DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and ROM2

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_013475.1	Gene:	ROM2 / 851086	SGDID:	S000004363	Length:	1356	Species:	Saccharomyces cerevisiae

Alignment Length:	706	Identity:	141/706 - (19%)
Similarity:	272/706 - (38%)	Gaps:	177/706 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly    15 HSP---QTPLSFSRELRQVLQERNLLTAKS----RRKVCSMLDSNN------SSPIGSPN-PDAG 65
            :||   :..|.:|....:.|:::..|..|.    :|.:...||.::      :|.:...: .:..
Yeast   590 YSPTCTRDALCYSISCPRRLEQQARLNLKPNGGLKRNISMALDDDDEEKPSWTSSVSKEDWENLP 654

  Fly    66 KRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCS------NHTLLFGQIEMIHNL 124
            |:...|::||.|:..:||::::.||:..:.|::.|.|..||...      .|  :|..|..|:::
Yeast   655 KKEIKRQEAIYEVYITEKNFVKSLEITRDTFMKTLAETNIISADIRKNFIKH--VFAHINDIYSV 717

  Fly   125 NGEFLRELEAN------MENVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQ 183
            |..||:.|...      :..:....|:..|||:.:..|......|.::|:...|.||.|.:|.:.
Yeast   718 NRRFLKALTDRQRSSPVVRGIGDIVLRFIPFFEPFVSYVASRPYAKYLIETQRSVNPYFARFDDD 782

  Fly   184 TESRPEVQRKLNSLMIVPIQRVPRYKLLLEQVLLYTSPA--DADYK-------LLKESVKEIEAT 239
            ..| ..::..::|.:...:.|..||.||:::::..|.|.  .:||:       .|::.:|.|: .
Yeast   783 MMS-SSLRHGIDSFLSQGVSRPGRYMLLVKEIMKSTDPEKDKSDYEDLSKAMDALRDFMKRID-Q 845

  Fly   240 ASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIK-EGVLQK---ITHKGT---EIKRYC 297
            ||.......:.::.:..|..:|..||...|   ..||.|| ||:|.:   ....||   :|:.|
Yeast   846 ASGAAQDRHDVKLLKQKILFKNEYVNLGLN---DERRKIKHEGILSRKELSKSDGTVVGDIQFY- 906

  Fly   298 VLMSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEM-LPGNFKLTCQSDGIIFGSGDVQ 361
             |:.::.::   :|.:|.|...::             ||::. :|......|..:       |:.
Yeast   907 -LLDNMLLF---LKAKAVNKWHQH-------------KVFQRPIPLPLLFACPGE-------DMP 947

  Fly   362 LSRTWVGFIRDAIDLHVQCRKTL----RKDSSKRTPI--------RKKDMKKFGADY-----VLS 409
            ..|.::|.       |..|..|:    ...|:.:..|        ::..:..:.|.|     :|.
Yeast   948 ALRKYIGD-------HPDCSGTVIQPEYNTSNPKNAITFLYYGAKQRYQVTLYAAQYAGLQTLLE 1005

  Fly   410 PNKR-------KCEYDTVFRNKNRSTDSEEETED-SAC-FSRKRKVA-------SGILRAPNGNP 458
            ..|:       |.|...|.:..:|..|...:... ::| ..||..:|       |.|.|..|.:.
Yeast  1006 KIKQGQAAIISKTEMFNVTKMSDRFFDYTNKINSVTSCDGGRKLLIATNSGLYMSNIKRQQNKDH 1070

  Fly   459 MGKASSSSSSSVSMKRVAPPPPAPPPPPAVQMRHQPGRPGELATASKENRISKLYKKIAAGDKVA 523
            ..|:|:..|:.:.:               ||                .|.|:    :||..::..
Yeast  1071 RHKSSAFFSTPIQL---------------VQ----------------RNNIT----QIAVLEEFK 1100

  Fly   524 NVRGILKRSNVPAPNNDHDPSYGFASRYSDSKSYFRAHNVD--NTIPTFVKREDLFNGDNMF-PV 585
            ::..::.:.....|       ........:..|:|:.|:.:  |.:..|.:.:  .||..:. ..
Yeast  1101 SIILLIDKKLYSCP-------LSLIEAEGNGTSFFKKHHKELINHVSFFAEGD--CNGKRLIVTA 1156

  Fly   586 HLKSHEEICTPPQKKRVKFDDTLDAVSPVLVEKPFAFSSESSSALKPTSLRERIYD 641
            |..||.          :|:   .:...|:|.||..:.|....|..|  .:.|.|:|
Yeast  1157 HSSSHS----------IKY---FEHEHPLLAEKNGSGSGNKKSLKK--KITEVIFD 1197

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	48/190 (25%)
		PH-like	269..>311	CDD:302622	13/48 (27%)
		PH	277..374	CDD:278594	20/104 (19%)
ROM2	NP_013475.1	ROM1	4..1356	CDD:227709	141/706 (20%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	62	1.000	Domainoid score	I2478
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm46934
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.810