Protein Alignment RhoGEF4 and arhgef33

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_009291341.1
• Gene: arhgef33 / 559216
• ZFINID: ZDB-GENE-131121-574
• Length: 714
• Species: Danio rerio
• Alignment Length: 640
• Identity: 131/640 - (20%)
• Similarity: 219/640 - (34%)
• Gaps: 187/640 - (29%)

  Fly    14 IHSPQTPLSFSRELRQVLQER----------NLLTAKSRRKVCSMLDSNNSSPIGSPNP----DA 64
            :.|.|..|....:|.|..|.|          ...:..|:.:: |::....||...:|||    |.
Zfish    93 VRSNQKDLQRDLDLMQSTQHRKGSRGEGSVDGHASLNSQTEI-SLIQHYISSLSANPNPFPEHDG 156

  Fly    65 GKR-----------------------LGFRRQAIQ-EIISSEKSYLEQLELLMNFFVR------- 98
            .|:                       ...:|||.. |::.||:.|:..|.||:...:.
Zfish   157 QKKSPVWRERKNSRELNNSTIICSYVFSAQRQAAALELLESERVYVSYLSLLLKANISFNGSENV 221

  Fly    99 PLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLRELEANMENV-AHAFLKM----APFFKLYSVYAF 158
            .||::.....|...|:...:|::|.|..   |.|:::.:.: ...||::    :.|...|..|..
Zfish   222 NLKDKRPFPPSLRFLIQQHLELLHLLQE---RVLKSHWQGIMGDVFLRLTSKESDFLDHYVSYLR 283

  Fly   159 DYRGALFIIQDLISKNPVFRKFLEQTESRPEVQRKLNSLMIVPIQRVPRYKLLLEQVLLYTSPAD 223
            |....|.::....|...  ...||...:..|.:..|.||::.|:||:|.|..||:.:|..|....
Zfish   284 DLPECLTVVSLFSSSKS--GSLLESDMTGDETRPTLYSLLLQPVQRIPEYHTLLQSLLQQTESEH 346

  Fly   224 ADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQKITH 288
            .||.||..||:::.:.            :|||     |.|:.....::..|.      .|::.||
Zfish   347 PDYYLLLVSVQQLRSF------------MTQY-----NHLLQHNQELLTHSH------ALREHTH 388

  Fly   289 KGTEIKRYCVLMSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGNFKLTCQSDGI 353
            .                .|...:||..:|..|          |.:..|.::    :|:..:.:
Zfish   389 N----------------LCTHQQERPSHTRKE----------LSRSTVRQL----YKVDYEKN-- 421

  Fly   354 IFGSGDVQLSRTWVGFIRDAIDLHVQCRKTLRKDSSKRTPIRKKDMKKFGADYVLSPNKRKCEY- 417
             :.|...|                        .|:|:|.       |:: .||..:|.....|.
Zfish   422 -YSSSSTQ------------------------NDASRRN-------KRY-PDYEAAPYHYDPEVP 453

  Fly   418 -------DTVFRNKNRSTD--SEEETEDSACFSRKRKVASGILRAPNG--NPMGKASS------- 464
                   |:..|:|..|..  |..|||.          |..:|....|  .|.|...|
Zfish   454 SPVSFLSDSDSRHKAISVPLRSIPETEG----------AGSVLADALGPLYPYGAGDSRCSSPSH 508

  Fly   465 SSSSSVSMKRVAPPPPAPPPPPAVQMRHQPG--------RPGELATASKENRISKLYKKIAAGDK 521
            ||.||:.:..|...|...||    :..|..|        ||.....:.....|.:.....|...|
Zfish   509 SSDSSIDIAFVRCSPSHSPP----RQSHNRGRSAGGVVYRPNRTCVSPDSADIVRPRPLQAVQRK 569

  Fly   522 VANVRGILKRSNVPAPNND-HDPSYGFASRYSDSKSYFRAHNVDNTIPTFVKRED 575
            ..::.| |:..::.:.|:. .:|::....|.|..|:..||.:..:...|....|:
Zfish   570 SKSLNG-LQMDSIDSLNSQTKNPAHPRLERQSSGKARLRASSPKHRYETHTDTEE 623

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	46/182 (25%)
		PH-like	269..>311	CDD:302622	5/41 (12%)
		PH	277..374	CDD:278594	13/96 (14%)
arhgef33	XP_009291341.1	RhoGEF	190..367	CDD:321997	48/198 (24%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170580161
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.930