DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ube3a and HERC2

DIOPT Version :9

Sequence 1:	NP_648452.1	Gene:	Ube3a / 39266	FlyBaseID:	FBgn0061469	Length:	973	Species:	Drosophila melanogaster
Sequence 2:	NP_608388.2	Gene:	HERC2 / 33035	FlyBaseID:	FBgn0031107	Length:	4912	Species:	Drosophila melanogaster

Alignment Length:	1162	Identity:	241/1162 - (20%)
Similarity:	383/1162 - (32%)	Gaps:	374/1162 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly    10 DQHLPGVSSASGSAA--GAVGG------------------------RATPEMKRSAVRSLIKRYF 48
            |..||..||:|.|:|  ||:..                        |.| |:.|:....:.|::.
  Fly  3887 DAALPATSSSSSSSAAPGAISSTGDVHKWAWFKRYCIAVRVAQSLIRRT-ELPRAFCLEVRKKFA 3950

  Fly    49 HQLQSGCGNANCSNANCASSG-------------KVAPMTP--------NEVAARALQLFSQDAQ 92
            ..|.|...|:| :|..|.|.|             .|:.::|        .::...|.||.|....
  Fly  3951 EMLPSSSSNSN-ANPGCQSPGASMLNSTTSLSSSTVSNVSPPPGITGEQPDLHCHAHQLESTSTL 4014

  Fly    93 LCEAFTSEANSPQDVDMLS-----PNDSSSSSSGSSTSTITSASTTTTTSRQSQSTPAAVVVPVS 152
            |.|..|. ..:|.|..:|.     |:|.:.|..|:||
  Fly  4015 LHEDHTL-FQAPHDAQLLQWLNRRPDDWALSWGGAST---------------------------- 4050

  Fly   153 SPYLTQSVPQLDIAGAEHSS----GDSEPC---TPTLAPVHSLDANSLIALYEQCRAADSYDRIC 210
                        |.|..|:.    |..|..   |||.....||.....:|..||...|.:.|...
  Fly  4051 ------------IYGWGHNHRGQLGGLEGSRIKTPTPCEALSLLRPVQLAGGEQSLFAVTPDGKL 4103

  Fly   211 HAIGVVFSSVDRLGKSFIRS-AEPAASSSLQ-----ELLANSPG----ALNKEQLRTLEGE---- 261
            .|.|  :.|..|||.....| |.|....|||     ::..||.|    ||      |.|||
  Fly  4104 FATG--YGSGGRLGVGGSDSWAIPTLLGSLQHVFVKKVAVNSGGKHCLAL------TTEGEVYAW 4160

  Fly   262 ---------------HDKDE-----DSTQQVDEQSESAANATATAE-----------------SV 289
                           :|:.:     :.....|....||.:|..||.                 ..
  Fly  4161 GEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDS 4225

  Fly   290 ESEAEPEEED--------DDVCSSQSSDTLV-------------DLPGLRRVQRLLFGCQIRAIT 333
            |.:..|:..:        |..|.|..:.||.             |...|.|...  .||::....
  Fly  4226 EDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGS--DGCKLPYKI 4288

  Fly   334 EKLTS------------SVIQLSDWVIYM--RTDWERVIHCLVICFDLATNTNNSVVDMDYLDRV 384
            |.|..            ||.......:|.  :.|:.|:.|..|                |::.|
  Fly  4289 ESLAGLGVVKVECGSQFSVALTKSGAVYTWGKGDFHRLGHGSV----------------DHVRR- 4336

  Fly   385 LPKLCQAASAMPVPAQARLARIWAAHCSDQLHSLIAACQQQITLQVLLDEESMRENESIISVTKV 449
             ||...|.....:.:.|    ..:.||        .||.....:....|.:..:..:..::.
  Fly  4337 -PKKVAALQGKKIISIA----TGSLHC--------VACSDSGEVYTWGDNDEGQLGDGTVTA--- 4385

  Fly   450 LKIVFYANILASELERPSCRVPLEDRTEAATASGSAAVEDDLFVYNSLLEPHMPKFAEDQFEKEL 514
                         ::||.....|:.:.......|||               |....:..|..:.|
  Fly  4386 -------------IQRPRLVAALQGKHIVKVTCGSA---------------HTLALSTSQLSERL 4422

  Fly   515 QVSAIDCRKPL--IPLE-----EFYNEAL-SENIQMHH-DYLSYKTLAMESEIGSGHANYFSFML 570
                    :||  .|||     :...||| :..|.:|| ..|....|||....|.
  Fly  4423 --------RPLPNPPLEYDLVRDLAPEALHARLILLHHFSELVCPCLAMLPISGD---------- 4469

  Fly   571 YAFILTPSTKVDALYYDSRMRMYSERYSSLHSILNNIGQVGQEDNPRPDL-KLTVRRDQLINDAL 634
                |:.....|.|       :|:.:.::...::.......::..|..:| ::.|:|.:...:.|
  Fly  4470 ----LSLGALKDVL-------VYNIKEAAFRKVIQTTMVRDKQHGPVIELNRIQVKRSRNRCNGL 4523

  Fly   635 IGL--------EMV---------AMSNPKDLKKQLVVEFVGEQGIDEGGVSKEFFQLIVEEIFNP 682
            .|:        :||         |::.|..:.|   |:||||...|.||...|....:.:|:.|.
  Fly  4524 AGIDGMKSVFGQMVQKLPLLTQEALALPHRVWK---VKFVGESVDDCGGGYSESIAEMCDELQNG 4585

  Fly   683 AFGMFIQ------QEETNNMWFNATPFENGA----QFTLIGIIIGLAIYNNVTLAVNFPMVVYRK 737
            :..:.|.      :...|...|...|..:..    .|..:|:::|:|:.....|::|....|:|:
  Fly  4586 SVPLLINTPNGRGEAGANRDCFLLDPTLSSVLQMNMFRFLGVLMGIAVRTGSPLSINLAEPVWRQ 4650

  Fly   738 LIGYCGTFADLS----------------DWSPALYKSLKSMLDYQGQDMEEVFEQTFKISYSDVF 786
            |.|......||:                |..|.|:.:|:                 ...|.|...
  Fly  4651 LTGEVLRPTDLTEVDRDYVAGLLCIRNMDDDPKLFTALE-----------------LPFSTSSAR 4698

  Fly   787 GDVVQHELVPNGQDVLVGQHNKELFVNLYSDFLLNTNIEQQFNAFRKGFEMVTDESPLKLLFRPE 851
            |    ||:..:.:...:...|:..:|.|...|.|: ..::|..|.|.|...|. ..||..||...
  Fly  4699 G----HEVPLSTRYTHISPRNRAEYVRLALGFRLH-EFDEQVKAVRDGMSKVI-PVPLLSLFSAA 4757

  Fly   852 EIEMLVCGSREFDFVELENSTVYEGGYTEKSQYIQDFWSIVHAMPSEDKHKLLEFTTGSARVP-- 914
            |::.:||||.:.....|::...|: |:...|..:..||.::....::::...|.|..|..|:|
  Fly  4758 ELQAMVCGSPDIPLGLLKSVATYK-GFDPSSALVTWFWEVMEEFTNQERSLFLRFVWGRTRLPRT 4821

  Fly   915 ---VGGLKCLRLLITRHGPDSDRLPTSHTCFNVLLLPEYSSREKLEERLMKAINYSK 968
               ..|...:..::.::.|| ..||.|:|||.:|.:|.||.:..|.|:|..||::.|
  Fly  4822 IADFRGRDFVLQVLEKNPPD-HFLPESYTCFFLLKMPRYSCKAVLLEKLKYAIHFCK 4877

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ube3a	NP_648452.1	AZUL	40..94	CDD:293166	15/74 (20%)
		HECTc	620..971	CDD:238033	95/398 (24%)
		HECTc	647..970	CDD:214523	86/353 (24%)
HERC2	NP_608388.2	RCC1	638..683	CDD:278826
		RCC1	687..737	CDD:278826
		RCC1	740..787	CDD:278826
		RCC1	790..841	CDD:278826
		RCC1_2	828..857	CDD:290274
		Cyt-b5	1290..1363	CDD:278597
		MIB_HERC2	1928..1987	CDD:284184
		UBA_HERC2	2515..2559	CDD:270585
		Cul7	2624..2699	CDD:288381
		APC10-HERC2	2762..2932	CDD:176485
		RCC1	3037..3088	CDD:278826
		RCC1	3091..3140	CDD:278826
		RCC1	3144..3194	CDD:278826
		RCC1	3197..3246	CDD:278826
		RCC1	3250..3298	CDD:278826
		RCC1	3302..3350	CDD:278826
		RCC1_2	4136..4166	CDD:290274	9/35 (26%)
		RCC1	4154..4203	CDD:278826	8/48 (17%)
		RCC1	4206..4257	CDD:278826	7/50 (14%)
		RCC1	4260..4309	CDD:278826	9/50 (18%)
		RCC1	4312..4360	CDD:278826	13/77 (17%)
		RCC1	4364..4413	CDD:278826	8/79 (10%)
		HECTc	4509..4880	CDD:238033	94/397 (24%)
		HECTc	4553..4878	CDD:214523	86/353 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45446967
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm3500
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	4	3.740