Protein Alignment RhoGEF4 and plekhg5b

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_017209177.1
• Gene: plekhg5b / 796404
• ZFINID: ZDB-GENE-090908-6
• Length: 1110
• Species: Danio rerio
• Alignment Length: 741
• Identity: 150/741 - (20%)
• Similarity: 275/741 - (37%)
• Gaps: 205/741 - (27%)

  Fly    17 PQTP--LSFSRE-LRQVLQERNLLTAKSRRKVCSMLDSNNSSPIGSPNPDAGKRLGFRRQ-AIQE 77
            |:.|  |:|.:: ..:..:|.||....|.|   ::||          :|::..|..|::| ||.|
Zfish   375 PKLPPQLAFHQDSWEEEEEEPNLTLEDSWR---TLLD----------DPESLSRRQFQQQEAIWE 426

  Fly    78 IISSEKSYLEQLELLMNFF---VRPLKEQAIIDCSNHTLLFGQIEMIHNLNG-----------EF 128
            :|.:|.:|:::|.::.:.|   :..|::.:::.....|.||..|:.|..|:.           |.
Zfish   427 LIQTEATYIKKLRVITDLFLCGLLNLQDNSLLGEVEPTRLFSNIQDIVRLHCTLWVQVMFPALEK 491

  Fly   129 LRELEANME--NVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPEVQ 191
            .|...:.:|  ::...|...|..|:.|..|..:....:..::.|:..|.:|:.::...|:..:..
Zfish   492 ARGSRSLIEPTDLQEGFSTFASRFQPYIRYCMEEESCMEYMRSLLRDNELFKIYITWAETNKQCN 556

  Fly   192 R-KLNSLMIVPIQRVPRYKLLLEQVLLYT--------------------SPADADYKLLKESVKE 235
            | ||..:::.|.||:.:|.|||:.:|..|                    :..|:..:..:|..|
Zfish   557 RLKLADMLVKPHQRLTKYPLLLKTILKKTEDQASRDALNNMVACVEGFINGVDSQMRQRQEQQK- 620

  Fly   236 IEATASHINT--CVE--EQEITQYL-----IHLQNSLVNRTPNIVKPSRRVIKEGVLQKITHKGT 291
            :.||::.:.:  .||  .:|:.:.|     |.|...::..:|   :.:|::..||.|:....|.:
Zfish   621 LAATSARVESYEAVEGASEEVEKALKEFSRIDLTAPMIGTSP---EETRQLHLEGALRMKEGKDS 682

  Fly   292 EIKRYCVLMSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGNFKLTCQSDGIIFG 356
            .:..||.|.:|:.:..|.:|.                  |:|.||
Zfish   683 RMDAYCFLFTDLLLITKPVKR------------------LEKVKV-------------------- 709

  Fly   357 SGDVQLSRTWVGFIRDAIDLH-VQCRKTLRKDSSKRTPIRKKDMKKFGADYVLSPN---KRKCEY 417
                         :|..:.|| |.||:.  ||......|...:.|...|.|....|   :.|...
Zfish   710 -------------VRQPLLLHNVVCREL--KDPGSFVLIYLNEFKSAVAAYTFQANSAAQGKSWT 759

  Fly   418 DTV--FRNKNRSTDSEEETEDSACFSRK---RKVASGILRAPNGNP-------MGKASSSSSSSV 470
            |.:  .:|:.:...|||.....||..::   .:.......:.|.:|       ....|..|:.::
Zfish   760 DAICNVQNQLQRLRSEEARRQKACLKKRLLDNQEDEDSCNSTNSSPHLRHKDQSNSQSDGSTETL 824

  Fly   471 SMKRVAPPPPAPPP------------PPAVQMRHQPGRPGELATASKENRISKL---YKKIAAGD 520
            |:..:.....:..|            |.:..::..|    ||.........|.|   |:.::...
Zfish   825 SVMDMDESGLSLDPSVLHSDLGGHSDPDSAILQQNP----ELEPHLNSQDASDLDPQYRSMSMDS 885

  Fly   521 KVANV--RGILKRSNVPAP---------------------NNDHDPSYGFASRYSDSKSYFRAHN 562
            ....:  ..:|:..|..||                     .:|.:......|:.|.:.|:.:...
Zfish   886 AYGTLSPESMLETQNPAAPVQEEEKEEEDKESTETTGDEEGDDEEDKISVGSQNSITLSFNKLRR 950

  Fly   563 ---VDNTIPTFVKREDLFNGDNMFPVHLKS--HEEICTPPQKK---------RVKFDDTLDAVSP 613
               |...:| |::|   ..|.:....:|.|  |::|   |:||         |||  .|..|:|.
Zfish   951 QAPVQRRLP-FLER---LVGKSRSEDNLLSTVHQDI---PKKKIKSRHTWEQRVK--HTEPALSR 1006

  Fly   614 VLVEKPFAFSSESSSALKPTSLRERI 639
            .|.|...|.|.||    .||....|:
Zfish  1007 SLTELKAAESDES----LPTEESHRL 1028

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	44/206 (21%)
		PH-like	269..>311	CDD:302622	10/41 (24%)
		PH	277..374	CDD:278594	15/96 (16%)
plekhg5b	XP_017209177.1	None

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910