Protein Alignment Octbeta3R and mAChR-B

DIOPT Version :10

• Sequence 1: NP_001034048.2
• Gene: Octbeta3R / 3885573
• FlyBaseID: FBgn0250910
• Length: 1256
• Species: Drosophila melanogaster
• Sequence 2: NP_649764.1
• Gene: mAChR-B / 40955
• FlyBaseID: FBgn0037546
• Length: 1019
• Species: Drosophila melanogaster
• Alignment Length: 1296
• Identity: 265/1296 - (20%)
• Similarity: 409/1296 - (31%)
• Gaps: 476/1296 - (36%)

  Fly    63 SSLPITSQF-----VDASLTSLSLTATSSDASYSSPFSSYLSSDSTFELLSTVGPNITANGSDIA 122
            :|.|:..|.     .|.:.|:|.|...:..:...|..|. .||||  |:|..|.|...
  Fly    75 NSWPVNEQLQWYRNFDVNNTNLLLAVRNQSSDGGSTMSG-SSSDS--EILGPVLPPFA------- 129

  Fly   123 VDNQAELEESWLDLSLLLLKGFIFSSIILAAVLGNALVIISVQRNRKLRVITNYFVVSLAMADML 187
                         |...:|.....:..|:..|.||.||:::...:|.:|..:|||:.|||..|||
  Fly   130 -------------LWQTILIAICLAICIILTVGGNILVLLAFIVDRNIRQPSNYFIASLAATDML 181

  Fly   188 VALCAMTFNASVELSGGKWMFGPFMCNVYNSLDVYFSTASILHLCCISVDRYYAIVRPLEYPLNM 252
            :...:|.| .::.:..|.|..||.:|:::.|:|......|...:..|::|||.::....:|....
  Fly   182 IGTVSMPF-YTIYVLKGYWDLGPMLCDLWLSVDYTVCLVSQYTVLLITIDRYCSVKIAAKYRSWR 245

  Fly   253 THKTVCFMLANVWILPALISFTPIFLGWYTTEEHLR-EISLHPDQCS--FVVNKAY--ALISSSV 312
            |...|.:|:...||:|||:.|..|| ||    ||.. :..|.|.||:  |:.:..:  |||..
  Fly   246 TRTRVIYMVTITWIIPALLFFISIF-GW----EHFTGKRDLLPGQCAVQFLKDPIFNTALIIG-- 303

  Fly   313 SFWIPGIVMLVMYWRIFKEA--------IRQRKALSRTSSNILLNSVHMGHTQQPTSLSYLHPSD 369
            .:|...||:.|:|..|:|.|        .:|||..|..:.:....|...||.   ..:..:....
  Fly   304 YYWTTLIVLFVLYAGIYKTAYDMQKRSEAKQRKMQSMVALSAGAMSGMAGHA---AGIGVIEEKI 365

  Fly   370 CDLNATSAREET--HSALSNLEDMLQPATDEDDDRDECDELRVPSPPPRRLSRSSIDLRDLEQER 432
            .......|.::|  .||.:.:...|..:...:......:|:...:|..||.|.:.|.....::|.
  Fly   366 LKTKVELAGDQTDLDSACTTVIKRLSGSGQANPLAVATEEVEKMTPEQRRASAAKIQEEAKKREA 430

  Fly   433 YEKVTHTDSAPSMMALQQQQPSHNQLQPPAPVFNPQIWTEGKMIPSKELDKEHSHPN-----GPQ 492
            ......::.:.|......::.|.||.|              ::|..::|:......:     |.|
  Fly   431 AVDAEKSERSSSPAFDSDEESSVNQAQ--------------QLITQQKLNNMRKRSSIGLVFGAQ 481

  Fly   493 QQLSLTSGSGNSEPEPESTAYRVFGGLNSDESEGNDLYDTHLPLAEDNKELKRLIEDNYLYFKRQ 557
            ..|..|.|.||.:   :||.       ||...|....|..|.....:...|:|
  Fly   482 AALLATRGKGNLQ---KSTT-------NSKSIEAMHQYHHHQQHHHNQSPLQR------------ 524

  Fly   558 TGGTIISGPGGGKYAALSETDFIRLKAGAAACGRKAFASSDSEFLRTISESRALPEQPVPGKEKG 622
                                                 |.|..|.
  Fly   525 -------------------------------------AQSKEEM--------------------- 531

  Fly   623 FNILSLLSKTKRSSTECFTLEKKRHQANSEGSSFFRRSRNRKLSHSYNGCGGGKERKLERRQRQH 687
                                                    |.|.|..|           ::|..|
  Fly   532 ----------------------------------------RSLHHHQN-----------QQQPHH 545

  Fly   688 SDTDSTPNKPDILLDINVLSEQSGASVIQQFSDGVQLIDFSELKTPPERIRSDDELAQL------ 746
            ....:.||.|   ||   ..:::..|.:.|.::..:|:|   |..||..:.:.|.|:.:
  Fly   546 QHHQNQPNPP---LD---RPKRTSCSTLSQIAEHDRLVD---LSAPPTLLNTSDPLSPVDLAPIE 601

  Fly   747 --ADCFGESPQQPATPPPS-------LSPPELPEPSGLLIASSSELAEIFRSLSFPLGRPAGAPQ 802
              :|...:...|...|||.       ||......|.|    :||..:|:..:.............
  Fly   602 VPSDQVHQGLVQTILPPPDAFQCPTPLSDDYSDRPFG----NSSGNSELALTYDLMTNSELRYMD 662

  Fly   803 RLSTLSDQVCANYLMSPPNT---------PAPPAISVPNGGAMDSSAS-------SNAQSASIN- 850
            ..|.:...|.||...||.::         |.|||...|....::.|.|       |.:||.|:|
  Fly   663 ESSAMLASVTANSTTSPNDSVQGKPPVLPPPPPARRNPPKSNLNQSLSQTPSQNQSPSQSLSLNL 727

  Fly   851 -------------------------------------------VYFLSPP-------PHAAAPGY 865
                                                       :..|..|       ||..|.
  Fly   728 NPNHNQSQSQSIEEAVAIEKRLLVSYTGIEDFAKVRRESCIEAICLLDVPGKLAADCPHRRAS-- 790

  Fly   866 TPSDTSTVSLDVVTSLPMPVPVPQPNPQMASQSNISPKPEIILDSTLSPVEGCGDEHRDVTSPL- 929
            :|.:||  |.|.:...||| |:|           :|||.:.....|.:|....|     ||.|:
  Fly   791 SPMETS--SKDAILYSPMP-PMP-----------LSPKVKTSTPQTGTPQSAAG-----VTPPVP 836

  Fly   930 ------FKRKDSAGDADVSVSG-NGGAGGVGGVGGRQGRCSILAGYDGIQTVRKRQASVVTYDVN 987
                  .:..||..:...|.|| ....||||| ||.|         ||:...:|....   .|.|
  Fly   837 LERIEERETSDSNTNKMPSTSGTTSSTGGVGG-GGEQ---------DGVGATKKNAGD---DDAN 888

  Fly   988 VINFS---QENSDSRSYIPMGRVSTSSAKHEFSNKSSLIRRGGICIFVDEEEAEIIEQRPRGITF 1049
            ....|   ..||..|::|      .|..|| |.:|.:|
  Fly   889 REEKSLAASRNSSKRAFI------HSIGKH-FKSKKAL--------------------------- 919

  Fly  1050 AAVPSPLPKCPLCGADISSTTGTTANATATANADSTIDTTVTTSSKRSIHEQTPDLGQRPASSSS 1114
                 ||    :.|                                         :|.|..|
  Fly   920 -----PL----ILG-----------------------------------------VGGRQKS--- 931

  Fly  1115 STRFWHKRTAAVTACWQQSKNRKRRFKTGCSHCGATGGSVRPAKGWKAEHKAARTLGIIMGVFLL 1179
                             :|:||.|                          ||.||:..|:|.|:.
  Fly   932 -----------------KSENRAR--------------------------KAFRTISFILGCFVA 953

  Fly  1180 CWLPFFLWYVITSLC-GPACPCPDVLVVVLFWIGYFNSTLNPLIYAYFNRDFREAFRNTLE 1239
            ||.|:.:..::...| .|.| ..:.|.:..:::.|.||.:||..||..|:.|::.|...|:
  Fly   954 CWTPYHVLALVEGFCRHPPC-INEHLYMFSYFLCYANSPMNPFCYALANQQFKKTFTRILK 1013

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Octbeta3R	NP_001034048.2	7tmA_DmOct-betaAR-like	142..>338	CDD:320194	65/208 (31%)
		TM helix 1	142..167	CDD:320194	6/24 (25%)
		TM helix 2	174..200	CDD:320194	11/25 (44%)
		TM helix 3	213..243	CDD:320194	8/29 (28%)
		TM helix 4	255..278	CDD:320194	9/22 (41%)
		TM helix 5	302..331	CDD:320194	9/30 (30%)
		7tm_GPCRs	<1161..1234	CDD:475119	23/73 (32%)
		TM helix 6	1167..1189	CDD:410628	8/21 (38%)
		TM helix 7	1202..1227	CDD:410628	8/24 (33%)
mAChR-B	NP_649764.1	7tmA_mAChR_GAR-2-like	147..>323	CDD:320429	61/183 (33%)
		TM helix 2	168..194	CDD:320429	11/26 (42%)
		TM helix 3	206..236	CDD:320429	8/29 (28%)
		TM helix 4	248..271	CDD:320429	10/23 (43%)
		TM helix 5	293..322	CDD:320429	9/30 (30%)
		7tm_GPCRs	<932..1008	CDD:475119	27/102 (26%)
		TM helix 6	941..963	CDD:410628	8/21 (38%)
		TM helix 7	976..1001	CDD:410628	8/24 (33%)