Protein Alignment CalpB and CAPN13

DIOPT Version :10

• Sequence 1: NP_524016.4
• Gene: CalpB / 39165
• FlyBaseID: FBgn0025866
• Length: 925
• Species: Drosophila melanogaster
• Sequence 2: NP_653176.2
• Gene: CAPN13 / 92291
• HGNCID: 16663
• Length: 669
• Species: Homo sapiens
• Alignment Length: 750
• Identity: 226/750 - (30%)
• Similarity: 345/750 - (46%)
• Gaps: 113/750 - (15%)

  Fly   204 EEPSVGVAELSFTSVKVPENQNMFWMGRKATSARQNSVSKGDFQSLRDSCLANGTMFEDPDFPAT 268
            :||||..:.:.|      ::|                    ||.:|||.||:.|..|:|..|||.
Human     5 QEPSVETSIIKF------KDQ--------------------DFTTLRDHCLSMGRTFKDETFPAA 43

  Fly   269 NAS----LMYSRRPDRYYEWLRPGDI-ADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDST 328
            ::|    |:..:|..... |.||.|: ...|.|.::..||||:|||...|||.|||..:|||:..
Human    44 DSSIGQKLLQEKRLSNVI-WKRPQDLPGGPPHFILDDISRFDIQQGGAADCWFLAALGSLTQNPQ 107

  Fly   329 LFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKN-EFWSALLEK 392
            ...:::.. |.|...|||||.|:|||.|:|||||||||||....:.:::....:| |||..||||
Human   108 YRQKILMV-QSFSHQYAGIFRFRFWQCGQWVEVVIDDRLPVQGDKCLFVRPRHQNQEFWPCLLEK 171

  Fly   393 AYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDIKEAPPNLFSIMMKAAERGSMMGCSLEPDPHV 457
            |||||.|||..|..|...:|:.|.||||.....:..:|.:|...:..|.:.||::.|:....|..
Human   172 AYAKLLGSYSDLHYGFLEDALVDLTGGVITNIHLHSSPVDLVKAVKTATKAGSLITCATPSGPTD 236

  Fly   458 LEAETPQGLIRGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWG-NDAEWSGPWSDSSPEW---- 517
            .......||:..|||::|....:..    |:|...:|.:.|||| .:|||.|.|||.|.||
Human   237 TAQAMENGLVSLHAYTVTGAEQIQY----RRGWEEIISLWNPWGWGEAEWRGRWSDGSQEWEETC 297

  Fly   518 --RFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFE 580
              |....|.|.|       |||||||.|||...|..:.||:..|  :|.|..::....|...||.
Human   298 DPRKSQLHKKRE-------DGEFWMSCQDFQQKFIAMFICSEIP--ITLDHGNTLHEGWSQIMFR 353

  Fly   581 GEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTI 645
            .:...|.||||.||       :.|:..|:::|.:..:...|.. ||:...|.:::.
Human   354 KQVILGNTAGGPRN-------DAQFNFSVQEPMEGTNVVVCVT-VAVTPSNLKAED--------- 401

  Fly   646 GFAIYHLTDRDMQVKPQGLNFFKYRASVARSPHFINT---------REVCARFKLPPGHYLIVPS 701
              |.:.|   |.||...|...|:.:........|.||         |.....:.|.||:|::|..
Human   402 --AKFPL---DFQVILAGSQRFREKFPPVFFSSFRNTVQSSNNKFRRNFTMTYHLSPGNYVVVAQ 461

  Fly   702 TFDPNEEGEFIIRVFSETRNNMEENDDEVGFGETDDRIAPSLPPPTPKEEDDPQRIALRRLFDSV 766
            |  ..:..||::|:|.:    |.::|..:. ...:.|:..|     |.|....|.|     |:..
Human   462 T--RRKSAEFLLRIFLK----MPDSDRHLS-SHFNLRMKGS-----PSEHGSQQSI-----FNRY 509

  Fly   767 AGSDEEVDWQELKRILDHSMRDVMVGSDG--FSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIA 829
            |....::|..:|:.:|:   ::::.|..|  ||.|..||:||:::...:|||..|||..|...:.
Human   510 AQQRLDIDATQLQGLLN---QELLTGPPGDMFSLDECRSLVALMELKVNGRLDQEEFARLWKRLV 571

  Fly   830 KWRAVFKLYDTRRTGSIDGFHLRGALNSA----GYHLNNRLLNALAHRYGSREGQIPFDDFLMCA 890
            .::.||:...| ..|.:....|..|:.:.    |..::..||:.:..||....|::.|...:...
Human   572 HYQHVFQKVQT-SPGVLLSSDLWKAIENTDFLRGIFISRELLHLVTLRYSDSVGRVSFPSLVCFL 635

  Fly   891 IKVRTFIEMFRERDTDNSDTAFFNLDDWLERTIYS 925
            :::....:.||....|........: :|:...:|:
Human   636 MRLEAMAKTFRNLSKDGKGLYLTEM-EWMSLVMYN 669

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CalpB	NP_524016.4	PHA03247	<40..207	CDD:223021	1/2 (50%)
		Peptidase_C2	260..556	CDD:459889	123/308 (40%)
		Calpain_III	579..716	CDD:460050	35/145 (24%)
		EFh_PEF_CalpA_B	758..925	CDD:320071	38/172 (22%)
		EF-hand motif	758..786	CDD:320071	5/27 (19%)
		EF-hand motif	799..829	CDD:320071	12/29 (41%)
		EF-hand motif	830..860	CDD:320071	6/33 (18%)
		EF-hand motif	866..894	CDD:320071	6/27 (22%)
		EF-hand motif	895..925	CDD:320071	4/29 (14%)
CAPN13	NP_653176.2	Peptidase_C2	35..331	CDD:459889	123/308 (40%)
		Calpain_III	347..474	CDD:469636	37/150 (25%)
		EFh_PEF_CAPN13_14	501..669	CDD:320070	40/177 (23%)
		EF-hand motif	501..529	CDD:320070	7/35 (20%)
		EF-hand motif	542..571	CDD:320070	11/28 (39%)
		EF-hand motif	572..605	CDD:320070	6/33 (18%)
		EF-hand motif	611..639	CDD:320070	6/27 (22%)
		EF-hand motif	641..669	CDD:320070	4/28 (14%)