Protein Alignment RhoGEF4 and arhgef18b

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_690750.6
• Gene: arhgef18b / 562264
• ZFINID: ZDB-GENE-070705-462
• Length: 1059
• Species: Danio rerio
• Alignment Length: 624
• Identity: 115/624 - (18%)
• Similarity: 232/624 - (37%)
• Gaps: 136/624 - (21%)

  Fly    71 RRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLREL-EA 134
            |:..|.|:|.:|..::..|::::..:.|.|:|...:|......||.:::.:..|:..||..| |.
Zfish   152 RQDVIYELIQTEIHHVRTLKIMLRVYARELREALQLDERRLDCLFPRLDSLLELHNHFLSRLRER 216

  Fly   135 NMENVAHAFLKMAPFFKLYSVYAFDYRGAL-----FIIQDLISKN-PVFRKFLEQTESRPEVQ-- 191
            .:|::.....:.....||..:....:.|.|     ....|..|:: .....:.||.:|..:.|
Zfish   217 RVESLQPGSERNYAINKLSDILISQFSGELGDRMKESYGDFCSRHTEAVNHYKEQLQSNKKFQNL 281

  Fly   192 -RKLNSLMIV-----------PIQRVPRYKLLLEQVLLYTSPADADYK-------LLKESVKEIE 237
             |||::|.||           ..||:.:|.:|||::|..|.....:::       |:|:.:.:::
Zfish   282 MRKLSNLSIVRRLGVPECILLVTQRITKYPVLLERILHNTEAGTEEHEGLAQALGLIKDVIVQVD 346

  Fly   238 ATASHINTCVEEQEIT-----QYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQKITHKGTEIKRYC 297
            |..|........:||.     :.|..:::..|.|..::.:..|:::.||.:......|.......
Zfish   347 AQVSLYEKEARLREIASKMEPKSLGKIKDGRVFRKEDLSQGRRKLLFEGTVNWKAASGRLKDILA 411

  Fly   298 VLMSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGN--FKLTCQSDGIIFGSGDV 360
            :|::|:.:..:...:|...:.|:|...   :..|:|..|.|:....  ..|.|.|      |.:.
Zfish   412 LLLTDVLVLLQEKDQRYVFSTVDNKPS---VISLQKLIVREVAHEERAMFLICAS------SNEP 467

  Fly   361 QL----------SRTWVGFIRDAIDL--HVQCRKTLRKDSSKRTPIRKKDMKKFGADYVLSPNKR 413
            ::          ..||:..||.|::.  |.:.|....::.::...:|
Zfish   468 EMYEIHTASKEDRNTWITHIRQAVESCPHTEERLFSEEEEARVARVR------------------ 514

  Fly   414 KCEYDTVFRNKNRSTDSEEETEDSACFSRKRKVASGILRAPNGNPMGKASSSSSSSVSMKRVAPP 478
              ||......|:... ::...|....|:...:..:|:           ..|.|.|.:.::
Zfish   515 --EYQERLALKDAQI-TQSLAEKLQLFAELSECVAGL-----------EDSGSRSRLLLR----- 560

  Fly   479 PPAPPPPPAVQMRHQPGRPGELATASKENRISKLYKKIAAGDKVANVRGILK---RSNVPAPNND 540
                               |:.:...:...:.|     ||..:|.|::.:|.   |........:
Zfish   561 -------------------GDTSDLQQGETLLK-----AAITEVENLQNLLTSRVRGTSAQTEGN 601

  Fly   541 HDPSYGFASRYSDSKSYFRAHNVDNTIPT--FVKREDLFNGDNMFPVHLKSHEEICTPPQKKRV- 602
            ..||.....|.:|:...:.:::  :.:|.  .:|::....|...    ....:...:.||.|.|
Zfish   602 QSPSLSLLPRRADTFGGYDSNS--SALPKNGSIKKKGPGAGGRT----RDRSQRASSDPQLKEVF 660

  Fly   603 --KFDDTLDAVSPVLVEKPFAFSSESSSALKPTSLRERI 639
              :..|.||..|     .|.:.:..||..|..:.|.||:
Zfish   661 TCQDHDELDEAS-----SPASTNMWSSCKLPESELYERV 694

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	45/197 (23%)
		PH-like	269..>311	CDD:302622	6/41 (15%)
		PH	277..374	CDD:278594	20/108 (19%)
arhgef18b	XP_690750.6	RhoGEF	152..346	CDD:238091	44/193 (23%)
		PH-like	388..497	CDD:302622	22/117 (19%)
		PH	<438..492	CDD:278594	13/62 (21%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910