DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and fgd4b

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	XP_001332264.7	Gene:	fgd4b / 793364	ZFINID:	ZDB-GENE-110421-6	Length:	775	Species:	Danio rerio

Alignment Length:	440	Identity:	99/440 - (22%)
Similarity:	189/440 - (42%)	Gaps:	84/440 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    77 EIISSEKSYLEQLELLMNFFVRPLKEQAI-----IDCSNHTLLFGQIEMIHNLNGEF-LRELEAN 135
            |::.:|::|:.:|.||...|...|.|:|:     :|....  :|..:..|:..:.:| |.:||..
Zfish   221 ELLQTERAYVSRLHLLDQVFCVRLTEEALKGSFPVDVVKG--IFSNVGSIYTFHSQFLLPDLETR 283

  Fly   136 ME------NVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPEV--QR 192
            |.      .|.....::|||.::|:.|..::..|:.:::..:.::..|...::..:|: ||  ..
Zfish   284 MSQWDSKPRVGDVLAQLAPFLRMYAEYVKNFDSAMELLKLWMERSTQFSAIIQDIQSQ-EVCGSL 347

  Fly   193 KLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEITQYLI 257
            .|...|:.|:||||||::||:..|......|.|....::|:..|...|:|.|..:.:.|..:.|:
Zfish   348 TLQHHMLEPVQRVPRYEMLLKDYLKKLPEDDEDRSQAQKSLNIISMAATHSNMAIRKMENLKKLM 412

  Fly   258 HLQNSLVNRTPNIVKPSRRVIKEGVLQKITHKGT-EIKRYCVLMSDIFMYC----KMIKER-APN 316
            .:. .::....:||.||..:||||.:.|:..:.| .::||..|.:::.:||    .::.:| ...
Zfish   413 EIY-EMLGGEEDIVNPSNELIKEGQILKLAARNTSSMERYLFLFNNMLLYCVPKFSLVGQRFTVR 476

  Fly   317 TVVENSLECCCIFPLKKCKVYEM----LPGNFKLTCQSDGIIFGSGDVQLSRTWVGFIRDAIDLH 377
            |.|.          ::..||.|.    .|..|:::.:...:...:...|....|:...::.|::.
Zfish   477 TRVR----------VEGMKVLETSNEDYPHTFQVSGKERTLELQASSQQDKEDWIKAFQETIEIF 531

  Fly   378 VQCRKTL------------RKDSSKRTP--IRKKDM-------KKFGA-----------DYVL-- 408
            .|..:|.            :::..||.|  ||..::       :.|.|           .||:
Zfish   532 QQKNETFKSACKEATDEVSKEELGKRAPRWIRDNEVTMCMKCKEPFNALTRRRHHCRACGYVVCY 596

  Fly   409 --SPNKRKCEYDTVFRNK-----NRSTDSEEETEDSACFSRKRKVASGIL 451
              |.:|....||:...||     ........:.|:..|..:|     |||
Zfish   597 KCSDHKASLRYDSNKLNKVCKDCYHILTGRADAEEPVCGKKK-----GIL 641

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	47/180 (26%)
		PH-like	269..>311	CDD:302622	15/46 (33%)
		PH	277..374	CDD:278594	21/106 (20%)
fgd4b	XP_001332264.7	RhoGEF	221..399	CDD:214619	47/180 (26%)
		PH1_FDG4	431..524	CDD:275434	21/102 (21%)
		FYVE_FGD1_2_4	559..623	CDD:277280	13/63 (21%)
		PH2_FGD1-4	645..748	CDD:270056
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R7163
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	5	4.800