DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpB and capn3a

DIOPT Version :9

Sequence 1:	NP_001246706.1	Gene:	CalpB / 39165	FlyBaseID:	FBgn0025866	Length:	925	Species:	Drosophila melanogaster
Sequence 2:	XP_021322825.1	Gene:	capn3a / 447832	ZFINID:	ZDB-GENE-040912-97	Length:	736	Species:	Danio rerio

Alignment Length:	737	Identity:	325/737 - (44%)
Similarity:	459/737 - (62%)	Gaps:	72/737 - (9%)

- Green bases have known domain annotations that are detailed below.

  Fly   238 QNSVSK------GDFQSLRDSCLANGTMFEDPDFPATNASLMYSRRPDRYYE-----WLRPGDIA 291
            :.|:||      .|:..|:...|...|:|||..||||..||.|.....:..:     |.||.:|.
Zfish    23 EGSLSKPIRFSGQDYTLLKQEYLQKKTLFEDETFPATVDSLGYKELGHKSNKVKNIVWKRPKEIC 87

  Fly   292 DDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYG 356
            |:|||.|.|.||.|:.||:|||||||||.|.||.:..|.:||||.:|.|.|.|||||||:||:||
Zfish    88 DNPQFIVGGASRTDICQGDLGDCWLLAAIACLTLNDKLLYRVIPQEQSFSEQYAGIFHFQFWRYG 152

  Fly   357 KWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVT 421
            .||:||:|||:||:|.:|::..|.|:|||||||||||||||||||||||||.|.|.|||||||||
Zfish   153 DWVDVVVDDRIPTFNNQLVFTKSAERNEFWSALLEKAYAKLHGSYEALKGGNTAEGMEDFTGGVT 217

  Fly   422 EWYDIKEAPPNLFSIMMKAAERGSMMGCSLEP-DPHVLEAETPQGLIRGHAYSITKV--CLMDIS 483
            |:|::||||..|:.||.||.||||:||||::. .|...|..|..||::|||||:|.|  |.   .
Zfish   218 EFYEMKEAPKELYKIMQKALERGSLMGCSIDSLVPARFETRTATGLVKGHAYSVTAVEECK---Q 279

  Fly   484 TPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNH 548
            :..::.::.::|:||||| ..||:|||||:|.||..:.:..||::......||||||||:||..:
Zfish   280 SQQKESRVRLVRLRNPWG-QVEWNGPWSDNSKEWESLSKAEKEKLQQQNAEDGEFWMSFEDFKKN 343

  Fly   549 FDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISL--ED 611
            :.::|||||:||:|.:|:.|    ||.:|:.||.|..|.:||||||:.:|||.||||.:.|  ||
Zfish   344 YTKIEICNLTPDALEDDKLH----KWTVSVNEGRWVRGCSAGGCRNYPDTFWTNPQYRLRLLEED 404

  Fly   612 PDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGL--NFFKYRASVA 674
            .|.|||:..||.:|||||||||.:|.:|.:.|||||:||.: .::|....|.:  .||....:.|
Zfish   405 DDPEDDEVACTFVVALMQKNRRKERKLGANLLTIGFSIYEV-PKEMHGNKQHMQKEFFMSTTAKA 468

  Fly   675 RSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEVGFGETDDRI 739
            ||..:||.|||..||:|.||.|:|:||:::|::|||||:|||||.||..|         |.::||
Zfish   469 RSRAYINLREVTQRFRLSPGEYVIIPSSYEPHQEGEFILRVFSEKRNTSE---------EIENRI 524

  Fly   740 APSLPPPTP-----KEEDDPQRIALRRLFDSVA------------GSDEEVDWQELKRILDHSM- 786
            ....|.|:|     :.|:|.|.::   :|..:|            ||:.||...|||.:|:..:
Zfish   525 EADHPVPSPASTGEESEEDQQFLS---IFQHIAGDVSTGEAVSAWGSNMEVSANELKDVLNKVVS 586

  Fly   787 --RDVMVGSDGFSKDAVRSMVAMLD---KDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSI 846
              :|  :.::.||::..|||:|::|   .|.:|||..:||..|...|.:|:.:||.||.....:|
Zfish   587 KHKD--IHTESFSRECCRSMIALMDVSFMDGTGRLNLQEFRQLWNKIKQWQEIFKRYDFDHNDTI 649

  Fly   847 DGFHLRGALNSA--------GYHLNNRLLNALAHRYGSREGQIPFDDFLMCAIKVRTFIEMFRER 903
            ..:.:|.|:|.|        |:.|||:|.:.:..||.:....:.|:.|:.|.:::......|:..
Zfish   650 SSYEMRNAINDAVTCLVCHSGFRLNNQLYDIITMRYANESMNVDFESFISCLVRLEGMFRAFQAF 714

  Fly   904 DTDNSDTAFFNLDDWLERTIYS 925
            |.....:...::.:||:.|:|:
Zfish   715 DQCGDGSIRLSVLEWLQLTMYA 736

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpB	NP_001246706.1	PD-C2-AF1	33..225	CDD:286403
		Drf_FH1	37..189	CDD:283903
		Peptidase_C2	260..556	CDD:279042	167/303 (55%)
		Calpain_III	574..721	CDD:279416	78/150 (52%)
		EF-hand_7	759..825	CDD:290234	24/83 (29%)
		EFh	761..826	CDD:298682	25/82 (30%)
		FRQ1	827..>908	CDD:227455	23/88 (26%)
capn3a	XP_021322825.1	Peptidase_C2	51..351	CDD:306994	167/303 (55%)
		Calpain_III	372..515	CDD:307285	75/143 (52%)
		EFh_PEF	545..736	CDD:330173	52/195 (27%)
		EF-hand motif	545..585	CDD:320054	11/42 (26%)
		EF-hand motif	600..632	CDD:320054	12/31 (39%)
		EF-hand motif	633..670	CDD:320054	10/36 (28%)
		EF-hand motif	677..705	CDD:320054	6/27 (22%)
		EF-hand motif	707..735	CDD:320054	5/27 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	358	1.000	Domainoid score	I921
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	656	1.000	Inparanoid score	I755
OMA	1	1.010	-	-		QHG48339
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm6388
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	LDO	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.980