Protein Alignment Pi3K68D and PIK3CA

DIOPT Version :10

• Sequence 1: NP_524028.2
• Gene: Pi3K68D / 39329
• FlyBaseID: FBgn0015278
• Length: 1876
• Species: Drosophila melanogaster
• Sequence 2: NP_006209.2
• Gene: PIK3CA / 5290
• HGNCID: 8975
• Length: 1068
• Species: Homo sapiens
• Alignment Length: 1217
• Identity: 305/1217 - (25%)
• Similarity: 520/1217 - (42%)
• Gaps: 242/1217 - (19%)

  Fly   455 PPPP----------LPPR-NQQCYESNQAAMPVSRPPQSSVLTDSYTSSIPANVVLDRRKTCTRL 508
            ||.|          :||| ..:|...|...:.:....:::::|      |...:..:.||  ..|
Human     2 PPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLIT------IKHELFKEARK--YPL 58

  Fly   509 YELISDQR-------TDDPELLEFYHMVK---EVRARYPHDDAPTNVGHVVAAEFNYHYMMDTSI 563
            ::|:.|:.       |.:.|..||:...:   ::|...|.                        :
Human    59 HQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPF------------------------L 99

  Fly   564 KVIVHPALNTLQSTVLAASMGKEQVKGYGMPVTFTCDIDSVVAQVVAQALASLEGQVKGTVTDYA 628
            ||| .|..|. :..:|...:|    ...||||   |:.|.|....|.        ..:..:.:..
Human   100 KVI-EPVGNR-EEKILNREIG----FAIGMPV---CEFDMVKDPEVQ--------DFRRNILNVC 147

  Fly   629 VKPIGLLEWLAPTSRLSQL--ECVHNSFQLEKDVHLGLCLSTAANMQAIARTERDDEHDADLLPE 691
            .:.:.|.:..:|.||...:  ..|.:|.:|.|.::..|.......:..:..:..:|:....|...
Human   148 KEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKIN 212

  Fly   692 H-LLPNEVV-PIVTYDNMMILIETLEMEIDKLESAADGVPGRSVVSCSGV---------VQAVKA 745
            | .:|.:|: ..:......:|:.:.::::..||     ..|:.::...|.         :...|.
Human   213 HDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLE-----YQGKYILKVCGCDEYFLEKYPLSQYKY 272

  Fly   746 I--CALLGSIDTMEIARCVADLKRICEVEQKKYSTGASNPEIVSDYGDYAQVVLRPRSMLEQIKV 808
            |  |.:||.:..:                                      :::...|:..|:.:
Human   273 IRSCIMLGRMPNL--------------------------------------MLMAKESLYSQLPM 299

  Fly   809 KCNELRDAVQELVELYANVFRVAFSVKTPDYSTTPI-PISCVSKPIVVCISCLHRPLPNWKFDDY 872
            .|..:..        |:.....|......:.||..: .|:...:..::|.:.::..:.:  .|..
Human   300 DCFTMPS--------YSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRD--IDKI 354

  Fly   873 SLCVQIVYGTRLLSKPNVLT----CSNDTSGGLFPRLNFSAWLTFDQHPICTLPREARLTFVL-- 931
            .:...|.:|...|. .||.|    |||       ||.|  .||.:|.: |..|||.|||...:
Human   355 YVRTGIYHGGEPLC-DNVNTQRVPCSN-------PRWN--EWLNYDIY-IPDLPRAARLCLSICS 408

  Fly   932 -YGKQAASEGEPNADQNGERRQVTTELGWCSIQLFDFKRVMICGPYLLSLWPPM--TDKMLGPAP 993
             .|::.|.|..             ..|.|.:|.|||:...::.|...|:|||..  .:.:|.|..
Human   409 VKGRKGAKEEH-------------CPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIG 460

  Fly   994 ARGCHPQPDFCPVLSIEVPPYGGRIEFPEHQEVPKPAP---------HYDFASL------DANLQ 1043
            ..|.:|..: .|.|.:|...:...::||:...:.:.|.         .|..|.|      |..|:
Human   461 VTGSNPNKE-TPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELR 524

  Fly  1044 EELLDTAEL-------GYTGATERREVF-WEKRLYLQSYPNALPKVLHAAHSWDYAN-LIDLHAL 1099
            |.  |..:|       ..:..||:.:.| |..|.|..:.|..|||:|.:. .|:..: :..::.|
Human   525 EN--DKEQLKAISTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSV-KWNSRDEVAQMYCL 586

  Fly  1100 LHSWAPLSPLQSLELLLPRYPDAKVREKAVEWISK-MPNDQLVDFLPQLVQSLKHDTYEGSAMAR 1163
            :..|.|:.|.|::|||...|||..||..||..:.| :.:|:|..:|.||||.||::.|..:.:.|
Human   587 VKDWPPIKPEQAMELLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVR 651

  Fly  1164 FLLSKCLESPRFAHHMYWLLVHSLPDDPHNSIGAAMVDQEYDESQVTQVRYYRRNKMMLRALMAI 1228
            |||.|.|.:.|..|..:|    .|..:.||.    .|.|.:.              ::|.:....
Human   652 FLLKKALTNQRIGHFFFW----HLKSEMHNK----TVSQRFG--------------LLLESYCRA 694

  Fly  1229 CGEKMLQRFMYQHRMCQKLTTIAESVKEAKESMRQK-SLAAGMDEVHQ-DLLE--QPTCLPLGPE 1289
            || ..|:....|....:||..:.:.:|:.|:...|| .:...::::.: |.::  |....||.|.
Human   695 CG-MYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLNPA 758

  Fly  1290 LEVTGVSVRNCSYFNSNTLPLKINFVGPDA------ESLPAIFKCGDDLQQDQLTIQLIRIMNKM 1348
            .::..:.:..|...:|...||.:|:..||.      ::...|||.||||:||.||:|:||||..:
Human   759 HQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENI 823

  Fly  1349 WLAERLDLKMVTFNCVPTGYKSGMIELVSEAETLRKIQVECGLTGS--FKDRPIAEWLGKQNPSP 1411
            |..:.|||:|:.:.|:..|...|:||:|..:.|:.:||.:.||.|:  |....:.:||..:|...
Human   824 WQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGE 888

  Fly  1412 LEYQSAVRNFTLSCAGYSVATYVLGICDRHNDNIMLKTSGHLFHIDFGKFLGDAQMFGNFKRDRT 1476
            : |.:|:..||.|||||.|||::|||.||||.|||:|..|.|||||||.||...:....:||:|.
Human   889 I-YDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERV 952

  Fly  1477 PFVLTSDMAYVINGGDKPST---DFHYFVDLCCRAFNIVRKNADLLLHTLAHMATAGMPGVNS-N 1537
            |||||.|...||:.|.:..|   :|..|.::|.:|:..:|::|:|.::..:.|..:|||.:.| :
Human   953 PFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFD 1017

  Fly  1538 AVQYVRRALLPSQSNPEAAATFAKMIQSSLK-SWFTQFNFFLHNLAQ 1583
            .:.|:|:.|...::..||...|.|.:..:.. .|.|:.::..|.:.|
Human  1018 DIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQ 1064

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pi3K68D	NP_524028.2	PHA03247	<56..294	CDD:223021
		PI3K_rbd	561..669	CDD:197540	24/109 (22%)
		C2A_PI3K_class_II	844..1021	CDD:175979	46/186 (25%)
		PI3Ka_II	1038..1202	CDD:238441	57/179 (32%)
		PI3Kc_II	1235..1583	CDD:270710	124/364 (34%)
		PX_PI3K_C2_68D	1611..1721	CDD:132794
		C2B_PI3K_class_II	1751..1871	CDD:176027
PIK3CA	NP_006209.2	PI3K_p85B	31..108	CDD:197539	17/109 (16%)
		PI3K_rbd	173..292	CDD:197540	19/161 (12%)
		C2_PI3K_class_I_alpha	325..484	CDD:176043	46/185 (25%)
		PI3Ka_I	525..696	CDD:238444	56/195 (29%)
		PI3Kc_IA_alpha	695..1064	CDD:270719	127/370 (34%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	771..777		1/5 (20%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	912..920		6/7 (86%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	931..957		13/25 (52%)