Protein Alignment RhoGEF4 and plekhg5a

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_009304320.1
• Gene: plekhg5a / 562861
• ZFINID: ZDB-GENE-060503-766
• Length: 1157
• Species: Danio rerio
• Alignment Length: 677
• Identity: 129/677 - (19%)
• Similarity: 237/677 - (35%)
• Gaps: 217/677 - (32%)

  Fly     5 RQKGYGNGFIHSPQTP--LSFSRELRQVLQERNLLTAKSRRKVCSMLDSNNSSPIGSPNPD-AGK 66
            :.|.|.......|:.|  |||.|:..:  :|.||...:|.:::..             ||: ..:
Zfish   383 QSKLYSYTMFGLPKMPPQLSFHRDSWE--EEPNLELEESWKQLLE-------------NPEILTR 432

  Fly    67 RLGFRRQAIQEIISSEKSYLEQLELLMNFF---VRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEF 128
            |...:::||.|::.:|..|:::|.::.:.|   :..|:|..::.....|.||..|:.|..|:...
Zfish   433 RQCHQQEAIWELLQTESDYIKKLHVITDLFLCGLLNLQESGLLTEVEPTRLFSNIQDIVQLHSSL 497

  Fly   129 LREL----------EANMENVAHAFLKMAPF---FKLYSVYAFDYRGALFIIQDLISKNPVFRKF 180
            ..|:          :..|.|........:.|   ||.|..|..:..|.:..::.|:..|.:||.:
Zfish   498 WTEVMLPALEKARQKRTMLNPTELHQGFSTFGFRFKPYIRYCMEEEGCMEYMRTLLKDNELFRIY 562

  Fly   181 LEQTESRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVL--------------------LYTSPADA 224
            :...|:..:..| ||..:::.|.||:.:|.|||:.||                    .:.:..|:
Zfish   563 VTWAETHKQCNRLKLTDMLVKPHQRLTKYPLLLKSVLKKTDDPPTREAINRMVAAVECFINSVDS 627

  Fly   225 DYKLLKESVKEIEATASHINT--CVE--EQEITQYLIHLQN-----SLVNRTPNIVKPSRRVIKE 280
            ..: .:|..:::.|.|..|..  .||  .:|:.:.|....|     .::...|   :.:|:::.|
Zfish   628 QMR-HREEKQKLAAIAGRIEAYEAVEGASEEVERILKEYNNFDLMAPVMGAPP---EETRQLLLE 688

  Fly   281 GVLQKITHKGTEIKRYCVLMSDIFMYCKMIKE-------RAPNTVVENSLECCCIFPLKKCKVYE 338
            |.|:....|.:.:..||.|.:|:.:..|.:|.       |.| .|:.|.:          |:..:
Zfish   689 GALKMKEGKESRMDVYCFLFTDVLLITKPVKRVEKVKVIRQP-LVIHNVV----------CRELK 742

  Fly   339 MLPGNFKLT------CQSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCRKTLRKDSSKRTPIRKK 397
            . ||:|.|.      |......|.:......|:|:..|.:|.:...:.|...::|.|.     :|
Zfish   743 D-PGSFLLIYLNEFGCAVASYCFQANSGAQGRSWIEAIYNAQNQLQRLRSETQRDESD-----EK 801

  Fly   398 DMKKFGADYVLSPNKRKCEYDTVFRNKNRSTDSEEETEDSACFSRKRKVASGILRAPNGNPMGKA 462
            |                                |:|||                          :
Zfish   802 D--------------------------------EDETE--------------------------S 808

  Fly   463 SSSSSSSVSMKRVAPPPPAPPPPPAVQMRHQPGRPGELATASKENRISKLYKKIAAGDKVANVRG 527
            |.|:|||                |::..|....:...|:.:..:.....|  .:||.|:|..|
Zfish   809 SMSTSSS----------------PSIHHRETLSKSLSLSPSHSDGSTETL--SVAAMDEVDEV-- 853

  Fly   528 ILKRSNVPAPNNDHDPSYGFASRYSDSKSYFRAHNVDNTIPTFVKREDLFNGDNMFPVHLKSHEE 592
                                               |.:|:.|...|..|.:.|:......|:.:.
Zfish   854 -----------------------------------VGSTVQTASMRNSLLSDDSFSTSDSKTLQA 883

  Fly   593 ----ICT--PPQKKRVKFDDTLDAVSP 613
                |.|  .|:.:.:..|.....:||
Zfish   884 LGDLIVTDLDPESRSLSIDSAYGTLSP 910

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	45/206 (22%)
		PH-like	269..>311	CDD:302622	10/41 (24%)
		PH	277..374	CDD:278594	24/109 (22%)
plekhg5a	XP_009304320.1	UNC-79	<52..>126	CDD:291442
		RhoGEF	440..626	CDD:279015	41/185 (22%)
		PH_PLEKHG5_G6	683..781	CDD:270064	25/109 (23%)
		PH	685..779	CDD:278594	23/105 (22%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810