Protein Alignment smid and AFG2

DIOPT Version :10

• Sequence 1: NP_523959.2
• Gene: smid / 38824
• FlyBaseID: FBgn0016983
• Length: 944
• Species: Drosophila melanogaster
• Sequence 2: NP_013501.1
• Gene: AFG2 / 851113
• SGDID: S000004389
• Length: 780
• Species: Saccharomyces cerevisiae
• Alignment Length: 946
• Identity: 285/946 - (30%)
• Similarity: 419/946 - (44%)
• Gaps: 255/946 - (26%)

  Fly    66 SESYNLDKVSSSEEDCVSEDSE----------PPP---------TNSVMNNMMNSLYSQPRKPLA 111
            |.|.:..|.|:|.....::.|:          |.|         |..:..|:::||...|.....
Yeast     6 SSSGSKKKSSASSNSADAKASKFKLPAEFITRPHPSKDHGKETCTAYIHPNVLSSLEINPGSFCT 70

  Fly   112 PKPISEP---IDISSGDENEDDSNTKTTN-----------GDGV---AAAAAPPPPTPAVQGS-- 157
            ...|.|.   :...:|||.....|..|.:           ||.:   .|...||..|....||
Yeast    71 VGKIGENGILVIARAGDEEVHPVNVITLSTTIRSVGNLILGDRLELKKAQVQPPYATKVTVGSLQ 135

  Fly   158 ---ALKRLMEEVPEIAVAAKKVAKPNTIHVSSSEAIQKLHQVVGNRA-----KNLSEDAVPR-SK 213
               .|:.:.|:|.:..:....|..|..|       .|.|....|:.:     .:.|:|::|. |:
Yeast   136 GYNILECMEEKVIQKLLDDSGVIMPGMI-------FQNLKTKAGDESIDVVITDASDDSLPDVSQ 193

  Fly   214 DHRNVPGLYQQLHQNQSRDRL---------RKFKRDLEVQHPTE-----------SFRDIGGMD- 257
            ...|:..:|..|      |.|         ||....:.....|:           |:..:||:|
Yeast   194 LDLNMDDMYGGL------DNLFYLSPPFIFRKGSTHITFSKETQANRKYNLPEPLSYAAVGGLDK 252

  Fly   258 --STLKELCEMLIHIKSPEFYFQLGLLPSRGLLLHGPPGCGKTFLARAISGQLKMPLMEIPATEL 320
              .:||...|:.:|  .|..:...|:.|.||:|||||||.|||.|.|.::......::.|....:
Yeast   253 EIESLKSAIEIPLH--QPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSI 315

  Fly   321 IGGISGESEERIREVFDQAIGYSPCVLFIDEIDAIGGNRQWASKD---MERRIVSQLISSLDNLK 382
            :....||:|..:|::|::|..|.|.::||||||:|..||  |:.|   :|.|:|:.|::.:|.:.
Yeast   316 VSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNR--ANDDSGEVESRVVATLLTLMDGMG 378

  Fly   383 ANEFGQSVVVIAATTRPDVLDPGLRRIGRFDHEIAIHIPSRKERREILRIQCEGLSVD------P 441
            |   ...|||||||.||:.:||.|||.||||.|:.|.||....|.:||..|...:|.|      .
Yeast   379 A---AGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSE 440

  Fly   442 KLNYDKIAELTPGYVGADLMALVSRAASVAVKRRSMKKFRELHAASEKNMTTVTLDDDEPSEDAG 506
            .:.|  ||..|.|||||||.||.         |.|:.|..:....::.|:...:|
Yeast   441 AIKY--IASKTHGYVGADLTALC---------RESVMKTIQRGLGTDANIDKFSL---------- 484

  Fly   507 ETPVPDSKGEETAKDAEAEQKVDGDKETSAKDKSEGDSPNIETPKKATNGNSSIKSPQKTPKKSA 571
                     :.|.||.|:
Yeast   485 ---------KVTLKDVES----------------------------------------------- 493

  Fly   572 EKPTDAAMDVDNVAPEEPKKAVEQEVDSSSSNDEYYEPTLAELTNFLDNPPEEFADPNFCLTLID 636
                 |.:|:
Yeast   494 -----AMVDI------------------------------------------------------- 498

  Fly   637 FVDAIKVMQPSAKREGFITVPDTTWDDIGALEKIREELKLAVLAPVKYPEMLERLGLTAPSGVLL 701
                    :|||.||.|:.:|...|.|||..|:::.::|..:..|::..|...|||::||.||||
Yeast   499 --------RPSAMREIFLEMPKVYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLL 555

  Fly   702 CGPPGCGKTLLAKAIANEAGINFISVKGPELMNMYVGESERAVRACFQRARNSAPCVIFFDEFDS 766
            .|||||.|||.|||:|.|:||||::|||||:.|.||||||||:|..|::||::||.:|||||.|:
Yeast   556 YGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDA 620

  Fly   767 LCPKRSDGGDGNNSGTRIVNQLLTEMDGVEERKGVYILAATNRPDIIDPAILRPGRLDTILYVGF 831
            |.|.|.  |...::...::..||.|:|||||.|||.|:|||||||.||.|:|||||||..:|||.
Yeast   621 LSPDRD--GSSTSAANHVLTSLLNEIDGVEELKGVVIVAATNRPDEIDAALLRPGRLDRHIYVGP 683

  Fly   832 PEQSERTEILKATTKNGKRPVLADDVDLDEIAAQTEGYTGADLAGLVKQASMFSLRQSLNNGDTN 896
            |:.:.|.||||..||  |.......|||.|:|.:||||:||::..|.::|.:.::.:       :
Yeast   684 PDVNARLEILKKCTK--KFNTEESGVDLHELADRTEGYSGAEVVLLCQEAGLAAIME-------D 739

  Fly   897 LDDLCVRSQHFQEALQQLRPSVNEQDRKIYDKLRLK 932
            ||...|..:||::|.:.:...:..:....|::..|:
Yeast   740 LDVAKVELRHFEKAFKGIARGITPEMLSYYEEFALR 775

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
smid	NP_523959.2	Nucleolin_bd	2..71	CDD:465247	2/4 (50%)
		CDC48	<242..933	CDD:273521	237/714 (33%)
AFG2	NP_013501.1	CDC48	51..773	CDD:273521	275/897 (31%)