Protein Alignment CalpB and capn2b

DIOPT Version :10

• Sequence 1: NP_524016.4
• Gene: CalpB / 39165
• FlyBaseID: FBgn0025866
• Length: 925
• Species: Drosophila melanogaster
• Sequence 2: NP_001018177.1
• Gene: capn2b / 563053
• ZFINID: ZDB-GENE-050522-84
• Length: 696
• Species: Danio rerio
• Alignment Length: 727
• Identity: 306/727 - (42%)
• Similarity: 432/727 - (59%)
• Gaps: 67/727 - (9%)

  Fly   230 GRKATSARQNSVSKG-------------DFQSLRDSCLANGTMFEDPDFPATNASLMY-----SR 276
            |..:|.|::.:::.|             ||::||..|.|.||:|.||.|||...||.:     :.
Zfish     3 GIASTVAKKRALAAGFGTNANATKYLNQDFEALRSECRARGTLFSDPTFPAAPESLGFKELGPNS 67

  Fly   277 RPDRYYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQ 341
            ...|...|.|||::...|:|.|.|.::.|:.||.|||||||||.|:|||:..:..||:|..|:|.
Zfish    68 SKTRGLVWKRPGELCQKPRFIVGGATKTDICQGALGDCWLLAAIASLTQNEDVLARVVPNGQEFD 132

  Fly   342 ENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKG 406
            ..|||||||:|||:|:||:||||||||..:|||::::|.||||||||||||||||::|.||||.|
Zfish   133 GTYAGIFHFQFWQFGEWVDVVIDDRLPVKDGELLFVYSAEKNEFWSALLEKAYAKVNGCYEALSG 197

  Fly   407 GTTCEAMEDFTGGVTEWYDIKEAPPNLFSIMMKAAERGSMMGCSLE----PDPHVLEAETPQGLI 467
            |:|.|..||||||:.|.|:|::||.|||.|:.||.|.|:::|||::    .|.   ||.|.|.|:
Zfish   198 GSTSEGFEDFTGGIAESYEIRKAPTNLFQIIQKALEAGALLGCSIDITSAADS---EAITRQKLV 259

  Fly   468 RGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFI-----PEHTKEE 527
            :|||||:|..  .:::...|:.||  :||||||| ..||:|.|||:||||..:     |:.:.|
Zfish   260 KGHAYSLTGA--TEVNYRGRKEKL--VRMRNPWG-QVEWTGAWSDNSPEWNSVAASERPDASAE- 318

  Fly   528 IGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGC 592
                   ||||||:|.:||.::.|:|||.|:||::|:|    |.:.|.::..:|.|..|.|||||
Zfish   319 -------DGEFWMAFSEFLTNYSRIEICTLTPDAITDD----SVKHWAVTNHDGTWRRGSTAGGC 372

  Fly   593 RNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLT---- 653
            ||...|||.|||:::.|::.:.||.:..|:.:|.|:|||||..|..|.|..|||||||.|.
Zfish   373 RNNPYTFWMNPQFVVKLDEENVEDKEKGCSFVVGLIQKNRRRLRKTGEDMNTIGFAIYELPQEFH 437

  Fly   654 -DRDMQVKPQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFS 717
             .|::.:..   |||......|||..|||.||||.||.||||.||||||||:.|::|:|.:||||
Zfish   438 GQREVHLDK---NFFLTHGQKARSETFINLREVCTRFHLPPGEYLIVPSTFEANKDGDFCLRVFS 499

  Fly   718 ETRNNMEENDDEVGFGETDDRIAPSLPPPTPKEEDDPQRIALRRLFDSVAGSDEEVDWQELKRIL 782
            |:::..:..||.|.....|:.::           ||......|.:|..:||.|.|:...||..|.
Zfish   500 ESQSETQHCDDPVDAELPDETVS-----------DDQVDSKFRGMFVKLAGPDMEISPLELMTIF 553

  Fly   783 DHSM-RDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSI 846
            :..: :...:.|..|:.|..|.||.::|...:|:||..||..|...:.::..:||..|...:|:|
Zfish   554 NKIIAKRTDIKSSTFTLDTCRVMVNLMDDSGNGKLGLGEFATLWKKVQRYLEIFKHNDLDSSGTI 618

  Fly   847 DGFHLRGALNSAGYHLNNRLLNALAHRYGSREGQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTA 911
            ....||.||..||:.|||.|...:..||..::..:.||:|:.|.:::......|:..|...|...
Zfish   619 STPELRMALKEAGFCLNNTLFQLMVARYAEKDMTLLFDNFVSCLMRLEMMFRAFKRLDPHKSGFI 683

  Fly   912 FFNLDDWLERTI 923
            ..|.:.||..|:
Zfish   684 ELNFNQWLNLTM 695

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CalpB	NP_524016.4	PHA03247	<40..207	CDD:223021
		Peptidase_C2	260..556	CDD:459889	156/309 (50%)
		Calpain_III	579..716	CDD:460050	70/141 (50%)
		EFh_PEF_CalpA_B	758..925	CDD:320071	50/167 (30%)
		EF-hand motif	758..786	CDD:320071	9/27 (33%)
		EF-hand motif	799..829	CDD:320071	11/29 (38%)
		EF-hand motif	830..860	CDD:320071	10/29 (34%)
		EF-hand motif	866..894	CDD:320071	7/27 (26%)
		EF-hand motif	895..925	CDD:320071	7/29 (24%)
capn2b	NP_001018177.1	Peptidase_C2	46..339	CDD:459889	155/308 (50%)
		Calpain_III	359..498	CDD:460050	70/141 (50%)
		EFh_PEF	530..696	CDD:355382	50/166 (30%)
		EF-hand motif	530..557	CDD:320054	9/26 (35%)
		EF-hand motif	572..601	CDD:320054	10/28 (36%)
		EF-hand motif	602..631	CDD:320054	9/28 (32%)
		EF-hand motif	638..666	CDD:320054	7/27 (26%)
		EF-hand motif	668..696	CDD:320054	7/28 (25%)