Protein Alignment smid and AFG2

DIOPT Version :9

• Sequence 1: NP_523959.2
• Gene: smid / 38824
• FlyBaseID: FBgn0016983
• Length: 944
• Species: Drosophila melanogaster
• Sequence 2: NP_013501.1
• Gene: AFG2 / 851113
• SGDID: S000004389
• Length: 780
• Species: Saccharomyces cerevisiae
• Alignment Length: 943
• Identity: 284/943 - (30%)
• Similarity: 420/943 - (44%)
• Gaps: 249/943 - (26%)

  Fly    66 SESYNLDKVSSSEEDCVSEDSE----------PPP---------TNSVMNNMMNSLYSQPRKPLA 111
            |.|.:..|.|:|.....::.|:          |.|         |..:..|:::||...|.....
Yeast     6 SSSGSKKKSSASSNSADAKASKFKLPAEFITRPHPSKDHGKETCTAYIHPNVLSSLEINPGSFCT 70

  Fly   112 PKPISEP---IDISSGDENEDDSNTKTTN-----------GDGV---AAAAAPPPPTPAVQGS-- 157
            ...|.|.   :...:|||.....|..|.:           ||.:   .|...||..|....||
Yeast    71 VGKIGENGILVIARAGDEEVHPVNVITLSTTIRSVGNLILGDRLELKKAQVQPPYATKVTVGSLQ 135

  Fly   158 ---ALKRLMEEVPEIAVAAKKVAKPNTIHVSSSEAIQKLHQVVGNRA-----KNLSEDAVPR-SK 213
               .|:.:.|:|.:..:....|..|..|       .|.|....|:.:     .:.|:|::|. |:
Yeast   136 GYNILECMEEKVIQKLLDDSGVIMPGMI-------FQNLKTKAGDESIDVVITDASDDSLPDVSQ 193

  Fly   214 DHRNVPGLYQQL-----------------HQNQSRDRLRKFKRDLEVQHPTESFRDIGGMD---S 258
            ...|:..:|..|                 |...|::  .:..|...:..|. |:..:||:|   .
Yeast   194 LDLNMDDMYGGLDNLFYLSPPFIFRKGSTHITFSKE--TQANRKYNLPEPL-SYAAVGGLDKEIE 255

  Fly   259 TLKELCEMLIHIKSPEFYFQLGLLPSRGLLLHGPPGCGKTFLARAISGQLKMPLMEIPATELIGG 323
            :||...|:.:|  .|..:...|:.|.||:|||||||.|||.|.|.::......::.|....::..
Yeast   256 SLKSAIEIPLH--QPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSK 318

  Fly   324 ISGESEERIREVFDQAIGYSPCVLFIDEIDAIGGNRQWASKD---MERRIVSQLISSLDNLKANE 385
            ..||:|..:|::|::|..|.|.::||||||:|..||  |:.|   :|.|:|:.|::.:|.:.|
Yeast   319 YLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNR--ANDDSGEVESRVVATLLTLMDGMGA-- 379

  Fly   386 FGQSVVVIAATTRPDVLDPGLRRIGRFDHEIAIHIPSRKERREILRIQCEGLSVD------PKLN 444
             ...|||||||.||:.:||.|||.||||.|:.|.||....|.:||..|...:|.|      ..:.
Yeast   380 -AGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIK 443

  Fly   445 YDKIAELTPGYVGADLMALVSRAASVAVKRRSMKKFRELHAASEKNMTTVTLDDDEPSEDAGETP 509
            |  ||..|.|||||||.||.         |.|:.|..:....::.|:...:|
Yeast   444 Y--IASKTHGYVGADLTALC---------RESVMKTIQRGLGTDANIDKFSL------------- 484

  Fly   510 VPDSKGEETAKDAEAEQKVDGDKETSAKDKSEGDSPNIETPKKATNGNSSIKSPQKTPKKSAEKP 574
                  :.|.||.|:
Yeast   485 ------KVTLKDVES-------------------------------------------------- 493

  Fly   575 TDAAMDVDNVAPEEPKKAVEQEVDSSSSNDEYYEPTLAELTNFLDNPPEEFADPNFCLTLIDFVD 639
              |.:|:
Yeast   494 --AMVDI---------------------------------------------------------- 498

  Fly   640 AIKVMQPSAKREGFITVPDTTWDDIGALEKIREELKLAVLAPVKYPEMLERLGLTAPSGVLLCGP 704
                 :|||.||.|:.:|...|.|||..|:::.::|..:..|::..|...|||::||.||||.||
Yeast   499 -----RPSAMREIFLEMPKVYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGP 558

  Fly   705 PGCGKTLLAKAIANEAGINFISVKGPELMNMYVGESERAVRACFQRARNSAPCVIFFDEFDSLCP 769
            |||.|||.|||:|.|:||||::|||||:.|.||||||||:|..|::||::||.:|||||.|:|.|
Yeast   559 PGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSP 623

  Fly   770 KRSDGGDGNNSGTRIVNQLLTEMDGVEERKGVYILAATNRPDIIDPAILRPGRLDTILYVGFPEQ 834
            .|.  |...::...::..||.|:|||||.|||.|:|||||||.||.|:|||||||..:|||.|:.
Yeast   624 DRD--GSSTSAANHVLTSLLNEIDGVEELKGVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDV 686

  Fly   835 SERTEILKATTKNGKRPVLADDVDLDEIAAQTEGYTGADLAGLVKQASMFSLRQSLNNGDTNLDD 899
            :.|.||||..||  |.......|||.|:|.:||||:||::..|.::|.:.::.:       :||.
Yeast   687 NARLEILKKCTK--KFNTEESGVDLHELADRTEGYSGAEVVLLCQEAGLAAIME-------DLDV 742

  Fly   900 LCVRSQHFQEALQQLRPSVNEQDRKIYDKLRLK 932
            ..|..:||::|.:.:...:..:....|::..|:
Yeast   743 AKVELRHFEKAFKGIARGITPEMLSYYEEFALR 775

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
smid	NP_523959.2	Nucleolin_bd	2..71	CDD:293330	2/4 (50%)
		SpoVK	271..919	CDD:223540	226/656 (34%)
		AAA	287..418	CDD:278434	57/133 (43%)
		P-loop_NTPase	679..>716	CDD:304359	21/36 (58%)
		AAA	699..830	CDD:278434	80/130 (62%)
AFG2	NP_013501.1	CDC48	51..773	CDD:273521	274/894 (31%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0464
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000137
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.810