Protein Alignment Pi3K68D and Pik3ca

DIOPT Version :10

• Sequence 1: NP_524028.2
• Gene: Pi3K68D / 39329
• FlyBaseID: FBgn0015278
• Length: 1876
• Species: Drosophila melanogaster
• Sequence 2: NP_032865.2
• Gene: Pik3ca / 18706
• MGIID: 1206581
• Length: 1068
• Species: Mus musculus
• Alignment Length: 1223
• Identity: 312/1223 - (25%)
• Similarity: 519/1223 - (42%)
• Gaps: 254/1223 - (20%)

  Fly   455 PPPP----------LPPR-NQQCYESNQAAMPVSRPPQSSVLTDSYTSSIPANVVLDRRKTCTRL 508
            ||.|          :||| ..:|...|...:.:....:::::|      |...:..:.||  ..|
Mouse     2 PPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLVT------IKHELFREARK--YPL 58

  Fly   509 YELISDQR-------TDDPELLEFYHMVK---EVRARYPHDDAPTNVGHVVAAEFNYHYMMDTSI 563
            ::|:.|:.       |.:.|..||:...:   ::|...|.                        :
Mouse    59 HQLLQDETSYIFVSVTQEAEREEFFDETRRLCDLRLFQPF------------------------L 99

  Fly   564 KVIVHPALNTLQSTVLAASMGKEQVKGYGMPVTFTCDIDSVVAQVVAQALASLEGQVKGTVTDYA 628
            ||| .|..|. :..:|...:|..    .||||   |:.|.|....|.        ..:..:.:..
Mouse   100 KVI-EPVGNR-EEKILNREIGFV----IGMPV---CEFDMVKDPEVQ--------DFRRNILNVC 147

  Fly   629 VKPIGLLEWLAPTSRLSQL--ECVHNSFQLEKDVHLGLCLSTAANMQAIARTERDDEHDADLLPE 691
            .:.:.|.:..:|.||...:  ..|.:|.:|.|.::..|.......:..:..:..:|:....|...
Mouse   148 KEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKIN 212

  Fly   692 H-LLPNEVV-PIVTYDNMMILIETLEMEIDKLESAADGVPGRSVVSCSGV---------VQAVKA 745
            | .:|.:|: ..:......:|:.:.::::..||     ..|:.::...|.         :...|.
Mouse   213 HDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLE-----YQGKYILKVCGCDEYFLEKYPLSQYKY 272

  Fly   746 I--CALLGSIDTMEIARCVADLKRICEVEQKKYS-------TGASNPEIVSDYGDYAQVVLRPRS 801
            |  |.:||.:..:.:.           .::..||       |..|....:|....|    :...:
Mouse   273 IRSCIMLGRMPNLMLM-----------AKESLYSQLPIDSFTMPSYSRRISTATPY----MNGET 322

  Fly   802 MLEQIKVKCNELRDAVQELVELYANVFRVAFSVKTPDYSTTPIPISCVSKPIVVCISCLHRPLPN 866
            ..:.:.|..:.||  ::.|...|.||     :::..|             .|.|.....|...|
Mouse   323 STKSLWVINSALR--IKILCATYVNV-----NIRDID-------------KIYVRTGIYHGGEP- 366

  Fly   867 WKFDDYSLCVQIVYGTRLLSKPNVLT----CSNDTSGGLFPRLNFSAWLTFDQHPICTLPREARL 927
                   ||            .||.|    |||       ||.|  .||.:|.: |..|||.|||
Mouse   367 -------LC------------DNVNTQRVPCSN-------PRWN--EWLNYDIY-IPDLPRAARL 402

  Fly   928 TFVL---YGKQAASEGEPNADQNGERRQVTTELGWCSIQLFDFKRVMICGPYLLSLWPPM--TDK 987
            ...:   .|::.|.|..             ..|.|.:|.|||:...::.|...|:|||..  .:.
Mouse   403 CLSICSVKGRKGAKEEH-------------CPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLED 454

  Fly   988 MLGPAPARGCHPQPDFCPVLSIEVPPYGGRIEFPEHQEVPKPAP---------HYDFASL----- 1038
            :|.|....|.:|..: .|.|.:|...:...::||:...:.:.|.         .|....|
Mouse   455 LLNPIGVTGSNPNKE-TPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHTGLSNRLA 518

  Fly  1039 -DANLQEELLDTAEL-------GYTGATERREVF-WEKRLYLQSYPNALPKVLHAAHSWDYAN-L 1093
             |..|:|.  |..:|       ..:..||:.:.| |..|.|..:.|..|||:|.:. .|:..: :
Mouse   519 RDNELREN--DKEQLRALCTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSV-KWNSRDEV 580

  Fly  1094 IDLHALLHSWAPLSPLQSLELLLPRYPDAKVREKAVEWISK-MPNDQLVDFLPQLVQSLKHDTYE 1157
            ..::.|:..|.|:.|.|::|||...|||..||..||..:.| :.:|:|..:|.||||.||::.|.
Mouse   581 AQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRSFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYL 645

  Fly  1158 GSAMARFLLSKCLESPRFAHHMYWLLVHSLPDDPHNSIGAAMVDQEYDESQVTQVRYYRRNKMML 1222
            .:.:.||||.|.|.:.|..|..:|    .|..:.||.    .|.|.:.              ::|
Mouse   646 DNLLVRFLLKKALTNQRIGHFFFW----HLKSEMHNK----TVSQRFG--------------LLL 688

  Fly  1223 RALMAICGEKMLQRFMYQHRMCQKLTTIAESVKEAKESMRQK-SLAAGMDEVHQ-DLLE--QPTC 1283
            .:....|| ..|:....|....:||..:.:.:|:.|:...|| .:...::::.| |.::  |...
Mouse   689 ESYCRACG-MYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRQPDFMDALQGFL 752

  Fly  1284 LPLGPELEVTGVSVRNCSYFNSNTLPLKINFVGPDA------ESLPAIFKCGDDLQQDQLTIQLI 1342
            .||.|..::..:.:..|...:|...||.:|:..||.      ::...|||.||||:||.||:|:|
Mouse   753 SPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQII 817

  Fly  1343 RIMNKMWLAERLDLKMVTFNCVPTGYKSGMIELVSEAETLRKIQVECGLTGS--FKDRPIAEWLG 1405
            |||..:|..:.|||:|:.:.|:..|...|:||:|..:.|:.:||.:.||.|:  |....:.:||.
Mouse   818 RIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLK 882

  Fly  1406 KQNPSPLEYQSAVRNFTLSCAGYSVATYVLGICDRHNDNIMLKTSGHLFHIDFGKFLGDAQMFGN 1470
            .:|...: |.:|:..||.|||||.|||::|||.||||.|||:|..|.|||||||.||...:....
Mouse   883 DKNKGEI-YDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFG 946

  Fly  1471 FKRDRTPFVLTSDMAYVINGGDKPST---DFHYFVDLCCRAFNIVRKNADLLLHTLAHMATAGMP 1532
            :||:|.|||||.|...||:.|.:..|   :|..|.::|.:|:..:|::|:|.::..:.|..:|||
Mouse   947 YKRERVPFVLTQDFLIVISKGAQEYTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMP 1011

  Fly  1533 GVNS-NAVQYVRRALLPSQSNPEAAATFAKMIQSSLK-SWFTQFNFFLHNLAQ 1583
            .:.| :.:.|:|:.|...::..||...|.|.:..:.. .|.|:.::..|.:.|
Mouse  1012 ELQSFDDIAYIRKTLALDKTEQEALEYFTKQMNDAHHGGWTTKMDWIFHTIKQ 1064

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pi3K68D	NP_524028.2	PHA03247	<56..294	CDD:223021
		PI3K_rbd	561..669	CDD:197540	24/109 (22%)
		C2A_PI3K_class_II	844..1021	CDD:175979	46/185 (25%)
		PI3Ka_II	1038..1202	CDD:238441	57/179 (32%)
		PI3Kc_II	1235..1583	CDD:270710	125/364 (34%)
		PX_PI3K_C2_68D	1611..1721	CDD:132794
		C2B_PI3K_class_II	1751..1871	CDD:176027
Pik3ca	NP_032865.2	PI3K_p85B	31..108	CDD:197539	17/109 (16%)
		PI3K_rbd	173..292	CDD:197540	19/134 (14%)
		C2_PI3K_class_I_alpha	325..484	CDD:176043	54/222 (24%)
		PI3Ka_I	525..696	CDD:238444	56/195 (29%)
		PI3Kc_IA_alpha	695..1064	CDD:270719	128/370 (35%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	771..777		1/5 (20%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	912..920		6/7 (86%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	931..957		13/25 (52%)