DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF4 and FGD3

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_001077005.1	Gene:	FGD3 / 89846	HGNCID:	16027	Length:	725	Species:	Homo sapiens

Alignment Length:	605	Identity:	134/605 - (22%)
Similarity:	238/605 - (39%)	Gaps:	140/605 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    50 DSNNSSPIGSPNPDAGKRLGFRRQAI----QEIISSEKSYLEQLELLMNFFVRPLKE-----QAI 105
            ||.|:..  ..:.|||.......|.:    ||::.:|::|:::|.||...|...|.:     :.|
Human   135 DSENTPQ--KADKDAGLAQHSGPQKLLHIAQELLHTEETYVKRLHLLDQVFCTRLTDAGIPPEVI 197

  Fly   106 IDCSNHTLLFGQIEMIHNLNGEFL---------RELEANMENVAHAFLKMAPFFKLYSVYAFDYR 161
            :.      :|..|..||..:|:||         .|.:.| ..:.....|:|||.|:|..|..::.
Human   198 MG------IFSNISSIHRFHGQFLLPELKTRITEEWDTN-PRLGDILQKLAPFLKMYGEYVKNFD 255

  Fly   162 GALFIIQDLISKNPVFRKFLEQTESRPEV--QRKLNSLMIVPIQRVPRYKLLLEQVLLYTSPADA 224
            .|:.::.....::|:|:..:...: :.||  ...|...|:.|:||||||:|||:. .|...|.||
Human   256 RAVGLVSTWTQRSPLFKDVVHSIQ-KQEVCGNLTLQHHMLEPVQRVPRYELLLKD-YLKRLPQDA 318

  Fly   225 -DYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQKITH 288
             |.|..:.|::.|...|:|.|..:.:.|....|:.:...| ....:||.|:..:||||.:||::.
Human   319 PDRKDAERSLELISTAANHSNAAIRKVEKMHKLLEVYEQL-GGEEDIVNPANELIKEGQIQKLSA 382

  Fly   289 K-GTEIKRYCVLMSDIFMYC-KMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGN----FKLT 347
            | ||...|:..|.:.:.:|| ..::.......|...::      :...:|.:::..|    |.:|
Human   383 KNGTPQDRHLFLFNSMILYCVPKLRLMGQKFSVREKMD------ISGLQVQDIVKPNTAHTFIIT 441

  Fly   348 CQSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCRKTLRKDSSKRTPIRKKDMKKFGA------DY 406
            .:...:...:...:..:.|:..|:..|:.|.|..:|               .|.||.      |.
Human   442 GRKRSLELQTRTEEEKKEWIQIIQATIEKHKQNSET---------------FKAFGGAFSQDEDP 491

  Fly   407 VLSPN-------------------------KRKCEYDTVFRNKNRSTDSEEETEDSACFSRKR-- 444
            .|||:                         .||....|....:.:|..|..||.:|  .:::|
Human   492 SLSPDMPITSTSPVEPVVTTEGSSGAAGLEPRKLSSKTRRDKEKQSCKSCGETFNS--ITKRRHH 554

  Fly   445 -KVASGILRAPNGNPMGKASSSSSSSVSMKR----------VAP-----PPPAPPPPPAVQMRHQ 493
             |:...::       .||.|...:.:....|          |||     .|.|.|.|..:
Human   555 CKLCGAVI-------CGKCSEFKAENSRQSRVCRDCFLTQPVAPESTEKTPTADPQPSLL----- 607

  Fly   494 PGRPGELATASKENRISKLYKKIAAGD-KVANVR-----GILKRS--------NVPAPNNDHDPS 544
               .|.|..:......|:::..|...| :|.:::     |.|.|:        :||.|....|..
Human   608 ---CGPLRLSESGETWSEVWAAIPMSDPQVLHLQGGSQDGRLPRTIPLPSCKLSVPDPEERLDSG 669

  Fly   545 YGFASRYSDSKSYFRAHNVD 564
            :.:..:::....|..|.:.:
Human   670 HVWKLQWAKQSWYLSASSAE 689

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	53/190 (28%)
		PH-like	269..>311	CDD:302622	16/43 (37%)
		PH	277..374	CDD:278594	20/102 (20%)
FGD3	NP_001077005.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..151		6/17 (35%)
		RhoGEF	160..339	CDD:238091	53/187 (28%)
		PH1_FGD3	371..478	CDD:275422	24/127 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	487..532		7/44 (16%)
		FYVE_FGD3	531..584	CDD:277279	11/61 (18%)
		PH2_FGD1-4	598..702	CDD:270056	19/100 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	703..725
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S6034
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R7163
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	6	5.750