DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpB and CAPN8

DIOPT Version :9

Sequence 1:	NP_001246706.1	Gene:	CalpB / 39165	FlyBaseID:	FBgn0025866	Length:	925	Species:	Drosophila melanogaster
Sequence 2:	NP_001137434.1	Gene:	CAPN8 / 388743	HGNCID:	1485	Length:	703	Species:	Homo sapiens

Alignment Length:	734	Identity:	319/734 - (43%)
Similarity:	448/734 - (61%)	Gaps:	61/734 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly   207 SVGVAELSFTSVKVPENQN-MFWMGRKATSARQNSVSKGDFQSLRDSCLANGTMFEDPDFPATNA 270
            :.||:.....:..:..||| :.::|:             ||::||..||.:|.:|:||:|||..:
Human     5 AAGVSRQRAATQGLGSNQNALKYLGQ-------------DFKTLRQQCLDSGVLFKDPEFPACPS 56

  Fly   271 SLMY-----SRRPDRYYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLF 330
            :|.|     .....:...|.||.::...|||.|.|.:|.|:.||.|||||||||.|:||.:..|.
Human    57 ALGYKDLGPGSPQTQGIIWKRPTELCPSPQFIVGGATRTDICQGGLGDCWLLAAIASLTLNEELL 121

  Fly   331 FRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYA 395
            :||:|.||||||||||||||:|||||:||||||||||||.||:|:::||.:.|||||||||||||
Human   122 YRVVPRDQDFQENYAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEQGNEFWSALLEKAYA 186

  Fly   396 KLHGSYEALKGGTTCEAMEDFTGGVTEWYDIKEAPPNLFSIMMKAAERGSMMGCSLEPDPHV-LE 459
            ||:|.||||.||:|.|..||||||::|:||:|:.|.||:.|:.||...||::|||::..... .|
Human   187 KLNGCYEALAGGSTVEGFEDFTGGISEFYDLKKPPANLYQIIRKALCAGSLLGCSIDVSSAAEAE 251

  Fly   460 AETPQGLIRGHAYSITKVCLMDISTPNRQGKLP--MIRMRNPWGNDAEWSGPWSDSSPEWRFIPE 522
            |.|.|.|::.||||:|     .:...|.||. |  :||:||||| :.||||.|||.:|||..|..
Human   252 AITSQKLVKSHAYSVT-----GVEEVNFQGH-PEKLIRLRNPWG-EVEWSGAWSDDAPEWNHIDP 309

  Fly   523 HTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGV 587
            ..|||:....: |||||||..||:..|.|:||||||||||:.::.|    ||.:.:|.|.||.|.
Human   310 RRKEELDKKVE-DGEFWMSLSDFVRQFSRLEICNLSPDSLSSEEVH----KWNLVLFNGHWTRGS 369

  Fly   588 TAGGCRNFLETFWHNPQYIISLE--DPDDEDDDGK--CTAIVALMQKNRRSKRNVGIDCLTIGFA 648
            |||||:|:..|:|.|||:.|.|:  |.|.|:..|:  ||.::.|||||||.::.:|...|:||:|
Human   370 TAGGCQNYPATYWTNPQFKIRLDEVDEDQEESIGEPCCTVLLGLMQKNRRWRKRIGQGMLSIGYA 434

  Fly   649 IYHL-------TDRDMQVKPQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPN 706
            :|.:       ||..:     |.:||......||:..::|.|||..|.:||||.||:|||||:|.
Human   435 VYQVPKELESHTDAHL-----GRDFFLAYQPSARTSTYVNLREVSGRARLPPGEYLVVPSTFEPF 494

  Fly   707 EEGEFIIRVFSETRNNMEENDDEVGFGETDDRIAPSLPPPTPKEEDDPQRIALRRLFDSVAGSDE 771
            ::|||.:|||||.:....|..|.|...       |..|.|:..:::|.|   .||||:.:||.|.
Human   495 KDGEFCLRVFSEKKAQALEIGDVVAGN-------PYEPHPSEVDQEDDQ---FRRLFEKLAGKDS 549

  Fly   772 EVDWQELKRILDHSM-RDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVF 835
            |:....||.:|:.:. :...:..|||:.:..|.|:::||.:.:|.||..||:.|...|.|:..::
Human   550 EITANALKILLNEAFSKRTDIKFDGFNINTCREMISLLDSNGTGTLGAVEFKTLWLKIQKYLEIY 614

  Fly   836 KLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSREGQIPFDDFLMCAIKVRTFIEMF 900
            ...|...:|:||...:|.||..||:.||:::...:|.||...:..|.||.|:.|.|::.|..::|
Human   615 WETDYNHSGTIDAHEMRTALRKAGFTLNSQVQQTIALRYACSKLGINFDSFVACMIRLETLFKLF 679

  Fly   901 RERDTDNSDTAFFNLDDWL 919
            ...|.|.......:|.:||
Human   680 SLLDEDKDGMVQLSLAEWL 698

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpB	NP_001246706.1	PD-C2-AF1	33..225	CDD:286403	3/17 (18%)
		Drf_FH1	37..189	CDD:283903
		Peptidase_C2	260..556	CDD:279042	165/303 (54%)
		Calpain_III	574..721	CDD:279416	70/157 (45%)
		EF-hand_7	759..825	CDD:290234	23/66 (35%)
		EFh	761..826	CDD:298682	23/65 (35%)
		FRQ1	827..>908	CDD:227455	26/80 (33%)
CAPN8	NP_001137434.1	Peptidase_C2	46..342	CDD:279042	165/303 (54%)
		Calpain_III	356..509	CDD:279416	70/157 (45%)
		Domain III	356..379		12/22 (55%)
		EFh	580..635	CDD:238008	18/54 (33%)
		PTZ00183	618..>692	CDD:185503	24/73 (33%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165146560
Domainoid	1	1.000	356	1.000	Domainoid score	I986
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	643	1.000	Inparanoid score	I809
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG48339
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm8590
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	13	12.810