Protein Alignment CalpB and Capn2

DIOPT Version :10

• Sequence 1: NP_524016.4
• Gene: CalpB / 39165
• FlyBaseID: FBgn0025866
• Length: 925
• Species: Drosophila melanogaster
• Sequence 2: NP_033924.2
• Gene: Capn2 / 12334
• MGIID: 88264
• Length: 700
• Species: Mus musculus
• Alignment Length: 703
• Identity: 325/703 - (46%)
• Similarity: 450/703 - (64%)
• Gaps: 58/703 - (8%)

  Fly   245 DFQSLRDSCLANGTMFEDPDFPATNASLMYSR-----RPDRYYEWLRPGDIADDPQFFVEGYSRF 304
            |:::||:.||..|.:|:||.|||..:||.|..     ...|..||.||.:|..||||.:.|.:|.
Mouse    31 DYETLRNECLEAGALFQDPSFPALPSSLGYKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRT 95

  Fly   305 DVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPT 369
            |:.||.|||||||||.|:||.:..:..||:||||.|||||||||||:|||||:|||||:||||||
Mouse    96 DICQGALGDCWLLAAIASLTLNEEILARVVPPDQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPT 160

  Fly   370 YNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDIKEAPPNLF 434
            .:|||:::||.|.:|||||||||||||::|.||||.||.|.|..||||||:.|||::::.|||||
Mouse   161 KDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELRKPPPNLF 225

  Fly   435 SIMMKAAERGSMMGCSLE----PDPHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGKL-PMI 494
            .|:.||.|:||::|||::    .|.   ||.|.|.|::|||||:|....::.|     |.| .:|
Mouse   226 KIIQKALEKGSLLGCSIDITSAADS---EAVTYQKLVKGHAYSVTGAEEVESS-----GSLQKLI 282

  Fly   495 RMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSP 559
            |:||||| ..||:|.|:|:.|.|..:....:..: .....||||||||.|||.|:.|:|||||:|
Mouse   283 RIRNPWG-QVEWTGKWNDNCPSWNTVDPEVRANL-TERQEDGEFWMSFSDFLRHYSRLEICNLTP 345

  Fly   560 DSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGK--CT 622
            |:||.|    |.:||:::..:|.|..|.|||||||:..|||.||||:|.||:.|::::||:  ||
Mouse   346 DTLTCD----SYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGERGCT 406

  Fly   623 AIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQ-----GLNFFKYRASVARSPHFINT 682
            .:|.|:||:||.:|.:|.|..||||.||.:.:   ::..|     |.|||....:..||..|||.
Mouse   407 FLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPE---ELTGQTNIHLGKNFFLTTRARERSDTFINL 468

  Fly   683 REVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEV--GFGETDDRIAPSLPP 745
            |||..|||||||.|::|||||:|:::|:|.||||||.:.:.:..|||:  ...|.|
Mouse   469 REVLNRFKLPPGEYVLVPSTFEPHKDGDFCIRVFSEKKADYQAVDDEIEANIEEID--------- 524

  Fly   746 PTPKEEDDPQRIALRRLFDSVAGSDEEVDWQELKRILDHSMRDVM-----VGSDGFSKDAVRSMV 805
              ..|||...  ..||||..:||.|.|:...||:.||    |.|:     :.|||||.:..:.||
Mouse   525 --ANEEDIDD--GFRRLFVQLAGEDAEISAFELQTIL----RRVLAKRQDIKSDGFSIETCKIMV 581

  Fly   806 AMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNAL 870
            .|||:|.||:||.:||..|.|.|.|::.:::..|..|:|:::.:.:|.||..||:.|..:|...:
Mouse   582 DMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKLPCQLHQVI 646

  Fly   871 AHRYGSREGQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTI 923
            ..|:...|..|.||:|:.|.:::.|..::|::.|.:|:.|...||..||..::
Mouse   647 VARFADDELIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIQLNLASWLSFSV 699

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CalpB	NP_524016.4	PHA03247	<40..207	CDD:223021
		Peptidase_C2	260..556	CDD:459889	164/305 (54%)
		Calpain_III	579..716	CDD:460050	71/143 (50%)
		EFh_PEF_CalpA_B	758..925	CDD:320071	61/171 (36%)
		EF-hand motif	758..786	CDD:320071	12/27 (44%)
		EF-hand motif	799..829	CDD:320071	14/29 (48%)
		EF-hand motif	830..860	CDD:320071	8/29 (28%)
		EF-hand motif	866..894	CDD:320071	8/27 (30%)
		EF-hand motif	895..925	CDD:320071	9/29 (31%)
Capn2	NP_033924.2	Peptidase_C2	46..342	CDD:459889	164/305 (54%)
		Domain III	345..514		83/175 (47%)
		Calpain_III	354..512	CDD:238132	77/160 (48%)
		Linker	515..529		4/24 (17%)
		Domain IV	530..700		61/176 (35%)
		EFh_PEF_CAPN2	533..700	CDD:320074	61/171 (36%)
		EF-hand motif	533..561	CDD:320074	13/31 (42%)
		EF-hand motif	576..605	CDD:320074	14/28 (50%)
		EF-hand motif	606..636	CDD:320074	8/29 (28%)
		EF-hand motif	642..670	CDD:320074	8/27 (30%)
		EF-hand motif	672..700	CDD:320074	8/28 (29%)