DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ube3a and HECTD1

DIOPT Version :9

Sequence 1:	NP_648452.1	Gene:	Ube3a / 39266	FlyBaseID:	FBgn0061469	Length:	973	Species:	Drosophila melanogaster
Sequence 2:	NP_056197.3	Gene:	HECTD1 / 25831	HGNCID:	20157	Length:	2610	Species:	Homo sapiens

Alignment Length:	1317	Identity:	261/1317 - (19%)
Similarity:	465/1317 - (35%)	Gaps:	430/1317 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly    31 ATPEMKRSAVRSLIKRYFHQLQSGCGN-----ANCSNANCASSGKVAPMTPNEVAARALQLFSQD 90
            |:|:...|.|....:.:...:::.|.:     |..|:...:||...:..:.::::..:.:...:.
Human  1344 ASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRS 1408

  Fly    91 AQLCE-AFTSEANSPQDVDMLS-----PNDSSSSSSGSSTSTITSASTTTTTSRQ-----SQSTP 144
            ..:.| :..|.|:..:.:.:||     |.....|||.:||||:|:.:.:....|:     |..||
Human  1409 EIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTP 1473

  Fly   145 ---AAV---VVPVSSPYLTQ-----------------------SVPQLDIAGAEHSSGDSEPCTP 180
               :|:   :|.||||.::.                       ||..|..|||..||..|.|
Human  1474 GESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVP--- 1535

  Fly   181 TLAPVHSLDANSL------IALYEQCRAAD-------SYDRICHAIG--VVFSSVDRL--GKSFI 228
               .:.|.:.:||      :|...:..|.:       |.|...:.:|  |:.::...|  .:||.
Human  1536 ---NLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFP 1597

  Fly   229 RSAEPAASSSL---------QELLANSPGALNKEQ---------LRTLEGEHDKDEDSTQQVDEQ 275
            ....|..:|::         ...:|.:...|:..|         |.:...|.|..:::...:.:.
Human  1598 NLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDF 1662

  Fly   276 SESAANATATAESVESE--AEPEEEDDDVCSSQSSD------TLVDLPGLRRVQRLLFGCQIRAI 332
            .:|...:|..||..:.|  .||:||||:.......|      .::..|.|:|...........|:
Human  1663 LDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAV 1727

  Fly   333 TEKLT-------SSVIQLSDWVIY-----MRTDWE------RVIHCLVICFDLATNTNN--SVVD 377
            |.:|.       |..|...:...|     .|..|:      |....||..||......|  ...|
Human  1728 TSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTD 1792

  Fly   378 MDYLDRVLP--KLCQAASAMPVPAQA--------------------------------------- 401
            ::......|  :|.:.....|.|..|
Human  1793 LEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNG 1857

  Fly   402 -----RLARIW---------------------------AAHC-----SDQL--HSLIAACQQQIT 427
                 :|.|||                           ..|.     :|:|  :.||...|:...
Human  1858 NVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNAD 1922

  Fly   428 LQVLL------DEESMRENES---IISVTK--------------VLKIVFYANILASELERPSCR 469
            ...|.      ..:|:|:|.:   :|:..|              .:..:..::||....|:|
Human  1923 AAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQP--- 1984

  Fly   470 VPLEDRTEAATASGSAAVEDDL----FVYNSLLEPHMPKFAEDQFEKELQVSAIDCRKPLIPLEE 530
                 :.:|.....|..|||.|    .:|....:|: .:.:::..:::.|.:        .|.:|
Human  1985 -----QAKAGNGQNSCGVEDVLQLLRILYIVASDPY-SRISQEDGDEQPQFT--------FPPDE 2035

  Fly   531 FYNEALSENIQMHHDYLSYKTLAMESEIGSGHANYFSFMLYA---FILTPSTKVDALYY------ 586
            |.::.::..|....:    :.||    :.||....:...|.:   |::...|:  .||:
Human  2036 FTSKKITTKILQQIE----EPLA----LASGALPDWCEQLTSKCPFLIPFETR--QLYFTCTAFG 2090

  Fly   587 --------DSRMRMYSERYSSLHSILNN------IGQVGQEDNPRPDLKLTVRRDQLINDALIGL 637
                    .:|.....||..:..|:..:      :|::..|       ::.|.|    .::|:..
Human  2091 ASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHE-------RVKVPR----GESLMEW 2144

  Fly   638 EMVAMSNPKDLKKQLVVEFVGEQGIDEGGVSKEFFQLIVEEIFNPAFGMFI-----QQEETNNM- 696
            ....|....|.|..|.|||:||:|... |.:.||:.|:..|......|.::     ..:|:.::
Human  2145 AENVMQIHADRKSVLEVEFLGEEGTGL-GPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVD 2208

  Fly   697 -----------------WFNATPFENGAQ--------FTLIGIIIGLAIYNN--VTLAVNFPM-- 732
                             .|.| ||...:.        |..:||.:...|.:|  |.|.::.|.
Human  2209 LGGGLKPPGYYVQRSCGLFTA-PFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFK 2272

  Fly   733 -------------VVY-----RKLIGYC------------------GTFADLSDWS--------- 752
                         ::|     |.|  :|                  |:|.:.|...
Human  2273 LMCMGDIKSNMSKLIYESRGDRDL--HCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPK 2335

  Fly   753 -PALYKSLKSMLDYQ------GQDMEEVFEQTFK------------------------------- 779
             ||.:..:.:..|::      .:.::|:.:...|
Human  2336 PPAWFNGILTWEDFELVNPHRARFLKEIKDLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSG 2400

  Fly   780 --ISYSD------------VFGDVVQHELVPNGQDVLVGQHNKELFVNLYSDFLLNTNIEQQFNA 830
              :|..|            ::| ....:|.|:|:|.::...|.|.:|:|..||.::|.|::|..|
Human  2401 PPLSIEDLGLNFQFCPSSRIYG-FTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEA 2464

  Fly   831 FRKGFEMVTDESPLKLL--FRPEEIEMLVCGSREFDFV--ELENSTVYEGGYTEKSQYIQDFWSI 891
            ||.||..|   .|::.|  |..||::|::||::...:.  ::.|.|..:.|||..|.....|..:
Human  2465 FRDGFNKV---FPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRV 2526

  Fly   892 VHAMPSEDKHKLLEFTTGSARVPVGGLKCL--RLLITRHGPDSD-RLPTSHTCFNVLLLPEYSSR 953
            :..|.|:::...|:||||.:.:|.|||..|  ||.:.|....:| ..|:.:||.:.|.||||||.
Human  2527 LCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSE 2591

  Fly   954 EKLEERLMKAINYSKGF 970
            |.:.|||: |....|||
Human  2592 EIMRERLL-AATMEKGF 2607

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ube3a	NP_648452.1	AZUL	40..94	CDD:293166	6/58 (10%)
		HECTc	620..971	CDD:238033	115/490 (23%)
		HECTc	647..970	CDD:214523	109/461 (24%)
HECTD1	NP_056197.3	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	246..269
		Ank_2	366..457	CDD:403870
		ANK repeat	366..393	CDD:293786
		ANK 1	395..424
		ANK repeat	396..426	CDD:293786
		ANK 2	426..455
		ANK repeat	428..457	CDD:293786
		ANK 3	459..491
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	489..513
		ANK 4	579..612
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	627..657
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	707..748
		Sad1_UNC	1107..1240	CDD:400199
		MIB_HERC2	1277..1335	CDD:399589
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1343..1406		9/61 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1433..1483		14/49 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1496..1515		0/18 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1592..1611		4/18 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1674..1757		18/82 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1777..1797		4/19 (21%)
		BTHB	1892..1965	CDD:408209	12/72 (17%)
		K-box	2029..2103		14/91 (15%)
		HECTc	2131..2608	CDD:238033	116/497 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2297..2318		1/20 (5%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.900