DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpB and Capn8

DIOPT Version :9

Sequence 1:	NP_001246706.1	Gene:	CalpB / 39165	FlyBaseID:	FBgn0025866	Length:	925	Species:	Drosophila melanogaster
Sequence 2:	NP_579843.2	Gene:	Capn8 / 170808	RGDID:	620085	Length:	703	Species:	Rattus norvegicus

Alignment Length:	716	Identity:	319/716 - (44%)
Similarity:	452/716 - (63%)	Gaps:	58/716 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly   236 ARQNSVSKG-------------DFQSLRDSCLANGTMFEDPDFPATNASLMYS----RRPD-RYY 282
            ::|.:|::|             ||::||..||.:|.:|:||:|||..::|.|.    ..|| :..
  Rat     9 SKQRAVAEGLGSNQNAVKYLGQDFETLRKQCLNSGVLFKDPEFPACPSALGYKDLGPGSPDTQGI 73

  Fly   283 EWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGI 347
            .|.||.::..:|||.|.|.:|.|::||.|||||||||.|:||.:..|.:||:|.||.||::||||
  Rat    74 VWKRPTELCPNPQFIVGGATRTDIRQGGLGDCWLLAAIASLTLNEKLLYRVLPRDQSFQKDYAGI 138

  Fly   348 FHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEA 412
            |||:|||||:||||||||||||.||:|:::||.|.|||||||||||||||:||||||.||:|.|.
  Rat   139 FHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEALVGGSTIEG 203

  Fly   413 MEDFTGGVTEWYDIKEAPPNLFSIMMKAAERGSMMGCSLEPDPHV-LEAETPQGLIRGHAYSITK 476
            .||||||::|:||:|:.|.||:.|:.||..:||::|||::..... .||.|.|.|::|||||:|.
  Rat   204 FEDFTGGISEFYDLKKPPENLYYIIQKALRKGSLLGCSIDVSTAAEAEATTRQKLVKGHAYSVTG 268

  Fly   477 VCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMS 541
            |  .:::...|..||  ||:||||| :.||||.|||::|||.:|....|||:....: |||||||
  Rat   269 V--EEVNFHGRPEKL--IRLRNPWG-EVEWSGAWSDNAPEWNYIDPRRKEELDKKAE-DGEFWMS 327

  Fly   542 FQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYI 606
            |.|||..:.|:||||||||||:.::.|    ||.:.:|.|.||.|.|||||.|:..|:|.|||:.
  Rat   328 FSDFLKQYSRLEICNLSPDSLSSEEIH----KWNLVLFNGRWTRGSTAGGCLNYPGTYWTNPQFK 388

  Fly   607 ISLEDPDDEDDDGK----CTAIVALMQKNRRSKRNVGIDCLTIGFAIYHL-------TDRDMQVK 660
            |.|::.|::.::|.    ||.::.|||||||.::.:|...|:||:|:|.:       ||..:
  Rat   389 IHLDEVDEDQEEGTSEPCCTVLLGLMQKNRRRQKRIGQGMLSIGYAVYQIPKELESHTDAHL--- 450

  Fly   661 PQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEE 725
              |.:||..|.....|..::|.|||.:|.:||||.||:|||||:|.::|:|.:|||||.:....|
  Rat   451 --GRDFFLGRQPSTCSSTYMNLREVSSRVRLPPGQYLVVPSTFEPFKDGDFCLRVFSEKKAKALE 513

  Fly   726 NDDEVGFGETDDRIAPSLPPPTPKEEDDPQRIALRRLFDSVAGSDEEVDWQELKRILDH--SMRD 788
            ..|.|. |.      |..|.|...:|:|..   :|.||:...|.|.|:...:|||:|:.  |.|.
  Rat   514 IGDTVS-GH------PHEPHPRDMDEEDEH---VRSLFEEFVGKDSEISANQLKRVLNEVLSKRT 568

  Fly   789 VMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRG 853
            .| ..|||:.:..|.|:::||.|.:|.||..||:.|...|..:..:|:..|....|:|:...:|.
  Rat   569 DM-KFDGFNINTCREMISLLDSDGTGSLGPMEFKTLWLKIRTYLEIFQEMDHNHVGTIEAHEMRT 632

  Fly   854 ALNSAGYHLNNRLLNALAHRYGSREGQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDW 918
            ||..||:.|||::...:|.||...:..:.|:.|:.|.|::.|..::||..|.|.:.....:|.:|
  Rat   633 ALKKAGFTLNNQVQQTIAMRYACSKLGVDFNGFVACMIRLETLFKLFRLLDKDQNGIVQLSLAEW 697

  Fly   919 L 919
            |
  Rat   698 L 698

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpB	NP_001246706.1	PD-C2-AF1	33..225	CDD:286403
		Drf_FH1	37..189	CDD:283903
		Peptidase_C2	260..556	CDD:279042	168/301 (56%)
		Calpain_III	574..721	CDD:279416	67/157 (43%)
		EF-hand_7	759..825	CDD:290234	26/67 (39%)
		EFh	761..826	CDD:298682	26/66 (39%)
		FRQ1	827..>908	CDD:227455	25/80 (31%)
Capn8	NP_579843.2	Peptidase_C2	46..342	CDD:279042	168/301 (56%)
		Domain III	355..512		68/161 (42%)
		Calpain_III	356..509	CDD:279416	67/157 (43%)
		Linker. /evidence=ECO:0000250	513..531		7/24 (29%)
		Domain IV. /evidence=ECO:0000250	532..703		57/171 (33%)
		EFh	580..635	CDD:238008	18/54 (33%)
		PTZ00183	612..>692	CDD:185503	24/79 (30%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166340361
Domainoid	1	1.000	362	1.000	Domainoid score	I918
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	641	1.000	Inparanoid score	I782
OMA	1	1.010	-	-		QHG48339
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm9065
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	14	13.810