930