DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ube3a and Hecw2

DIOPT Version :9

Sequence 1:	NP_648452.1	Gene:	Ube3a / 39266	FlyBaseID:	FBgn0061469	Length:	973	Species:	Drosophila melanogaster
Sequence 2:	NP_001001883.1	Gene:	Hecw2 / 329152	MGIID:	2685817	Length:	1578	Species:	Mus musculus

Alignment Length:	1259	Identity:	269/1259 - (21%)
Similarity:	454/1259 - (36%)	Gaps:	411/1259 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly    14 PGVSSASGSAAGAVGGRATPEMKRS-----AVRSLI----KRYFHQLQSGCGNAN---------- 59
            ||.:|:|..:.|     |:|:::.|     .:.:::    ....|:.|...|..:
Mouse   430 PGAASSSERSMG-----ASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIM 489

  Fly    60 CSNANCASSGKVA----PMTPNEVAARALQLFSQDAQLCEAFT---------------------- 98
            ||.|:....|.:.    |...|.|.       ::||.:.|..:
Mouse   490 CSRASRIDDGSLTSQTKPEDDNPVE-------NEDASIHETASLEERLENLPEVADGSLPSSTAP 547

  Fly    99 --SEAN---------------SPQDVDMLSPNDSSSSSSGSSTSTITSASTTTTTSRQSQSTPAA 146
              :|||               |.|:||. ..:.:.:.:|.:|..:..:||.|.:..:.|:.:..:
Mouse   548 DENEANLEPQPSADQGSTELCSSQEVDQ-PTSGADAGASDTSGGSRRAASETESLDQGSEPSQVS 611

  Fly   147 VVVPVSSPYLTQSVPQL--------DIAGAEHSSGDSEPCTPTLAPVHSLDANSLIALYEQCRAA 203
            .....|.|..|:||.:.        |..||:.|..:|  .|..|:.|.:..::...|.:.:..|.
Mouse   612 SETEPSDPARTESVSEASTRPEGESDPEGADSSCNES--VTTQLSSVETRCSSLESARFPETPAF 674

  Fly   204 DSY---DRICHAIGVVFSSVDRLGKSFIRSAEPAAS---SSLQELLANSPGALNKEQLRTLEGEH 262
            .|.   |..|                   :|||.:|   ...||.:. :|.:|...|:.:.|.|.
Mouse   675 SSQEEEDGAC-------------------AAEPTSSGPAEGSQESVC-TPSSLPAVQVPSREEEG 719

  Fly   263 DKDEDSTQQVDEQSE-------------SAANATATAESVESEAEPEEEDD-----DVC------ 303
            ...|.:.  :.||.|             :||.|.|.|   .::::|:|:.|     ..|
Mouse   720 SAAEAAA--LSEQGELGEVWQRRGSLEGAAAAAPAAA---ATDSQPQEDGDAGDAQGACEGATAQ 779

  Fly   304 ------SSQSS--DTLVDLPGLRR-VQRLLFGCQIRAITEKLTSSVIQLSDWVIYMRTD------ 353
                  .||::  ..|..||.:|: |.|                          |.|.|
Mouse   780 EEGATGGSQTNGHQPLRSLPSVRQDVSR--------------------------YQRVDEALPPN 818

  Fly   354 WERVI--HCLVICFDLATNT-----------------NNSVVDMDYLDR---------------- 383
            ||..|  |..:...|....|                 :||:..|:.|:|
Mouse   819 WEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEE 883

  Fly   384 -------------------------VLP------KLCQAASAMPVPAQARLARIWAAHCSDQLHS 417
                                     |||      :|.....:.||............|.:...:.
Mouse   884 NTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYR 948

  Fly   418 LIA--ACQQQITLQVLLDE---ESMRENESIISVTKVLKIVFYANILAS-ELERP---------- 466
            :..  .|.:.:..:|..|.   |..:.|..::.         :.|:.|: :||.|
Mouse   949 MFTNNTCLKHMITKVRRDTHHFERYQHNRDLVG---------FLNMFANKQLELPRGWEMKHDHQ 1004

  Fly   467 ----------------SCRVPLEDR------------TEAATASGSAAVEDDLF----------- 492
                            ..|:||:..            |...:.|.....||...
Mouse  1005 GKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSS 1069

  Fly   493 VYNSLLEPHMPKFAEDQFEKELQVSAID-----CRKP--LIPLEE-----FYNEALSENIQM--- 542
            .:|::..|        |::..:.|:..|     .|:|  |..|:|     ..|.:|.|.||.
Mouse  1070 TFNTVSRP--------QYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRT 1126

  Fly   543 -----------HHDYLSYKTLAMESEIGSG-------HANYFSFMLYAFILTP---------STK 580
                       ..|.:...:| .|.||.|.       |.:|......:.:.:|         :.:
Mouse  1127 EGTPGLVRLSSDADLVMLLSL-FEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANAR 1190

  Fly   581 VDALY---YDSRMRMYSERYSSLHSILNNIGQVGQEDNPRPDLKLTVRRDQLINDALIGLEMVAM 642
            ..|.|   :::::|.:   |..|.:       .|....| ..|||.:|||.|:.||   ...:..
Mouse  1191 APAPYKRDFEAKLRNF---YRKLET-------KGYGQGP-GKLKLIIRRDHLLEDA---FNQIMG 1241

  Fly   643 SNPKDLKK-QLVVEFVGEQGIDEGGVSKEFFQLIVEEIFNPAFGMFIQQEETNNMW------FNA 700
            .:.|||:: :|.|.||||:|:|..|.|:|||.|:..|:|||.:|:|  :...|:.:      .:|
Mouse  1242 YSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLF--EYSANDTYTVQISPMSA 1304

  Fly   701 TPFENGAQFTLIGIIIGLAIYNNVTLAVNFPMVVYRKLIGYCGTFADLSDWSPALYKSLKSMLDY 765
            ....:...|...|.|:|||:.:...|...|....|:.|:......:||.......::||:.|.|
Mouse  1305 FVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKD- 1368

  Fly   766 QGQDMEEVFEQTFKISYSDVFGDVVQHELVPNGQDVLVGQHNKELFVNLYSDFLLNTNIEQQFNA 830
              .|:.::.:.||.:: .:|||.:.:.||.|.|.::.|.:.||:.::.....:.:...:.||..:
Mouse  1369 --NDIHDILDLTFTVN-EEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTES 1430

  Fly   831 FRKGFEMVTDESPLKLLFRPEEIEMLVCGSREFDFVELENSTVYEGGYTEKSQYIQDFWSIVHAM 895
            ..:||..|.| :.|..:|...|:|:::.|:.|.|..:..|:|.|.|||.:....|:.||:.|...
Mouse  1431 LVRGFYEVVD-ARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERF 1494

  Fly   896 PSEDKHKLLEFTTGSARVPVGGLKCLRLLITRHGPDSDR------------LPTSHTCFNVLLLP 948
            .:|.:.:||:|.||::.:|..|...||      |.:..|            ||.:|||||.|.||
Mouse  1495 NNEQRLRLLQFVTGTSSIPYEGFASLR------GSNGPRRFCVEKWGKITALPRAHTCFNRLDLP 1553

  Fly   949 EYSSREKLEERLMKAINYSKGFGM 972
            .|.|...|.|:|:.|:..:..||:
Mouse  1554 PYPSFSMLYEKLLTAVEETSTFGL 1577

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ube3a	NP_648452.1	AZUL	40..94	CDD:293166	12/71 (17%)
		HECTc	620..971	CDD:238033	114/369 (31%)
		HECTc	647..970	CDD:214523	104/341 (30%)
Hecw2	NP_001001883.1	HECW_N	45..164	CDD:374632
		C2_NEDL1-like	185..320	CDD:176073
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	341..453		8/27 (30%)
		DMP1	<344..700	CDD:369294	57/303 (19%)
		NESP55	<463..639	CDD:115071	32/183 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	496..802		67/340 (20%)
		Interaction with TP73. /evidence=ECO:0000250	737..1074		56/374 (15%)
		WW	815..844	CDD:366073	6/28 (21%)
		HECW1_helix	921..987	CDD:375862	9/74 (12%)
		WW	993..1022	CDD:366073	1/28 (4%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1030..1075		5/44 (11%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1167..1193		1/25 (4%)
		HECTc	1222..1576	CDD:238033	114/369 (31%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810