Protein Alignment smid and Spast

DIOPT Version :10

• Sequence 1: NP_523959.2
• Gene: smid / 38824
• FlyBaseID: FBgn0016983
• Length: 944
• Species: Drosophila melanogaster
• Sequence 2: NP_001156342.1
• Gene: Spast / 50850
• MGIID: 1858896
• Length: 614
• Species: Mus musculus
• Alignment Length: 649
• Identity: 170/649 - (26%)
• Similarity: 254/649 - (39%)
• Gaps: 156/649 - (24%)

  Fly   301 ARAISGQLKMPLMEIPATELIGGISGESEERIREVFDQAIGYSPCVLFIDEIDAIGGNRQ---WA 362
            |:..||....|....|...   |..||:|. :|....||..|....|.|||.:..|...|   |.
Mouse    87 AKRSSGTAPAPASPSPPEP---GPGGEAES-VRVFHKQAFEYISIALRIDEEEKAGQKEQAVEWY 147

  Fly   363 SKDMERRIVSQLISSLDNLKANEFGQSVVVIAATTRPDVLDPGLRRIGRFDHEIAIHIPSRKERR 427
            .|.:|..               |.|.:|:|.....:       ..|..|...::..::...|:|.
Mouse   148 KKGIEEL---------------EKGIAVIVTGQGEQ-------YERARRLQAKMMTNLVMAKDRL 190

  Fly   428 EILRIQCEGLSVDPKLNYDKIAELTPGYVGADLMALVSRAASVAVKRRSMKKFRELHAASEKNMT 492
            ::|.      .:.|.|.:.|  ..|..|                                 ...|
Mouse   191 QLLE------KLQPVLQFSK--SQTDVY---------------------------------NEST 214

  Fly   493 TVTLDDDEPSEDAGETPVPDSKGEETAKD--AEAEQKVDGDKETSAKDKSEGDSPNIETPKKATN 555
            .:|..:.....::|..|        ..||  ..|...:...| |..|..|.|.|.:...|  :.:
Mouse   215 NLTCRNGHLQSESGAVP--------KRKDPLTHASNSLPRSK-TVLKSGSAGLSGHHRAP--SCS 268

  Fly   556 GNSSIK------SPQKTPKKSAEKPTDAAMDVDNVAPEEPKKAVEQEVDSSSSNDEYYEPTLAEL 614
            |.|.:.      .|..|..|...||....      .|..|..||.::.|               |
Mouse   269 GLSMVSGARPGPGPAATTHKGTPKPNRTN------KPSTPTTAVRKKKD---------------L 312

  Fly   615 TNFLDNPPEEFADPNFC-LTLIDFVD---AIKVMQPSAKREGFITVPDTTWDDIGALEKIREELK 675
            .||.:      .|.|.. |.:.:.||   |:|                  :|||...|..::.|:
Mouse   313 KNFRN------VDSNLANLIMNEIVDNGTAVK------------------FDDIAGQELAKQALQ 353

  Fly   676 LAVLAPVKYPEMLERLGLTAPS-GVLLCGPPGCGKTLLAKAIANEAGINFISVKGPELMNMYVGE 739
            ..|:.|...||:.  .||.||: |:||.||||.|||:||||:|.|:...|.::....|.:.||||
Mouse   354 EIVILPSLRPELF--TGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGE 416

  Fly   740 SERAVRACFQRARNSAPCVIFFDEFDSLCPKRSDGGDGNNSGTRIVNQLLTEMDGVEER--KGVY 802
            .|:.|||.|..||...|.:||.||.|||..:|.:|  .:::..|:..:.|.|.|||:..  ..|.
Mouse   417 GEKLVRALFAVARELQPSIIFIDEVDSLLCERREG--EHDASRRLKTEFLIEFDGVQSAGDDRVL 479

  Fly   803 ILAATNRPDIIDPAILRPGRLDTILYVGFPEQSERTEILKATTKNGKRPVLADDVDLDEIAAQTE 867
            ::.|||||..:|.|:||  |....:||..|.:..|..:||........|:  ...:|.::|..|:
Mouse   480 VMGATNRPQELDEAVLR--RFIKRVYVSLPNEETRLLLLKNLLCKQGSPL--TQKELAQLARMTD 540

  Fly   868 GYTGADLAGLVKQASMFSLR-----QSLNNGDTNLDDLCVRSQHFQEALQQLRPSVNEQDRKIY 926
            ||:|:||..|.|.|::..:|     |..|...:.:.:  :|...|.|:|::::.||:.|..:.|
Mouse   541 GYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRN--IRLSDFTESLKKIKRSVSPQTLEAY 602

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
smid	NP_523959.2	Nucleolin_bd	2..71	CDD:465247
		CDC48	<242..933	CDD:273521	170/649 (26%)
Spast	NP_001156342.1	Required for interaction with RTN1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..298		57/294 (19%)
		Required for midbody localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..192		30/130 (23%)
		Required for interaction with ATL1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..78
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..48
		Required for nuclear localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..48
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	48..85
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	57..65
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	91..112		7/23 (30%)
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000250|UniProtKB:Q9UBP0	110..194		23/106 (22%)
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	112..198		23/114 (20%)
		MIT_spastin	114..193	CDD:239142	21/100 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	220..306		21/102 (21%)
		Sufficient for microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	226..614		132/443 (30%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	268..326		17/84 (20%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	307..310		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	308..310		0/1 (0%)
		RecA-like_spastin	341..504	CDD:410932	68/168 (40%)
		AAA_lid_3	531..585	CDD:465537	16/55 (29%)
		Vps4_C	<578..610	CDD:462762	8/25 (32%)