DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpB and Capn3

DIOPT Version :9

Sequence 1:	NP_001246706.1	Gene:	CalpB / 39165	FlyBaseID:	FBgn0025866	Length:	925	Species:	Drosophila melanogaster
Sequence 2:	NP_058813.2	Gene:	Capn3 / 29155	RGDID:	2269	Length:	821	Species:	Rattus norvegicus

Alignment Length:	879	Identity:	346/879 - (39%)
Similarity:	483/879 - (54%)	Gaps:	157/879 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly   146 LPYPAAP-LAPYPSAMPGLPGMPMPY-APMPTSPAPQHNIGFPALPYPTAPPPESAPTQEEEPSV 208
            :|...:| :||...|.|..|| |:|: |...|:.|   ..|.|...|       ||......|.:
  Rat     1 MPTVISPTVAPRTGAEPRSPG-PVPHPAQGKTTEA---GGGHPGGIY-------SAIISRNFPII 54

  Fly   209 GVAELSFTSVKVPENQNMFWMGRKATSARQNSVSKGDFQSLRDSCLANGTMFEDPDFPATNASLM 273
            ||.|.:                               |:.|...||....::.||:||....||.
  Rat    55 GVKEKT-------------------------------FEQLHKKCLEKKVLYLDPEFPPDETSLF 88

  Fly   274 YSRRPDRYYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQ 338
            ||::....:.|.||.:|.::|:|.:.|.:|.|:.||:|||||.|||.|.||.:..|.|||||.||
  Rat    89 YSQKFPIQFVWKRPPEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQ 153

  Fly   339 DFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEA 403
            .|.|||||||||:||:||.||:|||||.|||||.:|::..|..:|||||||||||||||||||||
  Rat   154 SFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEA 218

  Fly   404 LKGGTTCEAMEDFTGGVTEWYDIKEAPPNLFSIMMKAAERGSMMGCSLEPDPHVLEAETPQ---- 464
            ||||.|.||||||||||||:::||:||.:::.||.||.||||:||||::...::....:|.
  Rat   219 LKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGTSPSGLNM 283

  Fly   465 ---------------------------------------------GLIRGHAYSIT--KVCLMDI 482
                                                         ||::|||||:|  :..|.
  Rat   284 GELIARMVRNMDNSLLRDSDLDPRASDDRPSRTIVPVQYETRMACGLVKGHAYSVTGLEEALF-- 346

  Fly   483 STPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLN 547
                :..|:.::|:||||| ..||:|.|||...:|.|:.:..|..:......|||||||:.||:.
  Rat   347 ----KGEKVKLVRLRNPWG-QVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVY 406

  Fly   548 HFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDP 612
            ||.::|||||:.|:|..|:..:    |.:|:.||.|..|.:|||||||.:|||.||||.:.|.:.
  Rat   407 HFTKLEICNLTADALESDKLQT----WTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEE 467

  Fly   613 DDEDDDGK--CTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGL--NFFKYRASV 673
            ||:.||.:  |:.:|||||||||..|.:|.:..|||||||.: .::|....|.|  :||.|.||.
  Rat   468 DDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEV-PKEMHGNKQHLQKDFFLYNASK 531

  Fly   674 ARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEE------------- 725
            |||..:||.|||..||:|||..|:|||||::|::|||||:|||||.||..||
  Rat   532 ARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAENTISVDRPVKK 596

  Fly   726 --------------NDDEVGFG----ETDDRIAP----SLPPPTP----KEEDDPQRIALRRLFD 764
                          ::.|:|..    |..|:..|    ..|.|.|    :|.::.|:  .|.:|.
  Rat   597 KKNKPIIFVSDRANSNKELGVDQEAEEGKDKTGPDKQGESPQPRPGHTDQESEEQQQ--FRNIFR 659

  Fly   765 SVAGSDEEVDWQELKRILD---HSMRDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLT 826
            .:||.|.|:...|||.:|:   :..:|:.  :.||:.::.|||:|::|.|.||||..:||..|..
  Rat   660 QIAGDDMEICADELKNVLNTVVNKHKDLK--TQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWK 722

  Fly   827 DIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSREGQIPFDDFLMCAI 891
            .|..|:.:||.|||..:|:|:.:.:|.|:|.||:|||::|.:.:..||..:...|.||.|:.|.:
  Rat   723 KIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFV 787

  Fly   892 KVRTFIEMFRERDTDNSDTAFFNLDDWLERTIYS 925
            ::......|...|.|.......|:.:||:.|:|:
  Rat   788 RLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA 821

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpB	NP_001246706.1	PD-C2-AF1	33..225	CDD:286403	22/80 (28%)
		Drf_FH1	37..189	CDD:283903	15/44 (34%)
		Peptidase_C2	260..556	CDD:279042	159/346 (46%)
		Calpain_III	574..721	CDD:279416	82/150 (55%)
		EF-hand_7	759..825	CDD:290234	25/68 (37%)
		EFh	761..826	CDD:298682	25/67 (37%)
		FRQ1	827..>908	CDD:227455	28/80 (35%)
Capn3	NP_058813.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..37		13/39 (33%)
		Peptidase_C2	77..415	CDD:395523	159/344 (46%)
		Domain III	418..586		89/172 (52%)
		Calpain_III	436..574	CDD:395848	75/138 (54%)
		Calpain_u2	583..653	CDD:406940	10/69 (14%)
		Linker	587..649		9/61 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	603..651		9/47 (19%)
		Domain IV	650..820		59/173 (34%)
		EFh_PEF_CAPN3	653..821	CDD:320065	58/171 (34%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	362	1.000	Domainoid score	I918
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	641	1.000	Inparanoid score	I782
OMA	1	1.010	-	-		QHG48339
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm9065
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	LDO	PTHR10183
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.880