DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF4 and ARHGEF28

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_001073948.2	Gene:	ARHGEF28 / 64283	HGNCID:	30322	Length:	1731	Species:	Homo sapiens

Alignment Length:	718	Identity:	128/718 - (17%)
Similarity:	263/718 - (36%)	Gaps:	156/718 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 GFIHS-----PQTPL-SFSRELRQVLQERNLLTAKSRRKVCSMLDSNNSSP----------IGSP 60
            |.:|.     |.|.| ||.|....:..|.:..:.:||.....:|.|..|||          :.|.
Human   750 GQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSS 814

  Fly    61 -----NPDA--------------------GKRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPL 100
                 :.||                    .|.:..|:..|.|::.:|..:::.|.::...|.:.:
Human   815 LWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGM 879

  Fly   101 KEQAIIDCSNHTLLFGQIEMIHNLNGEFLREL-EANMENVAHA---FL----------------- 144
            ||:..:|.|....:|..::.:..::..|...: |...|:.|.:   |:
Human   880 KEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENA 944

  Fly   145 -KMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPEVQRK-LNSLMIVPIQRVPR 207
             ||.   |:|..:...::.|:.:.::| .:|..|:.|::...|....:|: :...:::..||:.:
Human   945 SKMK---KIYGEFCCHHKEAVNLFKEL-QQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITK 1005

  Fly   208 YKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRT----- 267
            |.:|:|::|.||.....::|.|::::..|:...:.::..|.|.|..|..:.:.|.:.|:|
Human  1006 YPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLK 1070

  Fly   268 -------PNIVKPSRRVIKEGVLQKITHKGTEIKRYCVLMSDIFMYCKMIKERAPNTVVENSLEC 325
                   ..::...|.::.:|::...|..|.......:|::|:.::.:...::.....|:.....
Human  1071 NGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSV 1135

  Fly   326 CCIFPLKKCKVYEMLPGNFKLTCQSDG---IIFGSGDVQLSRTWVGFIRDAIDLHVQCRKTLRKD 387
            ..:..|...:|.....|.|.::..|.|   ....:...:....|:..|:.|::       :..::
Human  1136 ISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVE-------SCPEE 1193

  Fly   388 SSKRTPIRKKDMKKFGA---------DYVLSPNKRKCEY-----------------DTVFRNKNR 426
            ...||....:|.:|..|         :.:.:.:::.|.|                 :.|....:.
Human  1194 KGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHL 1258

  Fly   427 --STDSEEETEDSACFSRKRKVASGILRAPNGNPMGKASSSSSSSVSMKRVAPPPPAPPPPPAVQ 489
              ..|..|..:.::..:...|.|..:..|...:.||..|.|...|.....:|         ..:.
Human  1259 LIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLA---------DTLS 1314

  Fly   490 MRHQPGRP-GELATASKENR--------ISKLYKKIA---AGDKV--ANVRGILKRSNVPAPNND 540
            ....||.| ..|.|..:|.|        |..:...:|   ||:||  .|..|..:...:.|..|.
Human  1315 SHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNL 1379

  Fly   541 HDPSYGFASRYSDSKSYFRAHNVDNTIPTFVKREDLFNGDNMF---PVH-------LKSHEEICT 595
            ....|...:..:...|:...|.:     ...::|.|..|.::.   |:.       .:.|||:..
Human  1380 TRLLYSLQAALTIQDSHIEIHRL-----VLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELAN 1439

  Fly   596 PPQ 598
            ..|
Human  1440 VHQ 1442

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	37/192 (19%)
		PH-like	269..>311	CDD:302622	6/41 (15%)
		PH	277..374	CDD:278594	14/99 (14%)
ARHGEF28	NP_001073948.2	ANK	<153..216	CDD:295348
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	287..316
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	473..524
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	630..649
		C1	653..698	CDD:237996
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	710..800		14/49 (29%)
		RhoGEF	853..1042	CDD:214619	37/192 (19%)
		PH_p190RhoGEF	1087..1187	CDD:275430	14/99 (14%)
		PH	<1123..1188	CDD:278594	10/64 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1187..1207		3/26 (12%)
		Interaction with PTK2/FAK1, required for regulation of axonal branching and synapse formation. /evidence=ECO:0000250	1295..1304		2/8 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1312..1339		7/26 (27%)
		Mediates cytoplasmic retention and interaction with YWHAH. /evidence=ECO:0000250	1372..1383		2/10 (20%)
		RNA-binding. /evidence=ECO:0000250	1496..1527
		Mediates cytoplasmic retention and interaction with MAPK8IP1. /evidence=ECO:0000250	1566..1579
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810