DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF4 and Arhgef12

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_081420.2	Gene:	Arhgef12 / 69632	MGIID:	1916882	Length:	1544	Species:	Mus musculus

Alignment Length:	583	Identity:	116/583 - (19%)
Similarity:	204/583 - (34%)	Gaps:	185/583 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly    71 RRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLN---------- 125
            |::.|.|:..:|::::..|::|...|.:.:..:.|:..|....:|..:|.|..|:
Mouse   789 RQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHVGLNEQMKAV 853

  Fly   126 ------------GE-----FLRELEANMENVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISK 173
                        ||     |....|..:::.|..|....||             ||.:|:....|
Mouse   854 RKRNETSVIDHIGEDLLIWFSGPGEEKLKHAAATFCSNQPF-------------ALEMIKSRQKK 905

  Fly   174 NPVFRKFLEQTESRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVLLYTS-PADADYKLLKESVKEI 236
            :..|..|::..||.|..:| :|..::...:||:.:|.|||:.:..||. |.:.:  .:|::....
Mouse   906 DSRFHTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPPERE--KVKKAADHC 968

  Fly   237 EATASHINTCVEEQEITQYLIHLQNSL------------VNRTPNIVKPSRRVIKEGVLQKITHK 289
            ....:::|..|.|.|..|.|...|..|            |:...|:....|::|.||.|....::
Mouse   969 RQILNYVNQAVREAENKQRLEDYQRRLDTSNLKLSEYPNVDELRNLDLTKRKMIHEGPLVWKVNR 1033

  Fly   290 GTEIKRYCVLMSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGNFKLTCQSDGII 354
            ...|..|.:|:.||.:..:...:|                              ..|.|.|. |:
Mouse  1034 DKSIDLYTLLLEDILVLLQKQDDR------------------------------LVLRCHSK-IL 1067

  Fly   355 FGSGDVQLSRTWVGFIRDAIDLHVQCRKTLRKDSSKRT--PIRKKD---MKKFGAD----YVLSP 410
            ..:.|                             ||.|  |:.|..   :::...|    :|:|.
Mouse  1068 ASTAD-----------------------------SKHTFSPVIKLSTVLVRQVATDNKALFVISM 1103

  Fly   411 NKRKCE-YDTVFRNKNRSTDSEEET-EDSACFSRKRKVASGILRAPNGNPMGKASSSSSSSVSMK 473
            :....: |:.|     ..|.||:.. :|..|     ::|:.:          |..|:.
Mouse  1104 SDNGAQIYELV-----AQTVSEKTVWQDLIC-----RMAASV----------KEQSTK------- 1141

  Fly   474 RVAPPPPAPPPPPAVQMRHQPGRPGELATASKENRISKLYKKIAAGDKVANVRGILKRSNVPAPN 538
                |.|.|.|||. :..:....|.:|.....:..::.|    .:.|:|..:...| .|:.|..:
Mouse  1142 ----PIPLPQPPPC-EGDNDEEEPAKLKVEHHDLSVAGL----QSPDRVLGLESPL-ISSKPQSH 1196

  Fly   539 NDHDPSYGFASRYSDSKSYFRAHNVDNTIPTFVKRED----LFNGDNMFPV-----HLKSHEE 592
            :.:.|          .||  .|.::..|...|.|.:.    |..||..:||     ||...||
Mouse  1197 SLNTP----------GKS--AAEHLFVTATQFAKEQHANGALKEGDGGYPVTIPGPHLPVSEE 1247

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	41/198 (21%)
		PH-like	269..>311	CDD:302622	11/41 (27%)
		PH	277..374	CDD:278594	15/96 (16%)
Arhgef12	NP_081420.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..62
		PDZ_signaling	71..145	CDD:238492
		RGS_LARG	350..571	CDD:188708
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	575..711
		RhoGEF	792..977	CDD:279015	41/199 (21%)
		PH_LARG	996..1133	CDD:275425	33/206 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1138..1159		7/32 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1387..1406
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1442..1469
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810