Protein Alignment RhoGEF4 and TUS1

DIOPT Version :10

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: NP_013529.3
• Gene: TUS1 / 851145
• SGDID: S000004417
• Length: 1307
• Species: Saccharomyces cerevisiae
• Alignment Length: 530
• Identity: 99/530 - (18%)
• Similarity: 175/530 - (33%)
• Gaps: 165/530 - (31%)

  Fly    61 NPDAGKRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSN-HTLLFGQIEMIHNL 124
            ||...:|..|    |.::|..|:..|......:..:.:...:..:.|... ..|.|...|.:..|
Yeast   462 NPREVQRQSF----IFDLIILEERSLNMATAAVEIYGKRFDKSLLPDEPEFKALAFDIFEPLIQL 522

  Fly   125 NGEFLRELEANMENVAHAFLKMAPFF--------------KLYSVYA-------FDYRGALFIIQ 168
            :.|||                :.|.|              |:||.:.       .:|..|:..:.
Yeast   523 HTEFL----------------LTPIFWKLKTRGKFIDGVGKIYSKWCGEAKNIYLNYAKAMATVH 571

  Fly   169 DLI---SKNPV-FRKFLEQTESRPEVQRK------------LNSLMIVPIQRVPRYKLLLEQVLL 217
            ::|   .||.. |..:|::.::..|:.|.            ..||..:|:        .|..:|.
Yeast   572 EIIMWEKKNKTKFVTWLKEIDNSVEITRSKMYHDVIFFGGFFKSLQNMPV--------TLRSILK 628

  Fly   218 YTSPADADYKLLKESVKEIEATASHIN----TCVEEQEITQY---LIHLQNSLVNRTP------- 268
            .|.|:..||:.||..:||:|.....:|    ..::.:::.::   |:...|| .|.|.
Yeast   629 NTDPSMEDYEYLKIVIKEVEKLNFEVNQVHGLAIDHRKLVRFSKQLVLSTNS-SNATSYVNVGGS 692

  Fly   269 ---------------NIVKPSRRVIKEGVLQK---------------------ITH---KGTEIK 294
                           .:..|.|:::..|.:.|                     ||.   || |.:
Yeast   693 TNANDDDAIQDKLALGLTYPERKLVLSGTVYKKRDLWLDPTPVYIALLDNCLLITEEISKG-ETQ 756

  Fly   295 RYCVLMSDI-FMYCKMIKERAPNTVVENSLECCCIFPLKKC--KVYEMLPGNFKLTCQSDGIIFG 356
            :|.::...| ..|..:.|.:.|.|..:         ||:..  |.::....||.....|...:..
Yeast   757 KYKLIERPIPIDYLSLEKRKIPGTSKQ---------PLRNYSQKEHKSPMHNFSTPINSMRPLLK 812

  Fly   357 S---------GDVQLSRTWVGFIRDAID-LHVQCRKTLRKDSSKRTPIRKKDMKKFGADYVLSPN 411
            |         ||.:.|.|.:.......| ...:.|.|...:|.|......:.:.:: .|.::...
Yeast   813 SSGNHMSTAYGDRKTSNTEISNANPNTDEFSFKIRNTATGESFKFFTESAEVLNQW-IDAIMESF 876

  Fly   412 KRKCEYDTVFRNKNRSTDSEEET---EDSACFSRKRKVASGILRAPNGNPMG--------KASSS 465
            ||..|        |...::.|.|   .:.|.|.:...|...:  ||.|:.:.        ||:..
Yeast   877 KRNAE--------NHDLNAFEFTVLSSEFAYFDKDAPVNLPV--APEGSEIDVALKAYAQKANKD 931

  Fly   466 SSSSVSMKRV 475
            |.|.....|:
Yeast   932 SCSWSKTTRI 941

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:459876	40/207 (19%)
		PH-like	269..>311	CDD:473070	12/66 (18%)
		PH	277..374	CDD:459697	24/132 (18%)
TUS1	NP_013529.3	RhoGEF	469..655	CDD:238091	41/213 (19%)
		CNH	939..1287	CDD:214481	1/3 (33%)