Protein Alignment RhoGEF4 and net1

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: NP_001072884.2
• Gene: net1 / 780346
• XenbaseID: XB-GENE-487220
• Length: 585
• Species: Xenopus tropicalis
• Alignment Length: 455
• Identity: 94/455 - (20%)
• Similarity: 179/455 - (39%)
• Gaps: 102/455 - (22%)

  Fly    40 KSRRKV--CSMLDSNNSSPIGSPNPDAGKRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPLKE 102
            |.|..|  ..|||.|....:.:      |.:. |::||.|:...|:..::.|:|....:..|:.:
 Frog   157 KRRNSVLWSEMLDINMKESLST------KEIK-RQEAIFEMTKGEQDLIDDLKLARKAYHDPMLK 214

  Fly   103 QAIIDCSNHTLLFGQIEMIHNLNGEFLREL------EANMENVAHAFLKMAPFFKLYSVYAFDYR 161
            .:|:.....|.:||.::....|:.|.|.:|      :..:..:....:...|....|..|..:..
 Frog   215 LSIMSEEELTQIFGDLDSYIPLHEELLAKLGEATKADGTVGQIGPILINWLPRLNAYKEYCSNQL 279

  Fly   162 GALFIIQDLISKNPVFRK---FLEQTESRPEVQRKLN--SLMIVPIQRVPRYKLLLEQVLLYTSP 221
            .|    :.|:.|..:.|:   ||::....| ..|||:  |.:.:|..|:.:|.|||:::|.:|..
 Frog   280 AA----KALLDKKKLDRRVQDFLQRCLESP-FSRKLDLWSFLDIPRSRLVKYPLLLKEILRHTPK 339

  Fly   222 ADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTPNI-VKPSRRVIKEGVLQ- 284
            ...|.|.|.|::..|:...:.||....|.| .||.|.....|.::..:: ::.|:.::..|.|:
 Frog   340 DHPDTKGLHEALCLIQGVLTDINLKKGESE-CQYYIDKLEYLDDKQKDLRIESSKALLCHGELKN 403

  Fly   285 KITHKGTEIKRYCVLMSDIFMYCKMIKERAPNTVVENSLECCCIF----PLKKCKVYEMLPGNFK 345
            |..|     |.:..|..|:.:..:        .|:.|..:...::    |::...:.::..|:.:
 Frog   404 KNGH-----KLFLFLFQDVLVLTR--------PVIRNEHQHFQVYRQPIPVQDLVLEDLQDGDVR 455

  Fly   346 LTCQSDGI---------IF-------GSG--------DVQLSRTWVGFIRDAIDLHVQCRKTLRK 386
            :.....|.         ||       |:|        ||...:.|:..|:.|:..:.|...|.:|
 Frog   456 MGGSFRGAFSNSDKAKNIFRVRFKDAGAGQSHTLQANDVFHKQQWLNCIKTAVSPYQQASPTEQK 520

  Fly   387 DSSKRTPIRKKDMKKFGADYVLSPNKRKCEYDTVFRNKNRSTDSEEETEDSACFSRKRKVASGIL 451
            :                    |.....:||     .|...:|:|::.        |:....|||:
 Frog   521 E--------------------LPDLNEECE-----ENNPPATNSKDH--------RRSSTISGIM 552

  Fly   452  451
             Frog   553  552

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	42/180 (23%)
		PH-like	269..>311	CDD:302622	8/43 (19%)
		PH	277..374	CDD:278594	20/125 (16%)
net1	NP_001072884.2	RhoGEF	186..362	CDD:366202	42/180 (23%)
		PH_Net1	376..510	CDD:270044	23/146 (16%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910