Protein Alignment CalpB and capn9

DIOPT Version :10

• Sequence 1: NP_524016.4
• Gene: CalpB / 39165
• FlyBaseID: FBgn0025866
• Length: 925
• Species: Drosophila melanogaster
• Sequence 2: NP_001003501.1
• Gene: capn9 / 445107
• ZFINID: ZDB-GENE-010724-2
• Length: 688
• Species: Danio rerio
• Alignment Length: 711
• Identity: 331/711 - (46%)
• Similarity: 455/711 - (63%)
• Gaps: 32/711 - (4%)

  Fly   217 SVKVPENQNMFWMGRKATSARQNSVSKG-DFQSLRDSCLANGTMFEDPDFPATNASLMYSRRPDR 280
            ||.:|.:...       |:..:::.:.| .::.||..||..|.:||||||||.:.||.||:|...
Zfish     4 SVTLPGSSGQ-------TAVVESTQADGKTYEQLRRECLQKGKLFEDPDFPAVDGSLFYSQRVPV 61

  Fly   281 YYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYA 345
            .:||.|||:|..:|||...|.:|.|:.||:|||||||||.|:||.......||:|.||.|...||
Zfish    62 NFEWKRPGEICKEPQFITGGATRTDICQGQLGDCWLLAAIASLTLKEETLNRVVPNDQTFDRGYA 126

  Fly   346 GIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTC 410
            |||||:|||:.||::||:|||||....:|:::||.:.|||||||||||||||:|||||||||:|.
Zfish   127 GIFHFQFWQHNKWMDVVVDDRLPCVRDKLVFLHSADNNEFWSALLEKAYAKLNGSYEALKGGSTM 191

  Fly   411 EAMEDFTGGVTEWYDIKEAPPNLFSIMMKAAERGSMMGCSLE-PDPHVLEAETPQGLIRGHAYSI 474
            ||||||||||.|.|:.|..|.|||.|:.||.|||||:|||:: ......||:|..||::|||||:
Zfish   192 EAMEDFTGGVGEMYETKNGPSNLFLILKKAIERGSMLGCSIDITSSAESEAQTSTGLVKGHAYSV 256

  Fly   475 TKVCLMDISTPNRQG-KLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEF 538
            |     .:...|.:| |:.:||:||||| ..||:|||||:|.||..|....|:.:..|...||||
Zfish   257 T-----GVEEVNYRGAKVQLIRVRNPWG-QVEWNGPWSDNSREWTVIDSSEKKRLLQNSLDDGEF 315

  Fly   539 WMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNP 603
            ||.|.||.:::|:|||||||||:|::|..    ::|.:::|||.|..|.|||||||:::|||.||
Zfish   316 WMEFGDFKSNYDKVEICNLSPDALSDDVS----KQWAVNLFEGSWIRGSTAGGCRNYIDTFWTNP 376

  Fly   604 QYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGLNFFK 668
            |:.:.||:||  |.|..|:.||||||||||..|..|:|..|||||||...|   ....||.:||:
Zfish   377 QFKLHLENPD--DGDKLCSVIVALMQKNRRRLRKEGLDLETIGFAIYEAPD---DQDHQGKDFFR 436

  Fly   669 YRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEVGFG 733
            |..|.|||..:||.|||..||:||||:||:||:||.|::|.:|:||:|||.:....|..:::
Zfish   437 YNPSKARSRTYINVREVSERFRLPPGNYLLVPTTFQPHKEADFLIRLFSEKKAGAIEVGNKI--- 498

  Fly   734 ETDDRIAPSLPPPTPKEEDDPQRIALRRLFDSVAGSDEEVDWQELKRILDHSM-RDVMVGSDGFS 797
            |.|   .|..|.|.|.||:..:...||:||:.|||.|..:..:||:.:|::.: |...|..||.|
Zfish   499 EAD---LPEPPKPNPPEEETDEEKGLRKLFEQVAGPDNVISARELQHVLNNVLGRRKEVKFDGLS 560

  Fly   798 KDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHL 862
            .:...|::.::|.|.||.:.|.||:.....:.||..:|..||..|:|::..:.||.|||:||..|
Zfish   561 LNTCISIINLMDVDGSGMMEFSEFKVFWDKLKKWIMLFLSYDVDRSGTMSSYELRSALNAAGMKL 625

  Fly   863 NNRLLNALAHRYGSREGQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTI 923
            |||:|..|..|:...:.:|.|||:|.|.:::.....:|:..|.........|:..:|..|:
Zfish   626 NNRILQLLGLRFADEKLEIDFDDYLTCIVRLENMFRIFQALDAQKKGEVSLNMHQFLLLTM 686

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CalpB	NP_524016.4	PHA03247	<40..207	CDD:223021
		Peptidase_C2	260..556	CDD:459889	167/297 (56%)
		Calpain_III	579..716	CDD:460050	77/136 (57%)
		EFh_PEF_CalpA_B	758..925	CDD:320071	56/167 (34%)
		EF-hand motif	758..786	CDD:320071	11/27 (41%)
		EF-hand motif	799..829	CDD:320071	8/29 (28%)
		EF-hand motif	830..860	CDD:320071	13/29 (45%)
		EF-hand motif	866..894	CDD:320071	9/27 (33%)
		EF-hand motif	895..925	CDD:320071	5/29 (17%)
capn9	NP_001003501.1	Peptidase_C2	41..333	CDD:459889	167/297 (56%)
		Calpain_III	345..494	CDD:238132	81/153 (53%)
		EFh_PEF_CAPN9	520..688	CDD:320067	56/167 (34%)
		EF-hand motif	520..548	CDD:320067	11/27 (41%)
		EF-hand motif	563..592	CDD:320067	8/28 (29%)
		EF-hand motif	593..623	CDD:320067	13/29 (45%)
		EF-hand motif	629..657	CDD:320067	9/27 (33%)
		EF-hand motif	659..688	CDD:320067	5/28 (18%)