DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpB and capn3b

DIOPT Version :9

Sequence 1:	NP_001246706.1	Gene:	CalpB / 39165	FlyBaseID:	FBgn0025866	Length:	925	Species:	Drosophila melanogaster
Sequence 2:	NP_001230250.1	Gene:	capn3b / 100034405	ZFINID:	ZDB-GENE-041001-149	Length:	723	Species:	Danio rerio

Alignment Length:	747	Identity:	314/747 - (42%)
Similarity:	456/747 - (61%)	Gaps:	62/747 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly   212 ELSFTSVKVPENQ------------NMFWMGRKATSARQNSVSKG----DFQSLRDSCLANGTMF 260
            :.|.|||::.|..            |::    .|..:|..:|...    |||.|||..:....:|
Zfish     6 KFSETSVQLTEAAPQTVGSGFTAPGNIY----SAILSRNEAVKDARRLKDFQELRDKYVRKKVLF 66

  Fly   261 EDPDFPATNASLMYSRRPDRYYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQ 325
            |||.|||.::||.||.:.....||.||.:|..:|||.:.|.:|.|:.||:|||||||||.|.||.
Zfish    67 EDPLFPAQDSSLFYSEKFPLKLEWKRPMEICKNPQFILGGANRTDICQGDLGDCWLLAAIACLTL 131

  Fly   326 DSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALL 390
            :..:..||:|.||.|.|||||||||:||:||:||:||||||||||..:|::..|.:.|||||||:
Zfish   132 NDVILKRVVPHDQSFTENYAGIFHFQFWRYGEWVDVVIDDRLPTYKNKLVFTRSGQNNEFWSALM 196

  Fly   391 EKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDIKEAPPNLFSIMMKAAERGSMMGCSLEP-D 454
            |||||||||||||||||.:.||||||||||||:|:|.|||..|::||.||.:|||:|||:::. .
Zfish   197 EKAYAKLHGSYEALKGGNSLEAMEDFTGGVTEFYEITEAPKELYNIMRKALKRGSLMGCAIDTLV 261

  Fly   455 PHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGK--------LPMIRMRNPWGNDAEWSGPWS 511
            |.....:|..||:||||||:|.|         .|||        :.::|:|:|||    .:.|.:
Zfish   262 PTAQRTKTASGLVRGHAYSVTGV---------EQGKRKDGKDLMIRLVRVRDPWG----VAPPPA 313

  Fly   512 DSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEM 576
            ..|.:|..:.:..:::..|.....|||||.|::|..:|.::|||||:||:|.:||..    ||.:
Zfish   314 CKSNDWVELAKTEQDKQRLQPGEQGEFWMCFEEFQKNFTKLEICNLTPDTLEDDQMF----KWNV 374

  Fly   577 SMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGID 641
            ::.||.|..|.:|||||||.:|:|.|||:.:.|.:.|.::.  .||.:||||||.||.:|..|..
Zfish   375 TVHEGRWVKGCSAGGCRNFPDTYWTNPQFRLVLLEADAKEK--TCTVVVALMQKGRRVERCSGAT 437

  Fly   642 CLTIGFAIYHLTDRDMQVKPQ---GLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTF 703
            ...||||||.: .::|:...|   ..:||.|.||.|||..::|.|||..||.|.||.|:|:||||
Zfish   438 LHNIGFAIYEV-PKEMKGNQQQQLSKDFFLYNASTARSKSYVNIREVTERFCLKPGEYVIIPSTF 501

  Fly   704 DPNEEGEFIIRVFSETRNNMEENDDEVGFGETD----DRIAPSLPPPTPKEEDDPQRIALRRLFD 764
            ||::|.||::|||||.|:..:..|.|:   |.:    |.:...:.|  .:||:..:....|.:|.
Zfish   502 DPHKESEFLLRVFSENRSTSKVIDAEI---EAEPMQVDNVKKKIKP--FEEEETEEEKQFRAIFQ 561

  Fly   765 SVAGSDEEVDWQELKRILDHSM-RDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDI 828
            .:||.|.|::..||:.:|:..: :...:.::|||.::.|||:|:||.|.:|.|..:||:.|...|
Zfish   562 QIAGDDMEINANELRTVLNRVVAKHKELKTEGFSLESCRSMIALLDTDGTGHLNLQEFKHLWNKI 626

  Fly   829 AKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSREGQIPFDDFLMCAIKV 893
            .:|:.||..:||.::.:|..|.:|.||..||:.|||:|.:.:..||.:...::.||.::.|.:::
Zfish   627 KQWKLVFTRFDTDKSSTISSFEMRNALTEAGFQLNNQLYDIICMRYANEHMELDFDSYISCLVRL 691

  Fly   894 RTFIEMFRERDTDNSDTAFFNLDDWLERTIYS 925
            ......||..|.|.......|:.:||:.|:|:
Zfish   692 EGMFRAFRAFDKDGDGLIKLNVFEWLQLTMYA 723

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpB	NP_001246706.1	PD-C2-AF1	33..225	CDD:286403	5/24 (21%)
		Drf_FH1	37..189	CDD:283903
		Peptidase_C2	260..556	CDD:279042	155/304 (51%)
		Calpain_III	574..721	CDD:279416	72/149 (48%)
		EF-hand_7	759..825	CDD:290234	23/66 (35%)
		EFh	761..826	CDD:298682	23/65 (35%)
		FRQ1	827..>908	CDD:227455	26/80 (33%)
capn3b	NP_001230250.1	Peptidase_C2	66..358	CDD:279042	155/304 (51%)
		Calpain_III	372..519	CDD:279416	72/149 (48%)
		EFh	555..623	CDD:298682	23/67 (34%)
		EFh	599..654	CDD:238008	22/54 (41%)
		EFh	630..719	CDD:298682	27/88 (31%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	358	1.000	Domainoid score	I921
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	656	1.000	Inparanoid score	I755
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm6388
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.870