DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpB and Capn2

DIOPT Version :9

Sequence 1:	NP_001246706.1	Gene:	CalpB / 39165	FlyBaseID:	FBgn0025866	Length:	925	Species:	Drosophila melanogaster
Sequence 2:	NP_033924.2	Gene:	Capn2 / 12334	MGIID:	88264	Length:	700	Species:	Mus musculus

Alignment Length:	703	Identity:	325/703 - (46%)
Similarity:	450/703 - (64%)	Gaps:	58/703 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly   245 DFQSLRDSCLANGTMFEDPDFPATNASLMYSR-----RPDRYYEWLRPGDIADDPQFFVEGYSRF 304
            |:::||:.||..|.:|:||.|||..:||.|..     ...|..||.||.:|..||||.:.|.:|.
Mouse    31 DYETLRNECLEAGALFQDPSFPALPSSLGYKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRT 95

  Fly   305 DVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPT 369
            |:.||.|||||||||.|:||.:..:..||:||||.|||||||||||:|||||:|||||:||||||
Mouse    96 DICQGALGDCWLLAAIASLTLNEEILARVVPPDQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPT 160

  Fly   370 YNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDIKEAPPNLF 434
            .:|||:::||.|.:|||||||||||||::|.||||.||.|.|..||||||:.|||::::.|||||
Mouse   161 KDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELRKPPPNLF 225

  Fly   435 SIMMKAAERGSMMGCSLE----PDPHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGKL-PMI 494
            .|:.||.|:||::|||::    .|.   ||.|.|.|::|||||:|....::.|     |.| .:|
Mouse   226 KIIQKALEKGSLLGCSIDITSAADS---EAVTYQKLVKGHAYSVTGAEEVESS-----GSLQKLI 282

  Fly   495 RMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSP 559
            |:||||| ..||:|.|:|:.|.|..:....:..: .....||||||||.|||.|:.|:|||||:|
Mouse   283 RIRNPWG-QVEWTGKWNDNCPSWNTVDPEVRANL-TERQEDGEFWMSFSDFLRHYSRLEICNLTP 345

  Fly   560 DSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGK--CT 622
            |:||.|    |.:||:::..:|.|..|.|||||||:..|||.||||:|.||:.|::::||:  ||
Mouse   346 DTLTCD----SYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGERGCT 406

  Fly   623 AIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQ-----GLNFFKYRASVARSPHFINT 682
            .:|.|:||:||.:|.:|.|..||||.||.:.:   ::..|     |.|||....:..||..|||.
Mouse   407 FLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPE---ELTGQTNIHLGKNFFLTTRARERSDTFINL 468

  Fly   683 REVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEV--GFGETDDRIAPSLPP 745
            |||..|||||||.|::|||||:|:::|:|.||||||.:.:.:..|||:  ...|.|
Mouse   469 REVLNRFKLPPGEYVLVPSTFEPHKDGDFCIRVFSEKKADYQAVDDEIEANIEEID--------- 524

  Fly   746 PTPKEEDDPQRIALRRLFDSVAGSDEEVDWQELKRILDHSMRDVM-----VGSDGFSKDAVRSMV 805
              ..|||...  ..||||..:||.|.|:...||:.||    |.|:     :.|||||.:..:.||
Mouse   525 --ANEEDIDD--GFRRLFVQLAGEDAEISAFELQTIL----RRVLAKRQDIKSDGFSIETCKIMV 581

  Fly   806 AMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNAL 870
            .|||:|.||:||.:||..|.|.|.|::.:::..|..|:|:::.:.:|.||..||:.|..:|...:
Mouse   582 DMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKLPCQLHQVI 646

  Fly   871 AHRYGSREGQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTI 923
            ..|:...|..|.||:|:.|.:::.|..::|::.|.:|:.|...||..||..::
Mouse   647 VARFADDELIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIQLNLASWLSFSV 699

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpB	NP_001246706.1	PD-C2-AF1	33..225	CDD:286403
		Drf_FH1	37..189	CDD:283903
		Peptidase_C2	260..556	CDD:279042	164/305 (54%)
		Calpain_III	574..721	CDD:279416	76/153 (50%)
		EF-hand_7	759..825	CDD:290234	31/70 (44%)
		EFh	761..826	CDD:298682	31/69 (45%)
		FRQ1	827..>908	CDD:227455	22/80 (28%)
Capn2	NP_033924.2	Peptidase_C2	46..342	CDD:279042	164/305 (54%)
		Domain III	345..514		83/175 (47%)
		Calpain_III	356..507	CDD:279416	76/153 (50%)
		Linker	515..529		4/24 (17%)
		Domain IV	530..700		61/176 (35%)
		EFh	577..631	CDD:238008	20/53 (38%)
		FRQ1	604..>690	CDD:227455	24/85 (28%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	357	1.000	Domainoid score	I968
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	640	1.000	Inparanoid score	I814
Isobase	1	0.950	-	0	Normalized mean entropy	S2747
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm8819
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.820