DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpB and capn3

DIOPT Version :9

Sequence 1:	NP_001246706.1	Gene:	CalpB / 39165	FlyBaseID:	FBgn0025866	Length:	925	Species:	Drosophila melanogaster
Sequence 2:	XP_002935381.2	Gene:	capn3 / 100497532	XenbaseID:	XB-GENE-5875739	Length:	805	Species:	Xenopus tropicalis

Alignment Length:	825	Identity:	329/825 - (39%)
Similarity:	471/825 - (57%)	Gaps:	115/825 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly   195 PPESAPTQEEEPSVGVAELSFTSVKVPENQNMFWMGRKATSARQNSVSKG---DFQSLRDSCLAN 256
            |.|..|.|.::...|.|.....|........::    .|..:|...:..|   .|:.||..||..
Frog     2 PSEILPEQAQKTESGSAVTGGASDGAAPGGGIY----SAIISRNLPIIGGREKTFEELRKKCLQR 62

  Fly   257 GTMFEDPDFPATNASLMYSRRPDRYYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAA 321
            ...|:|.:||...:||.||:.....:||.||.:|.::|||.:.|.:|.|:.||:|||||.|||.|
Frog    63 NFHFQDREFPPDESSLFYSQSFPIKFEWKRPPEICENPQFILGGANRTDICQGDLGDCWFLAAIA 127

  Fly   322 NLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFW 386
            .||.:..:.||||||||:|.:||||||||:||:||.||:|:|||.|||||.||::..|:::||||
Frog   128 CLTLNEKVLFRVIPPDQNFTDNYAGIFHFQFWRYGDWVDVIIDDYLPTYNNELVFTKSSQRNEFW 192

  Fly   387 SALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDIKEAPPNLFSIMMKAAERGSMMGCSL 451
            |||||||||||||||||||||.|.||||||||||||:|::||||.::::||.||.||||::|||:
Frog   193 SALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYELKEAPKDMYNIMKKAFERGSLIGCSI 257

  Fly   452 E------------------------------PD---------------PHVLEAETPQGLIRGHA 471
            :                              ||               |..||.....||::|||
Frog   258 DDKYGSSCGFAPPSNMEDAIMKMIESVGDTRPDTNGRGQPPKPVATLVPVQLETRMANGLVKGHA 322

  Fly   472 YSITKVCLMDISTPNRQGK-LPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRD 535
            ||:|     .:.....:|| :.::|:||||| ..||:|.|.|::.||..:.:..|..:......|
Frog   323 YSVT-----GVEETKLKGKPIKLVRLRNPWG-QVEWNGAWGDNAKEWTMVDKSEKTRLQHQVQED 381

  Fly   536 GEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFW 600
            ||||||:.||:.:|.:.|||||:||:|..|    |.:.|.:|:.||.|..|.:||||||:.:|:|
Frog   382 GEFWMSYDDFMKNFTKAEICNLTPDALDSD----SLQVWTVSVNEGRWVRGCSAGGCRNYPDTYW 442

  Fly   601 HNPQYIISL--EDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQG 663
            .||||.:.|  ||.|..|::..|:.:|||||||||..|..|.:..|||||||.: .::|....|.
Frog   443 TNPQYRLKLLEEDDDPADNEVVCSFVVALMQKNRRKDRKAGANLFTIGFAIYEV-PKEMHGNNQH 506

  Fly   664 L--NFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEEN 726
            |  :||.|.||.|:...:||.||||.||:|||..|:|:|||::|::|||||:|||||.||..||.
Frog   507 LQKDFFLYNASKAKCKSYINMREVCQRFRLPPSEYVIIPSTYEPHQEGEFILRVFSEKRNISEEV 571

  Fly   727 DDEVGF-----------------------------GETD-----------DRIAPSLPPPTPKEE 751
            ::::..                             |.||           |:.:.|....|.:|:
Frog   572 ENKIEAEKPIKKKKKPKPIIFVSDRSNSNKELNVDGATDEDQKKMDAEKKDKTSASTDTETEEEK 636

  Fly   752 DDPQRIALRRLFDSVAGSDEEVDWQELKRILDHSM-RDVMVGSDGFSKDAVRSMVAMLDKDRSGR 815
            .      .|.:|..:||.|.|:...||:.:|::.: :...:.|.||:.::.|||:|::|.|..||
Frog   637 Q------FRNIFQQIAGDDMEISADELQSVLNNVVNKHKTLKSSGFTLESCRSMIALMDTDGCGR 695

  Fly   816 LGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSREGQ 880
            |..:||..|...|.:|:.:|..:|:.::|:|..|.:|.|:|.||:||||:|.:.:..||.::...
Frog   696 LNLQEFYHLWQKIKQWQKIFLRFDSDQSGTISSFEMRNAINEAGFHLNNQLYDIITMRYANKRMD 760

  Fly   881 IPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTIYS 925
            :.||.|:.|.:::......|...|.|.......|:.:||:.|:|:
Frog   761 LDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA 805

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpB	NP_001246706.1	PD-C2-AF1	33..225	CDD:286403	7/29 (24%)
		Drf_FH1	37..189	CDD:283903
		Peptidase_C2	260..556	CDD:279042	162/341 (48%)
		Calpain_III	574..721	CDD:279416	78/150 (52%)
		EF-hand_7	759..825	CDD:290234	23/66 (35%)
		EFh	761..826	CDD:298682	23/65 (35%)
		FRQ1	827..>908	CDD:227455	26/80 (33%)
capn3	XP_002935381.2	Peptidase_C2	66..402	CDD:279042	162/341 (48%)
		Calpain_III	416..566	CDD:279416	78/150 (52%)
		Calpain_u2	570..637	CDD:293254	8/66 (12%)
		EFh	648..706	CDD:298682	20/57 (35%)
		EFh	681..735	CDD:238008	20/53 (38%)
		EFh	712..801	CDD:298682	27/88 (31%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	361	1.000	Domainoid score	I950
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	643	1.000	Inparanoid score	I801
OMA	1	1.010	-	-		QHG48339
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm9483
Panther	1	1.100	-	-	LDO	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	10	10.080