DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment smid and Spast

DIOPT Version :9

Sequence 1:	NP_523959.2	Gene:	smid / 38824	FlyBaseID:	FBgn0016983	Length:	944	Species:	Drosophila melanogaster
Sequence 2:	NP_001156342.1	Gene:	Spast / 50850	MGIID:	1858896	Length:	614	Species:	Mus musculus

Alignment Length:	649	Identity:	170/649 - (26%)
Similarity:	254/649 - (39%)	Gaps:	156/649 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly   301 ARAISGQLKMPLMEIPATELIGGISGESEERIREVFDQAIGYSPCVLFIDEIDAIGGNRQ---WA 362
            |:..||....|....|...   |..||:|. :|....||..|....|.|||.:..|...|   |.
Mouse    87 AKRSSGTAPAPASPSPPEP---GPGGEAES-VRVFHKQAFEYISIALRIDEEEKAGQKEQAVEWY 147

  Fly   363 SKDMERRIVSQLISSLDNLKANEFGQSVVVIAATTRPDVLDPGLRRIGRFDHEIAIHIPSRKERR 427
            .|.:|..               |.|.:|:|.....:       ..|..|...::..::...|:|.
Mouse   148 KKGIEEL---------------EKGIAVIVTGQGEQ-------YERARRLQAKMMTNLVMAKDRL 190

  Fly   428 EILRIQCEGLSVDPKLNYDKIAELTPGYVGADLMALVSRAASVAVKRRSMKKFRELHAASEKNMT 492
            ::|.      .:.|.|.:.|  ..|..|                                 ...|
Mouse   191 QLLE------KLQPVLQFSK--SQTDVY---------------------------------NEST 214

  Fly   493 TVTLDDDEPSEDAGETPVPDSKGEETAKD--AEAEQKVDGDKETSAKDKSEGDSPNIETPKKATN 555
            .:|..:.....::|..|        ..||  ..|...:...| |..|..|.|.|.:...|  :.:
Mouse   215 NLTCRNGHLQSESGAVP--------KRKDPLTHASNSLPRSK-TVLKSGSAGLSGHHRAP--SCS 268

  Fly   556 GNSSIK------SPQKTPKKSAEKPTDAAMDVDNVAPEEPKKAVEQEVDSSSSNDEYYEPTLAEL 614
            |.|.:.      .|..|..|...||....      .|..|..||.::.|               |
Mouse   269 GLSMVSGARPGPGPAATTHKGTPKPNRTN------KPSTPTTAVRKKKD---------------L 312

  Fly   615 TNFLDNPPEEFADPNFC-LTLIDFVD---AIKVMQPSAKREGFITVPDTTWDDIGALEKIREELK 675
            .||.:      .|.|.. |.:.:.||   |:|                  :|||...|..::.|:
Mouse   313 KNFRN------VDSNLANLIMNEIVDNGTAVK------------------FDDIAGQELAKQALQ 353

  Fly   676 LAVLAPVKYPEMLERLGLTAPS-GVLLCGPPGCGKTLLAKAIANEAGINFISVKGPELMNMYVGE 739
            ..|:.|...||:.  .||.||: |:||.||||.|||:||||:|.|:...|.::....|.:.||||
Mouse   354 EIVILPSLRPELF--TGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGE 416

  Fly   740 SERAVRACFQRARNSAPCVIFFDEFDSLCPKRSDGGDGNNSGTRIVNQLLTEMDGVEER--KGVY 802
            .|:.|||.|..||...|.:||.||.|||..:|.:|  .:::..|:..:.|.|.|||:..  ..|.
Mouse   417 GEKLVRALFAVARELQPSIIFIDEVDSLLCERREG--EHDASRRLKTEFLIEFDGVQSAGDDRVL 479

  Fly   803 ILAATNRPDIIDPAILRPGRLDTILYVGFPEQSERTEILKATTKNGKRPVLADDVDLDEIAAQTE 867
            ::.|||||..:|.|:||  |....:||..|.:..|..:||........|:  ...:|.::|..|:
Mouse   480 VMGATNRPQELDEAVLR--RFIKRVYVSLPNEETRLLLLKNLLCKQGSPL--TQKELAQLARMTD 540

  Fly   868 GYTGADLAGLVKQASMFSLR-----QSLNNGDTNLDDLCVRSQHFQEALQQLRPSVNEQDRKIY 926
            ||:|:||..|.|.|::..:|     |..|...:.:.:  :|...|.|:|::::.||:.|..:.|
Mouse   541 GYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRN--IRLSDFTESLKKIKRSVSPQTLEAY 602

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
smid	NP_523959.2	Nucleolin_bd	2..71	CDD:293330
		SpoVK	271..919	CDD:223540	167/640 (26%)
		AAA	287..418	CDD:278434	28/119 (24%)
		P-loop_NTPase	679..>716	CDD:304359	20/37 (54%)
		AAA	699..830	CDD:278434	56/132 (42%)
Spast	NP_001156342.1	Required for interaction with RTN1. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..298		57/294 (19%)
		Required for midbody localization. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..192		30/130 (23%)
		Required for interaction with ATL1. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..78
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..48
		Required for nuclear localization. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..48
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	48..85
		Nuclear export signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	57..65
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	91..112		7/23 (30%)
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	110..194		23/106 (22%)
		Required for interaction with microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	112..198		23/114 (20%)
		MIT_spastin	114..193	CDD:239142	21/100 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	220..306		21/102 (21%)
		Sufficient for microtubule severing. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	226..614		132/443 (30%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	268..326		17/84 (20%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	307..310		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	308..310		0/1 (0%)
		P-loop_NTPase	339..>395	CDD:304359	27/57 (47%)
		AAA	372..508	CDD:214640	59/139 (42%)
		AAA	376..506	CDD:278434	57/133 (43%)
		Vps4_C	<578..610	CDD:286426	8/25 (32%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0464
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810